Wu Z.-Y.,Chinese PLA General Hospital |
Cao H.-L.,Shandong Jiaotong Hospital |
Deng M.-H.,Chinese PLA General Hospital |
Wu J.-Y.,Chinese PLA General Hospital
International Journal of Clinical and Experimental Medicine | Year: 2017
Aprepitant is one of the effective antiemetic drugs that usually used for prevention of Capecitabine and oxaliplatin (XELOX) chemotherapy-induced nausea and vomiting (CINV). We aimed to evaluate the effect of aprepitant on the control of CINV when conventional antiemetics failed. Patients with gastric and colon cancer scheduled to receive XELOX regimens were enrolled in this study, initially receiving 5-Hydroxytryptamine-3 (5-HT3) receptor antagonists and dexamethasone as anti-emetics. After patients experienced vomiting of grade ≥2 and required rescue anti-emetic drugs in the first cycle, oral aprepitant was added in second cycle. Acute (day 1) and delayed (days 2-5) CINV and occurrence of adverse reactions were investigated after the start of chemotherapy. Thirty patients (19.7%) were administered aprepitant for rescue project against CINV during the second cycle of chemotherapy. Delayed CINV were 100% during the first cycle but became lower in the second cycle, which revealed significant effectiveness of the addition of aprepitant on the control of delayed CINV when 5-HT3 receptor antagonists and dexamethasone failed. The incidences of acute and delayed nausea and vomiting in the first cycle of chemotherapy were significantly higher than the second cycle added aprepitant as rescue antiemetic (P<0.05). The incidences of nausea and vomiting were significantly lower after taken the rescue medication aprepitant. Addition of aprepitant to 5-HT3 antagonists and dexamethasone resulted in significantly better prevention of nausea and vomiting than the first cycle for gastric and colon cancer patients receiving XELOX chemotherapy. © 2017, E-Century Publishing Corporation. All rights reserved.
Wang T.,Shandong Jiaotong Hospital |
Wang B.,Hangzhou First Peoples Hospital
Molecular Medicine Reports | Year: 2016
The present study integrated microRNA (miRNA) and mRNA expression data obtained from atrial fibrillation (AF) tissues and healthy tissues, in order to identify miRNAs and target genes that may be important in the development of AF. The GSE28954 miRNA expression profile and GSE2240 mRNA gene expression profile were downloaded from the Gene Expression Omnibus. Differentially expressed miRNAs and genes (DEGs) in AF tissues, compared with in control samples, were identified and hierarchically clustered. Subsequently, differentially expressed miRNAs and DEGs were searched for in the miRecords database and TarBase, and were used to construct a regulatory network using Cytoscape. Finally, functional analysis of the miRNA-targeted genes was conducted. After data processing, 71 differentially expressed miRNAs and 390 DEGs were identified between AF and normal tissues. A total of 3,506 miRNA-mRNA pairs were selected, of which 372 were simultaneously predicted by both miRecords and TarBase, and were therefore used to construct the miRNA-mRNA regulatory network. Furthermore, 10 miRNAs and 12 targeted mRNAs were detected, which formed 14 interactive pairs. The miRNA-targeted genes were significantly enriched into 14 Gene Ontology (GO) categories, of which the most significant was gene expression regulation (GO 10468), which was associated with 7 miRNAs and 8 target genes. These results suggest that the screened miRNAs and target genes may be target molecules in AF development, and may be beneficial for the early diagnosis and future treatment of AF.
Wang T.,Shandong Jiaotong Hospital |
Dong A.-H.,Peoples Hospital of Linzi District |
Cao H.-Y.,Peoples Hospital of Linzi District
Genetic Testing and Molecular Biomarkers | Year: 2016
Context: Slow coronary flow (SCF) is a special coronary microvascular disorder associated with recurrent chest pain. The pathogenesis of SCF remain unclear. Objectives: We sought to assess whether serum salusin-β levels are correlated with SCF. Methods: We enrolled 76 patients with angiographically confirmed SCF and 108 age- and gender-matched controls. We measured serum salusin-β levels by enzyme-linked immunosorbent assay and coronary flow rate was assessed using thrombolysis in myocardial infarction frame count (TFC). Results: Serum salusin-β levels were elevated in SCF patients compared with controls (4.33 [range 3.52-5.87] nmol/L vs. 3.76 [range 2.98-4.67] nmol/L). Multivariate logistic regression analysis revealed that salusin-β in serum was the independent predictor of SCF (odds ratio = 1.814). Serum salusin-β levels were independently correlated with mean-TFC (r = 0.355, p = 0.002). Conclusions: Serum salusin-β levels were independently associated with SCF. Therefore, our findings implicate a potential role of salusin-β in the pathophysiology of SCF and provide insights on both risk stratification and modification in this patient population. © Copyright 2016, Mary Ann Liebert, Inc.
Shan Y.L.,Shandong Jiaotong Hospital
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2011
To compare the efficacy differences between electroacupuncture and medication in lumbar disc herniation. Eighty-five cases of lumbar disc herniation were randomly divided into an electroacupuncture group (45 cases) and a medication group (40 cases). The electroacupuncture group was treated with electroacupuncture at Jiaji (EX-B 2), Shenshu (BL 23), Qihaishu (BL 24), Guanyuanshu (BL 26), Dachangshu (BL 25) and Yangtlingquan (GB 34) etc., once a day; and the medication group was treated with oral administration of Fugui Gutong capsule (3 times a day, 4 capsule each time) and 0.3 g ibuprofen (once a day). The scores of clincial symptoms and therapeutic effect were observed before and after treatment. Results In the electroacupuncture group, the effective rate was 84.4% (38/45), which was superior to that of 65.0% (26/40) in the medication group (P < 0.05). After treatment, the scores of symptoms significantly decreased in the two groups (both P < 0.01), and the reduction of scores in electroacupuncture group was superior to that in medication group (P < 0.05, P < 0.01). Electroacupuncture at Jiaji (EX-B 2) and points of Bladder Meridian mainly has a better therapeutic effect on lumbar disc herniation, which is superior to oral administration of fugui gutong capsule and ibuprofen.
Zhang R.,Shandong Jiaotong Hospital |
Xu Q.,Shandong Jiaotong Hospital
Tropical Journal of Pharmaceutical Research | Year: 2016
Purpose: To evaluate the curative effect of corticosteroids in the treatment of acute pain, local edema, and skin lesions caused by herpes zoster, and to develop some pertinent therapeutic guidelines. Methods: A total of 48 cases of patients diagnosed with herpes zoster from 2010 to 2011 in the dermatology clinic of Shan Dong Traffic Hospital were selected and all received the same therapy of antiviral, pain-relieving and nerve nutrition. They were divided into a corticosteroid application group, with 24 patients treated with corticosteroids, and a control group of 24 patients without corticosteroids. Local swelling subsided in the corticosteroid group. The differences observed in pain relief and days needed for blisters to dry and scab between the two patient groups were analyzed to determine significance and, thus, assess the curative effect of corticosteroids in treatment of herpes zoster. Results: Patients in the glucocorticosteroid application group relieved pain faster than patients in the control group (2.38 ± 1.41 days vs 5.50 ± 3.19 days), and the difference was significant (p < 0.05). Skin lesions of patients in the glucocorticosteroid application group healed quicker than that of patients in control group (2.83 ± 0.87 days vs 3.54 ± 1.02 days), and the difference was remarkable (p < 0.05). Local swelling of patients in the glucocorticosteroid application group recovered rapidly after treatment.. Conclusion: Treatment of herpes zoster with appropriate corticosteroid isodose application can effectively relieve acute pain and local swelling, and speed up scabbing and healing of skin lesions. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Ma Y.,Shandong JiaoTong Hospital |
Sui Y.,Second Peoples Hospital of Jinan City |
Wang L.,Shandong JiaoTong Hospital |
Li H.,Shandong JiaoTong Hospital
Tumor Biology | Year: 2014
Glutathione S-transferases are important enzymes in the detoxification of a wide range of reactive oxygen species. Recently, there have been a number of studies on the association between Glutathione S-transferase M1 (GSTM1) null genotype and childhood acute leukemia in Chinese, but the results of previous reports are inconsistent. Thus, we performed a meta-analysis to clarify the effect of GSTM1 null genotype on childhood acute leukemia risk. PubMed, Embase, and Wanfang databases were searched to identify case-control studies investigating the association between GSTM1 null genotype and risk of childhood acute leukemia. Between-study heterogeneity was assessed using the I2 statistic method. Odds ratios (OR) with corresponding 95% confidence intervals (95%CI) were pooled to assess the association. Seven case-control studies were finally included in the meta-analysis. There was no between-study heterogeneity among those seven studies (I2=0 %). Overall, the GSTM1 null genotype was significantly associated with increased risk of childhood acute leukemia in Chinese (fixed effect OR=2.49; 95 % CI, 1.84-3.37; P<0.001). The cumulative meta-analyses showed a trend of more obvious association between GSTM1 null genotype and risk of childhood acute leukemia in Chinese as data accumulated by year. Sensitivity analysis by omitting single study in turns also did not materially alter the pooled ORs. Therefore, the GSTM1 null genotype is significantly associated with increased risk of childhood acute leukemia in Chinese.
Guo L.-M.,Shandong University |
Xi J.-S.,Shandong University |
Ma Y.,Shandong Jiaotong Hospital |
Shao L.,Shandong Jiaotong Hospital |
And 2 more authors.
Tumor Biology | Year: 2014
Childhood acute lymphoblastic leukemia (ALL) is the leading cause of cancer-related deaths among children. Two recent genome-wide association studies and several replicated studies have provided convincing evidence that inherited genetic variation in ARID5B contributes to childhood ALL predisposition. In the present study, we performed a meta-analysis to systematically summarize the association between ARID5B genetic polymorphism and the risk for ALL. We conducted a search of case-control studies on the association of ARID5B genetic polymorphisms with susceptibility to ALL in PubMed, EMBASE, Wanfang database in China, and Chinese National Knowledge Infrastructure databases. Data from eligible studies were extracted for metaanalysis. ALL risk associated with ARID5B genetic polymorphism was estimated by pooled odds ratios (ORs) and 95 % confidence intervals (95 % CIs). Nine articles including 13 case-control studies were included in the present metaanalysis. We found that rs10821936 polymorphism in ARID5B gene was associated with increased risk for ALL (P <0.0001; OR=1.27; 95 %CI, 1.17-1.37). This metaanalysis suggests that ARID5B genetic polymorphism was associated with the increased risk of ALL.
Liu B.,Jinan Fourth Hospital |
Qiu P.,Shandong University of Traditional Chinese Medicine |
Chen H.,Shandong Jiaotong Hospital |
Li Q.,Shandong University
Artery Research | Year: 2014
Objective: To compare the simultaneous invasive and non-invasive measurements of blood pressure (IBP and NIBP) based upon the Multiparameter Intelligent Monitoring in Intensive Care II (MIMIC II) database. Methods and results: A total of 986 records and 26,942 blood pressure (BP) measurements were extracted from MIMIC II database. The mean values of invasive systolic and invasive diastolic blood pressure (ISBP and IDBP) were 111.2 ± 33.9 mm Hg and 59.9 ± 22.8 mm Hg respectively, and the values of non-invasive ones were 114.0 ± 23.4mmHg and 51.0 ± 14.9 mm Hg. The average differences of systolic and diastolic blood pressure were -2.8mmHg and 8.9mmHg between IBP and NIBP. The correlation coefficients were 0.60 between ISBP and NISBP and 0.45 between IDBP and NIDBP. The robust regression equations between IBP (y) and NIBP (x) showed y = 1.02x - 2.95 for SBP (R2=0.60) and y = 0.77x + 18.43 for DBP (R2 = 0.82). At the higher part of BP, IBP is larger than NIBP, and at the lower part of BP, IBP is less than NIBP. Conclusion: Average invasive systolic blood pressure is lower than the non-invasive one and average invasive diastolic blood pressure is higher than the non-invasive one. The IBP shows good correlation with the NIBP. The invasive blood pressures can be estimated from non-invasive ones by the regression equations. © 2014 Association for Research into Arterial Structure and Physiology.
PubMed | Shandong University and Shandong Jiaotong Hospital
Type: Journal Article | Journal: World journal of surgical oncology | Year: 2017
Gliomas are one of the most common malignant brain tumors and bring a big threat to human life as traditional therapy is unsatisfactory. RBM5 was a RNA-binding motif protein and was reported as a tumor suppressor. But the role of RBM5 in gliomas was unknown.The mRNA level of RBM5 was determined in gliomas tissues and cell lines by real-time quantitative PCR (qRT-PCR) assay while the association of RBM5 expression with prognosis was analyzed by Kaplan-Meier method and compared by log-rank test. Lentivirus was used to overexpress RBM5 in gliomas cells. MTT and BrdU incorporation assay were used to determine cell proliferation and DNA synthesis when the ability of cell migration and invasion was analyzed by transwell assay with/without Matrigel. Cell apoptosis rate was determined with fluorescence-activated cell sorting (FACS) method. Then, expression of apoptosis molecules and critical members in Wnt/-catenin pathway were detected by western blot analysis.RBM5 was shown to be downregulated in gliomas tissues and gliomas cell lines. And decreased RBM5 expression was clinically correlated with tumor stage, patient age, and poor prognosis of gliomas patients. The proliferation and DNA synthesis was dramatically inhibited when RBM5 was overexpressed in SHG44 or U251 cells. Also, the ability of cell migration and invasion was disrupted. Then, the level of -catenin and Cyclin D1 significantly decreased when DKK1 and P-GSK-3 increased reversely in SHG44 cells, which suggested that RBM5 inhibited canonical Wnt/-catenin signaling. Meanwhile, we demonstrated that caspase3-mediated apoptotic pathway was activated by RBM5 as Bax, TNF-, and cleaved caspase3 were greatly upregulated while antiapoptotic molecule Bcl-2 was downregulated. Additionally, that apoptotic rate increased significantly from less than 1 to 32% in RBM5-overexpressed SHG44 cells further supported the pro-apoptosis role of RBM5 in gliomas cells.RBM5 plays a suppressor role in human gliomas by inhibiting Wnt/-catenin signaling and inducing cell apoptosis. This study improves our knowledge about the carcinogenesis and progression of human gliomas, which would greatly contribute to the therapy for gliomas patients.
PubMed | Shandong Jiaotong hospital and Shandong University
Type: Journal Article | Journal: Journal of biological regulators and homeostatic agents | Year: 2017
This study aims to discuss the remission effect of vitamin C on isoflurane-induced apoptosis of rats and its possible mechanism of action, to provide a theoretical basis for postoperative cognitive impairment. Reactive oxygen species (ROS) detection, adenosine triphosphate (ATP) test, MTT method and Morris water maze were applied for detection tests. For data statistics, double factor analysis of variance (ANOVA) and post hoc Bonferroni test were adopted. It was found that vitamin C could slow down the isoflurane-induced accumulation of ROS in H4-APP cells; moreover, it could relieve the activation of caspase-3 and increase cell survival rate to inhibit the occurrence of apoptosis, indicating that ROS was the source of cell toxicity. On the other hand, vitamin C could protect the cells with its antioxidant effect. It was proved that vitamin C could remit isoflurane-induced apoptosis and relieve the decline in learning and memory ability of rats.