Liu X.-M.,Shandong Academy of Agricultural Sciences |
Liu X.-M.,Shandong Key Laboratory Ofanimal Disease Control and Breeding |
Song E.-L.,Shandong Academy of Agricultural Sciences |
Song E.-L.,Shandong Key Laboratory Ofanimal Disease Control and Breeding |
And 9 more authors.
Progress in Biochemistry and Biophysics | Year: 2010
Sirtuin type 1 (SIRTl), a novel regulatory factor of adipocyte and myocyte, interacts with target the forkhead box O family 1 (FoxOl) to modulate cell proliferation and differentiation, aging, apotosis and metabolism. The effect of nicotinamide (NAM, inhibitor of SIRTl) on the apoptosis of bovine preadipocytes was investigated with Acridine Orange (AO) staining, the flow cytometry detection and quantitative real-time PCR (qPCR). The results showed that NAM inhibited the differentiation of bovine subcutaneous preadipocytes (BSP) and bovine intramuscular preadipocytes (BIP), and their apoptosis rates were higher than control. SIRTl influenced the apoptosis of bovine preadipocytes by regulating FoxOl and adipocyte marker genes. The different mechanisms of SIRTl and its related genes exist in BSP and BIP.