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Cui Y.,Shandong University | Meng Y.,Shandong University | Zhang J.,Shandong Institute for Product Quality Inspection | Cheng B.,Shandong Institute for Product Quality Inspection | And 4 more authors.
Protein Expression and Purification | Year: 2017

In well-established heterologous hosts, such as Escherichia coli, recombinant proteins are usually intracellular and frequently found as inclusion bodies—especially proteins possessing high rare codon content. In this study, successful secretory expression of three hydrolases, in a constructed inducible or constitutive system, was achieved by fusion with a novel signal peptide (Kp-SP) from an actinomycete. The signal peptide efficiently enabled extracellular protein secretion and also contributed to the active expression of the intracellular recombinant proteins. The thermophilic α-amylase gene of Bacillus licheniformis was fused with Kp-SP. Both recombinants, carrying inducible and constitutive plasmids, showed remarkable increases in extracellular and intracellular amylolytic activity. Amylase activity was observed to be > 10-fold in recombinant cultures with the constitutive plasmid, pBSPPc, compared to that in recombinants lacking Kp-SP. Further, the signal peptide enabled efficient secretion of a thermophilic cellulase into the culture medium, as demonstrated by larger halo zones and increased enzymatic activities detected in both constructs from different plasmids. For heterologous proteins with a high proportion of rare codons, it is difficult to obtain high expression in E. coli owing to the codon bias. Here, the fusion of an archaeal homologue of the amylase encoding gene, FSA, with Kp-SP resulted in > 5-fold higher extracellular activity. The successful extracellular expression of the amylase indicated that the signal peptide also contributed significantly to its active expression and signified the potential value of this novel and versatile signal peptide in recombinant protein production. © 2016 Elsevier Inc.


Liu J.,Shandong University | Wang J.,Shandong University | Wang J.,Shandong Institute for Product Quality Inspection | Zhao C.,Shandong University | And 3 more authors.
Applied Microbiology and Biotechnology | Year: 2016

Triclosan (TCS) is widely used in consumer products as an antimicrobial agent. Constructed wetlands have the potential for TCS removal, but knowledge about the relative importance of sediment, plants, and microbes is limited. TCS removal performance was investigated in well-operated constructed wetlands planted with three different types of aquatic plants: emergent Cattail (C-T), submerged Hornwort (H-T), and floating Lemnaminor (L-T). Results showed that the TCS removal efficiencies from water were all greater than 97 %. Maximal TCS adsorption to sediment in the C-T wetland (13.8 ± 0.6 ng/g) was significantly lower than in the H-T wetland (21.0 ± 0.3 ng/g) or the L-T wetland (21.4 ± 0.6 ng/g). The maximal TCS concentrations in plants were 5.7 ± 0.2 and 7.2 ± 0.5 μg/g for H-T and L-T, respectively, and it was below the minimal detection limit (MDL) in C-T. Deep 16S rRNA gene sequencing results revealed that C-T wetland had the highest community richness and diversity. Some bacteria, like beta-Proteobacteria, gamma-Proteobacteria, and Bacteroidetes were detected and might have significant correlations with TCS degradation. Overall, with regard to soils, plants, and microorganism, accumulation in sediment and plants in H-T and L-T was high, while in C-T biodegradation likely played an important role. © 2015, Springer-Verlag Berlin Heidelberg.


Lu J.,Weifang University | Wang S.,Weifang University | Tian J.,Shandong Institute for Product Quality Inspection
Inorganic Chemistry Communications | Year: 2015

Abstract An optically active phthalocyaninato zinc complex containing eight chiral diethyleneglycol mono-(S)-2-methylbutyl ether moieties at the β-position of the phthalocyanine ring, namely (S)-ZnPc (1), was designed, synthesized, and characterized. Using a phase transfer method, the complex 1 was self-assembled to helical nanostructures and the self-assemble behavior was systemically investigated by electronic absorption and circular dichroism spectra, transmission electron microscopy and scanning electron microscopy. In addition, nonlinear optical (NLO) properties of complex 1 and I-V properties of the helical nanostructures formed from complex 1 were also measured. © 2015 Published by Elsevier B.V.


Jia Y.-L.,Ocean University of China | Jia Y.-L.,Shandong Institute for Product Quality Inspection | Wei M.-Y.,Ocean University of China | Wei M.-Y.,Guangdong Medical University | And 4 more authors.
Organic Letters | Year: 2015

A pair of new enantiomeric alkaloid dimers, (+)- and (-)-pestaloxazine A (1), with an unprecedented symmetric spiro[oxazinane-piperazinedione] skeleton, consisting of 22 carbons and 12 heteroatoms, were isolated from a Pestalotiopsis sp. fungus derived from a soft coral. Separation of the enantiomeric alkaloid dimers was achieved by chiral HPLC. Their structures including absolute configurations were elucidated on the basis of a comprehensive analysis of their spectroscopic and X-ray diffraction data and CD calculations. (+)-Pestaloxazine A exhibited potent antiviral activity against EV71 with an IC50 value of 14.2 ± 1.3 μM, which was stronger than that of the positive control ribavirin (IC50 = 256.1 ± 15.1 μM). © 2015 American Chemical Society.


Zhuang H.,Shandong University | Yang M.,Shandong University | Cui Z.,Shandong University | Wang F.,Shandong Institute for Product Quality Inspection
Lecture Notes in Engineering and Computer Science | Year: 2016

Vision based hand gesture recognition is becoming a hot research field. This paper proposes a novel static hand gesture recognition method based on combinational features. The method has two new points. First, it combines the gesture's local binary pattern (LBP) feature with curvature scale space (CSS) corner feature together. Second, it employs the compressive sensing (CS) to classify gestures. Through doing experiments on a hand gesture database confirms that recognition rate can reach 96.25%, it performs better than some other methods.


Li L.,Shandong University | Li L.,Shandong Institute for Product Quality Inspection | Liu E.,Shandong University | Wang X.,Shandong University | And 2 more authors.
Materials Science and Engineering C | Year: 2015

Abstract A polymer film incorporated gold nanoparticle modified electrode was fabricated. The fabricated process involved eletrodeposition of gold nanoparticles and electropolymerization of the 3-methylthiophene (abbreviated 3MT) onto the glassy carbon electrode (GCE). The resulting electrode (P3MT-nano-Au/GCE) was characterized by scanning electron microscopy (SEM), and a simultaneous determination of naphthol isomers at P3MT-nano-Au/GCE was studied using semi-derivative voltammetry. Because of the synergistic effect of gold nanoparticles and poly(3MT), the sensitivity and distinguishability in the simultaneous determination of naphthol isomers were greatly increased. Besides, a further increase in the detecting sensitivity of naphthol isomers could be obtained in the presence of surfactant, cetyl trimethyl ammonium bromide (CTAB). Also, the role of different kinds of surfactants was texted and the action mechanism was discussed in detail. Under the optimal conditions, the linear calibration ranges of the determination of naphthols were 7.0 × 10-7 to 1.5 × 10-4 mol/L for 1-naphthol and 1.0 × 10-6 to 1.5 × 10-4 mol/L for 2-naphthol with detection limits of 1.0 × 10-7 and 3.0 × 10-7 mol/L (S/N = 3), respectively. © 2015 Elsevier B.V. All rights reserved.


PubMed | Shandong University and Shandong Institute for Product Quality Inspection
Type: | Journal: Protein expression and purification | Year: 2016

In well-established heterologous hosts, such as Escherichia coli, recombinant proteins are usually intracellular and frequently found as inclusion bodies-especially proteins possessing high rare codon content. In this study, successful secretory expression of three hydrolases, in a constructed inducible or constitutive system, was achieved by fusion with a novel signal peptide (Kp-SP) from an actinomycete. The signal peptide efficiently enabled extracellular protein secretion and also contributed to the active expression of the intracellular recombinant proteins. The thermophilic -amylase gene of Bacillus licheniformis was fused with Kp-SP. Both recombinants, carrying inducible and constitutive plasmids, showed remarkable increases in extracellular and intracellular amylolytic activity. Amylase activity was observed to be>10-fold in recombinant cultures with the constitutive plasmid, pBSPPc, compared to that in recombinants lacking Kp-SP. Further, the signal peptide enabled efficient secretion of a thermophilic cellulase into the culture medium, as demonstrated by larger halo zones and increased enzymatic activities detected in both constructs from different plasmids. For heterologous proteins with a high proportion of rare codons, it is difficult to obtain high expression in E. coli owing to the codon bias. Here, the fusion of an archaeal homologue of the amylase encoding gene, FSA, with Kp-SP resulted in>5-fold higher extracellular activity. The successful extracellular expression of the amylase indicated that the signal peptide also contributed significantly to its active expression and signified the potential value of this novel and versatile signal peptide in recombinant protein production.

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