Time filter

Source Type

Fu S.,Binzhou Animal Husbandry and Veterinary Research Academy | Fu S.,Shandong Lvdu Ante Veterinary Drug Industry Co. | Qu G.,Binzhou Animal Husbandry and Veterinary Research Academy | Qu G.,Shandong Lvdu Biological Technology Co. | And 10 more authors.
Applied Biochemistry and Biotechnology | Year: 2011

During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification. © 2010 Springer Science+Business Media, LLC.

Xu Q.,Shandong Academy of Sciences | Xu Q.,Shandong Lvdu Ante Veterinary Drug Industry Co. | Li J.,Northeast Agricultural University | Shen Z.,Shandong Academy of Sciences | And 6 more authors.
Journal of Chromatographic Science | Year: 2014

An HPLC-MS method has been developed and validated for the quantitative determination of milbemycin oxime (MBO) in dog plasma. The developed method has been then applied in in vivo clinical studies to obtain pharmacokinetics of MBO in blood after its oral administration. Samples were extracted using solid-phase extraction (SPE). Sample proteins were precipitated with acetonitrile (ACN) and sodium chloride (NaCl) and then diluted with methanol and water. Calibration standards were prepared by using plasma matrix and following the same SPE procedure. Chromatographic separation was performed on a Waters C18 packed column (3.5 μm particles diameter; 3 × 100 mm) with a C18 guard column (3.5 μm particles diameter; 3 × 20 mm). The mobile phase was an 85:15 (v/v) mixed solution of ACN and 5 mM ammonium acetate. The calibration curve was linear over a concentration range of 2.0-500 ng/mL with a limit of quantitation of 2.0 ng/mL. The oral administration of a pellet of 2.5 mg MBO produced blood concentrations ranging from 6.10 ± 0.92 to 78.81 ±4.38 ng/mL within 6 h, with a terminal half-time of 11.66 ±0.93 h. This study determined the suitability of the herein proposed method to investigate the pharmacokinetics of MBO after oral administration. © The Author [2013]. Published by Oxford University Press. All rights reserved.

Xu Q.,Shandong Academy of Sciences | Xu Q.,Shandong Lvdu Ante Veterinary Drug Industry Co. | Wang Y.,Binzhou Medical College Hospital | Guo S.,Shandong Academy of Sciences | And 7 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Flos populi is an important traditional Chinese medicine prepared from the male inflorescence of Populus tomentosa Carr. or Populus canadensis Moench (Salicaceae family). Flos populi is mainly used for the treatment of various inflammatory diseases and anti diarrhea in East Asian countries. The objective of this study was to investigate the anti-inflammatory and analgesic properties of the aqueous extract of Flos populi (FPAE). Materials and methods Cotton pellets-induced granuloma, carrageenan-induced paw oedema, arachidonic acid-induced right ear oedema and xylene-induced ear oedema were used to assess anti-inflammatory activity of FPAE and analgesic activity was evaluated by hot plate test, acetic acid-induced abdominal writhing test and formalin test. Results FPAE produced significant dose-response anti-inflammatory activity against cotton pellets-induced granuloma. FPAE at dosages of 50, 100 and 200 mg/kg b w. significantly reduced carrageenan-induced paw edema by 48.84% (P<0.05), 54.95% (P<0.05), and 62.05% (P<0.05) at 5 h after carrageenan injection, respectively. FPAE significantly (P<0.05) reduced the ear oedema induced by arachidonic acid and peaked at the dose of 200 mg/kg b w. (40.78%). A significant (P<0.05) dose dependent inhibition of xylene-induced oedema was produced by FPAE and peaked at the highest dose of 200 mg/kg b w. (23.95%). FPAE (50, 100 and 200 mg/kg b w.) produced significant dose-response analgesic activity in the hot-plate test. However, the low percentage inhibition (<50%) suggests that it is not a centrally acting analgesic. Extract at dosages of 50, 100 and 200 mg/kg b w., p.o. significantly reduced acetic acid-induced writhing by 39.6% (P<0.05), 45.4% (P<0.05), and 51.8% (P<0.05), respectively. The extract also caused marked dose-dependent inhibition of formalin-induced pain in the second phase (P<0.05). Conclusion The findings in this study suggest that the aqueous extract of Flos populi possesses anti-inflammatory and analgesic activities. These results may support the fact that this plant is used traditionally to cure inflammatory diseases. © 2014 Elsevier Ireland Ltd.

Loading Shandong Lvdu Ante Veterinary Drug Industry Co. collaborators
Loading Shandong Lvdu Ante Veterinary Drug Industry Co. collaborators