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Yu T.,Shandong Jining No1 Peoples Hospital | Zuber J.,University of Massachusetts Amherst | Li J.,University of Massachusetts Amherst
Journal of Molecular Medicine | Year: 2015

Autophagy is a major intracellular degradative process by which cytoplasmic materials are sequestered in double-membraned vesicles and degraded upon fusion with lysosomes. Under normal circumstances, basal autophagy is necessary to maintain cellular homeostasis by scavenging dysfunctional or damaged organelles or proteins. In addition to its vital homeostatic role, this degradation pathway has been implicated in many different cellular processes such as cell apoptosis, inflammation, pathogen clearance, and antigen presentation and thereby has been linked to a variety of human disorders, including metabolic conditions, neurodegenerative diseases, cancers, and infectious diseases. The skin, the largest organ of the body, serves as the first line of defense against many different environmental insults; however, only a few studies have examined the effect of autophagy on the pathogenesis of skin diseases. This review provides an overview of the mechanisms of autophagy and highlights recent findings relevant to the role of autophagy in skin diseases and strategies for therapeutic modulation. © 2014 Springer-Verlag Berlin Heidelberg.


Zhang Y.,Shandong Jining No1 Peoples Hospital | Luo L.,Shandong Jining No1 Peoples Hospital | Luo Q.,Shandong Jining No1 Peoples Hospital
Journal of biological regulators and homeostatic agents | Year: 2015

This study was designed to explore tissue hardness and distinguish benign and malignant endometrial cancer with the use of transvaginal ultrasonography combined with elastography. Color Doppler ultrasonic diasonograph was used to carry out transvaginal ultrasonography and elastography. Once the nidus was observed, features of the 2D image were analyzed. Then features of elasticity of the uterine cavity in different states were analyzed by elastography, and strain rate ratio was measured. Finally, elasticity scoring (0~5 points) was made. Receiver operating characteristic (ROC) curve was drawn based on elasticity score and strain rate ratio. The area under the elasticity score curve and strain rate ratio curve was 0.761 and 0.852, respectively, and there was no statistically significant difference between them (c2= 4.663, P>0.05). Then 2.98 was confirmed as the diagnostic cut-off value of benign and malignant lesions, based on strain rate ratio. Ultrasonic elastography as an effective assistance for transvaginal ultrasonography provides more valuable information for confirmation of lesions and offers more accurate evidence for diagnosis of disease in the uterine cavity.


Jia H.-X.,Shandong Jining No1 Peoples Hospital | Xun X.-H.,Shandong Jining No1 Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016

Nowadays, the most important social and economic claims China faces, is the hasty aging of its citizens. China has a large aged population now, although it is still deemed as a middle-income country. Coupled with the fast erosion of family support and market-driven restructuring social services, the condition of accessible and af­fordable social care services to aged society has already turn into an vital concern for the Chinese government to address. The study aims to understand what aged Chinese citizens with chronic illness and their ancestor’s caregiv­ers perceive to be good care, and to evaluate point of views of those living in countryside and municipal areas. The total mean score of instrumental activities of daily living at the early days of care and discharge was found to be 2.12 and 2.80, correspondingly, disclosing a significant enhance of 2.12 (t = 12.72, P < .001). The inner reliability of the instrument was counted using Cronbach’s Alpha with a ranging between 0.77 and 0.88, except for cognitive functioning (α = 0.49) and emotional functioning (α = 0.64). The results of multilevel regression models examining the association between total POSM-NF scores and covariates. A greater ADL Hierarchy Index was associated with a lower total POSM scores (parameter estimate: -.728, P = .04). Therefore, residents with impaired physical inde­pendence were more likely to report lower QoL. Among residents who reported depression, had lower total POSM score as well (parameter estimate: -3.015, P-.01). The association between nursing homecare’s overall star rating and QoL was not significant (parameter estimate = 0.683; P = .12). © 2016, E-Century Publishing Corporation. All rights reserved.


Zhang Y.,QiLu Hospital | Lv Y.,QiLu Hospital | Zhang Y.,Shandong Jining No1 Peoples Hospital | Gao H.,QiLu Hospital
PLoS ONE | Year: 2014

p53 is possibly the most important mammalian tumor suppressor and it is mutated or lost in more than half of all human cancers. The stability of p53 is primarily determined by the RING domain E3 ubiquitin ligase Hdm2, which targets p53 for proteasomal degradation, restraining the potent activity of p53 and enabling cell survival and proliferation. UBE4B has been shown to physically interact with p53 and Hdm2 and to negatively regulate p53 stability and function. However, no one has determined whether UBE4B promotes p53 degradation in breast cancer. In this study, UBE4B promoted the degradation and ubiquitination of p53 to inhibit the apoptosis of cancer cells and promote tumorigenesis. Our results indicate that UBE4B regulates p53 in breast cancer and could be a viable target for developing new therapeutic strategies for breast cancer treatment. © 2014 Zhang et al.


Zhang Y.,Shandong Jining No1 Peoples Hospital | Ma A.D.,Shandong Jining No1 Peoples Hospital | Jia H.X.,Shandong Jining No1 Peoples Hospital
Journal of biological regulators and homeostatic agents | Year: 2016

This study explores the correlation between molybdenum target (mo-target) mammography signs and pathological prognostic factors of breast cancer. We selected 320 breast cancer patients who were treated between January 2014 and January 2016; using single-factor and multiple-factor logistic regression method, we made correlation analysis on their clinical features, pathological features and mo-target mammography signs. Among mo-target mammography signs, lumps accompanied with calcification and blurry edge were associated with high histologic grades; lumps accompanied with calcification and clear edge were associated with Ki-67 positive; compared with the patients who had lumps with non-stellate edges, positive rates of estrogen receptor (ER) and progesterone receptor (PR) were significantly higher for the patients who had lumps with stellate edges (p < 0.01), while positive rate of human epidermal growth factor receptor-2 (HER-2) and tumor proliferative activity were significantly lower (p < 0.05, p < 0.01). According to the study, we can conclude that mo-target mammography signs mainly include lumps and calcification. Mo-target mammography can improve the accuracy of diagnosis and reduce misdiagnosis or missed diagnosis. Part of mo-target mammography signs are associated with clinical pathology prognostic factors; by grasping the relation, breast cancer patient conditions are expected to be relieved.


Lu Q.,Shandong Jining No1 Peoples Hospital | Liu H.,Shandong Jining No1 Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2014

The main objective of this study is to investigate the relationship between ascending aorta elasticity and the severity of coronary artery stenosis in essential hypertensive patients with coronary heart disease (CHD) using M-mode and tissue Doppler echocardiography. A total of 184 hypertensive patients with CHD were enrolled. Patients were divided into three groups based on the severity of coronary stenosis measured by coronary arteriography (CAG): slight stenosis (group 1), moderate stenosis (group 2) and serious stenosis (group 3). M-mode and tissue Doppler echocardiography were performed, and elasticity indexes of ascending aorta including stiffness index, distensibility index, and S wave speed of anterior wall were calculated and correlated with the severity of coronary stenosis. Ascending aorta stiffness index was increased, whereas distensibility index and S wave speed of anterior wall were decreased in moderate and severe stenosis groups compared with slight stenosis group (P < 0.01). Elasticity indexes change in a stepwise pattern with the narrowness of coronary artery, and there was a significant correlation between aortic elasticity and severity of coronary artery by Pearson correlation analysis (P < 0.01). Elasticity indexes of ascending aorta correlate well with severity of coronary stenosis. Elasticity indexes of ascending aorta can serve as predictors for coronary arterial lesion in hypertensive patients. © 2014, Springer Science+Business Media New York.


Wang M.,Shandong Jining No1 Peoples Hospital | Yu T.,Shandong Jining No1 Peoples Hospital | Zhu C.,Guangdong Medical College | Sun H.,Shandong Jining No1 Peoples Hospital | And 3 more authors.
Nutrition and Cancer | Year: 2014

Resveratrol (3,5,4′-trihydroxy-trans-stilbene, RSV), a natural polyphenolic compound, is known as a promising anti-cancer agent. In this study, we showed that RSV could inhibit the growth of B16 cells via induction of apoptosis. Moreover, our results showed for the first time that RSV induced autophagy in B16 cells, which might occur through ceramide accumulation and Akt/mTOR pathway inhibition. Inhibition of autophagy by an autophagic inhibitor 3-methyladenine (3-MA) or si-Beclin 1 enhanced RSV-induced cytotoxicity and apoptosis. Thus, autophagy inhibition represents a promising approach to improve the efficacy of RSV in the treatment of patients with melanoma. © 2014 Taylor and Francis Group, LLC.


PubMed | Weifang Medical University, Shandong Jining No1 Peoples Hospital, Qingdao University and Jinan Maternal and Child Care Hospital
Type: Journal Article | Journal: Pharmaceutical development and technology | Year: 2016

Etomidate is a unique drug used for induction of general anesthesia and sedation, and is usually used through intravenous injection clinically. Before targeting to the receptor, etomidate binds proteins in blood when it comes into veins. Thus to study the interaction of etomidate and serum albumin would be of great toxicological and pharmacological importance. In this study, the interaction between etomidate and human serum albumin (HSA) was studied using fluorescence spectroscopy, UV-vis absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR), circular dichroism (CD) spectroscopy, site maker displacement and molecular modeling methods. Investigations of the binding constant (K=3.5510(5)M(-1), 295K), the number of binding sites (n=1.16), thermodynamic parameters (G=3.1310(4)Jmol(-1), S=364Jmol(-1)K(-1) and H=-6.8510(5)Jmol(-1)) for the reaction and changes to the binding sites and conformation in HSA in response to etomidate were presented. Results show that etomidate can bind HSA tightly through electrostatic forces, and the protein skeleton conformation and secondary structure changes thereby. This is the first spectroscopic report for etomidate-HSA interactions which illustrates the complex nature of this subject.


Wang L.,Shandong Jining No1 Peoples Hospital | Lin Y.,Shandong Jining No1 Peoples Hospital | Qi C.-C.,Shandong Jining No1 Peoples Hospital | Sheng B.-W.,Shandong Jining No1 Peoples Hospital | Fu T.,Shandong Jining No1 Peoples Hospital
Asian Pacific Journal of Cancer Prevention | Year: 2014

The X-ray repair cross-complementing group 1 protein (XRCC1) plays important roles in the DNA base excision repair pathway which may influence the development of lung cancer. This study aimed to evaluate the potential association of the XRCC1 c.1178G>A genetic polymorphism with lung cancer risk. The created restriction site-polymerase chain reaction (CRS-PCR) and DNA sequencing methods were utilized to evaluate the XRCC1 c.1178G>A genetic polymorphism among 376 lung cancer patients and 379 controls. Associations between the genetic polymorphism and lung cancer risk were determined with an unconditional logistic regression model. Our data suggested that the distribution of allele and genotype in lung cancer patients was significantly different from that of controls. The XRCC1 c.1178G>A genetic polymorphism was associated with an increased risk of lung cancer (AA vs GG: OR=2.91, 95%CI 1.70-4.98, p<0.001; A vs G: OR=1.52, 95%CI 1.22-1.90, p<0.001). The allele A and genotype AA may contribute to risk of lung cancer. These preliminary results suggested that the XRCC1 c.1178G>A genetic polymorphism is statistically associated with lung cancer risk in the Chinese population.


PubMed | Shandong Jining No1 Peoples Hospital and Jining Medical University
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016

MiRNAs, as oncogenes or as anti-oncogenes, play critically regulated roles in human cancers at posttranscriptional level. A number of dysregulated miRNAs has been observed in HCC. However, the expression and function of miR-670-5p have not been evaluated in HCC to date. In this study, we examined and confirmed the over-expression of miR-670-5p in HCC and in hepatoma-derived cells Hep3B. At least 60% of HCC tissues showed a greater than three-fold enhance in the expression of miR-670-5p compared with paired adjacent non-cancerous tissues. Knockdown studies for miR-670-5p showed that the expression of miR-670-5p promoted cellular proliferation. In tissues and cells with high expression of miR-670-5p, decreased expression of PROX1, a miR-670-5p predicated target, was detected. It confirmed that PROX1 expression was obviously affected by the expression of miR-670-5p. Furthermore, overexpression of PROX1 greatly inhibitted cellular proliferation. Therefore, it was inferred that miR-670-5p may play important roles in enhancing proliferation activity that is associated with HCC by modulating PROX1 expression at posttranscriptional level.

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