Shandong Institute of Pomology Taian

China

Shandong Institute of Pomology Taian

China
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Yu X.M.,Shandong Institute of Pomology Taian | Zhou G.F.,Shandong Institute of Pomology Taian | Shan G.H.,Shandong Institute of Pomology Taian | Wang H.R.,Shandong Institute of Pomology Taian | And 3 more authors.
Acta Horticulturae | Year: 2013

The cultivar 'Jinsi 4' of Chinese jujube was used to establish a novel DNA extraction method for the detection of jujube witches' broom phytoplasmas. The result showed that the amplified fragment from the crude DNA extract of jujube JWB-infected leaves was the same as that from the total DNA extract using the conventional improved SDS-CTAB method, which was classified as a member of 16SrV-B subgroup. Then the cultivars 'Yuanling 1', 'Lubeidongzao' and 'Dabailing'of Chinese jujube were used to verify the applicability of the established novel DNA extraction method, the results showed that these 3 isolates were also classified as members of 16SrV-B subgroup. And the online virtual 16S rDNARFLP analysis showed that these 4 isolates (accession No.:HM989945, HM989946, HM989947 and HM989948) shared high identity with JWB-G1 (AB052876), their similarity coefficient were 0.98, 0.97, 0.98 and 0.98 respectively, which indicated that the novel DNA extraction method established in this study was practically used to detect jujube witches' broom phytoplasmas.


Sun Q.,Shandong Institute of Pomology Taian | Sun H.,Shandong Institute of Pomology Taian | Li G.,Shandong Institute of Pomology Taian | Shen G.N.,Shandong Institute of Pomology Taian | Zhou G.F.,Shandong Institute of Pomology Taian
Acta Horticulturae | Year: 2013

Healthy and phytoplasma-infected shoot cultures of two jujube cultivars 'Jinsi 4' (Ziziphus jujuba Mill.) and sour jujube (Ziziphus spinosus Hu.) were selected as materials. In vitro shoots proliferation and adventitious shoot induction from leaf explants of healthy and phytoplasma-infected jujube were examined. Results showed that the proliferation media for healthy and phytoplasma-infected shoots of the same cultivar were obviously different. Proliferation of phytoplasma-infected shoots was better than that of healthy shoots. Phytoplasma-infected 'Jinsi 4' jujube and sour jujube both showed proliferation well on hormone-free MS medium, but healthy 'Jinsi 4' jujube showed no proliferation on hormone-free MS medium (MS0) and a little proliferation on MS medium with 1-4 mg/L benzyladenine (BA) and 0.4 mg/L indole-3-butyric acid (IBA). Healthy sour jujube couldn't proliferate on MS0, but proliferated very well on MS medium with 2 mg/L BA and 0.4 mg/L IBA. Shoot regeneration from leaf explants of phytoplasma-infected shoots was easier than that of healthy shoots. The highest shoot regeneration rates of two cultivars' diseased leaves were obtained on WPM medium with lower TDZ concentration of 0.1 mg/L. The highest regeneration rate of healthy sour jujube leaves was obtained on WPM medium with higher TDZ concentration of 1 mg/L, and healthy 'Jinsi 4' jujube leaves failed to regenerate. The possible reason of the difference of proliferation and regeneration between healthy and phytoplasma-infected shoots is discussed.

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