Shandong Institute for Food and Drug Control

Jinan, China

Shandong Institute for Food and Drug Control

Jinan, China

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Shi L.,Shandong University | Shi L.,Peking University | Zhang D.,Shandong Institute for Food and Drug Control | Lin R.,Peking University | And 3 more authors.
Tetrahedron Letters | Year: 2014

A novel and efficient transition metal-free C-H bond halogenation of indole derivatives has been developed. 3-Halogenated (3-Br, 3-I) indoles are highly regioselectively produced by this protocol. Simple and readily available halide salts (TBAB, KI) are employed as the halogen source. The transition metal-free and the mild conditions make this protocol very easy to handle and practical. © 2014 Elsevier Ltd. All rights reserved.


Qian Y.,Shandong University | Zhong L.,Shandong Institute for Food and Drug Control | Hou Y.,China Institute of Technology | Qu Y.,Shandong University | Zhong Y.,Shandong University
Frontiers in Microbiology | Year: 2016

The filamentous fungus Trichoderma reesei is a widely used strain for cellulolytic enzyme production. A hypercellulolytic T. reesei variant SN1 was identified in this study and found to be different from the well-known cellulase producers QM9414 and RUT-C30. The cellulose-degrading enzymes of T. reesei SN1 show higher endoglucanase (EG) activity but lower β-glucosidase (BGL) activity than those of the others. A uracil auxotroph strain, SP4, was constructed by pyr4 deletion in SN1 to improve transformation efficiency. The BGL1-encoding gene bgl1 under the control of a modified cbh1 promoter was overexpressed in SP4. A transformant, SPB2, with four additional copies of bgl1 exhibited a 17.1-fold increase in BGL activity and a 30.0% increase in filter paper activity. Saccharification of corncob residues with crude enzyme showed that the glucose yield of SPB2 is 65.0% higher than that of SP4. These results reveal the feasibility of strain improvement through the development of an efficient genetic transformation platform to construct a balanced cellulase system for biomass conversion. © 2016 Qian, Zhong, Hou, Qu and Zhong.


PubMed | Shandong University of Traditional Chinese Medicine, Hospital Pharmacy, Peking University and Shandong Institute for Food and Drug Control
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016

The Chinese herbal formula, Shu-Yu capsule (SYC), has been successfully used to treat depression-like disorders in clinical settings. To rapidly identify the chemical constituents of SYC and its metabolites in rat serum, a simple and sensitive liquid chromatography-tandem mass spectrometry method was established in the present study. By comparing the retention times, MS and MSn spectra data in the literature and reference standards, a total of 73compounds were identified from SYC. In rat serum, 62 components, including 13prototype compounds and 49metabolites were identified. Of these components, 14 metabolites were confirmed as novel metabolites of SYC. The results of the present study indicated that certain avonoid glycosides and monoterpene glycosides were absorbed directly. Glucuronidation and sulfation were identified as the predominant metabolic pathways of the components in SYC. In addition, certain phase I reactions, including hydrolysis, demethylation and hydroxylation occurred in the rats. These results provide scientific evidence, which support further investigations of the pharmacology and mechanism of SYC.


Guo C.,Shandong Institute for Food and Drug Control | Shi F.,Shandong Institute for Food and Drug Control | Gong L.,Shandong Institute for Food and Drug Control | Tan H.,Shandong Institute for Food and Drug Control | And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2015

This paper presents an application of ultrahigh-performance liquid-chromatography - quadrupole - orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) for the ultra-trace analysis of 12 β2-agonists in pork, beef, mutton and chicken meat. The mass spectrometer was operated in Full MS/dd-MS2 (data-dependent MS2) mode, under which a Full MS scan was followed by a dd-MS2 scan with a fragmentation energy. The quantification was achieved using matrix-matched standard calibration curves with salbutamol-d3 and clenbuterol-d9 as the internal standards. The method validation included assessment of selectivity, sensitivity, calibration curve, accuracy, precision, recovery, matrix effect and stability. The results show an exceptional linear relationship with the concentrations of the analytes over wide concentration ranges (e.g., 0.01-50μg/kg for clenbuterol) as all the fitting coefficients of determination r2 are >0.9986. The detection limits (LODs) were in the range of 0.0033-0.01μg/kg, which was much lower than the current reported methods. The recoveries were able to reach 73.0-88.7%, while the matrix effects ranged from 83.7% to 92.8%. Analysis of 400 pork, beef, mutton and chicken samples reveal that only 4.25% samples were positive for β2-agonists. The detected β2-agonists involved salbutamol, clenbuterol, ractopamine and clorprenaline. Overall, the novel Q-Orbitrap technique was demonstrated to have great performance for the screening, identification and quantification of ultra-trace β2-agonists used in food animal muscles, which helps to ensure food safety and public health. © 2015 Elsevier B.V.


Sun P.,Shandong University of Traditional Chinese Medicine | Wei S.,Shandong University of Traditional Chinese Medicine | Wei X.,Shandong Institute for Food and Drug Control | Wang J.,Shandong University of Traditional Chinese Medicine | And 3 more authors.
Neural Plasticity | Year: 2016

Objective. We discuss the influence of anger emotional stress upon VEGF/VEGFR2 and its induced PI3K/AKT/mTOR signal pathway. Methods. We created a rat model of induced anger (anger-out and anger-in) emotional response using social isolation and resident-intruder paradigms and assessed changes in hippocampus' VEGF content, neuroplasticity, and the PI3K/AKT/mTOR signaling pathway. Results. The resident-intruder method successfully generated anger-out and anger-in models that differed significantly in composite aggression score, aggression incubation, open field behavior, sucrose preference, and weight gain. Anger emotional stress decreased synaptic connections and VEGFR2 expression. Anger emotional stress led to abnormal expression of VEGF/VEGFR2 mRNA and protein and disorderly expression of key factors in the PI3K/AKT/mTOR signal pathway. Fluoxetine administration ameliorated behavioral abnormalities and damage to hippocampal neurons caused by anger emotional stress, as well as abnormal expression of some proteins in VEGF/VEGFR2 and its induced PI3K/AKT/mTOR signal pathway. Conclusion. This research provides a detailed classification of anger emotion and verifies its influence upon VEGF and the VEGF-induced signaling pathway, thus providing circumstantial evidence of mechanisms by which anger emotion damages neurogenesis. As VEGFR2 can promote neurogenesis and vasculogenesis in the hippocampus and frontal lobe, these results suggest that anger emotional stress can result in decreased neurogenesis. Copyright © 2016 Peng Sun et al.


Shi F.,Shandong Institute for Food and Drug Control | Guo C.,Shandong Institute for Food and Drug Control | Gong L.,Shandong Institute for Food and Drug Control | Li J.,Shandong Institute for Food and Drug Control | And 6 more authors.
Journal of Chromatography A | Year: 2014

In this study, the applicability of high resolution quadrupole-Orbitrap (Q-Orbitrap) mass spectrometry for the simultaneous qualitative and quantitative analysis of illegal adulterated phosphodiesterase-5 inhibitors (PDE-5 inhibitors) in herbal medicines and dietary supplements was investigated. The mass spectrometer was operated in full MS scan/dd-MS2 (data-dependent MS2) mode. The use of 70000FWHM mass resolution and narrow mass windows (5ppm) could effectively improve the selectivity of the method, increasing the signal-to-noise ratio for the analytes. The response showed good linear relationship with the analytes' concentrations over wide ranges (e.g., 0.05-10μg/g for sildenafil) with all the coefficient of determinations (r2) >0.9996. The detection limits (LODs) were in the range of 1.0-5.0ng/g for different analytes. The recoveries ranged from 85.4% to 96.7%. The intra- and inter-day accuracies were in the range of -6.6 to 10.1%, while the intra- and inter-day precision ranged from 0.0039% to 13.2%. Among 68 batches of herbal medicines and 20 batches of dietary supplements (including 83 capsules, 3 pellets and 2 liquid) samples, sildenafil was detected in 8 dietary supplements, while noracetildenafil was detected in only one dietary supplement. The novel Q-Orbitrap mass spectrometry has been proved to be a very promising and powerful tool for routine screening of illegal adulterate in herbal medicines and dietary supplements, ensuring food safety and public health. © 2013 Elsevier B.V.


Miao T.,Shandong University | Li Y.,Shandong University | Zhang D.,Shandong Institute for Food and Drug Control
Journal of Nanoscience and Nanotechnology | Year: 2016

We report the successful synthesis of ultralong polyaniline nanorods (UL-PANI-NRs) via using watersoluble magnetic Fe3O4 nanoparticles as soft templates under the assistance of external magnetic field. It was found that the concentration of Fe3O4 nanoparticles, the aniline concentration and the use of an external magnetic field significantly affect the morphology of the PANI products. The following characterizations including transmission electron microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA) were conducted to investigate the electronic structure and composition of as-prepared UL-PANI-NRs. Our preliminary result indicates that complicated polymer structures (such as ultralong rods with vertical branches) may be prepared by water-soluble magnetic Fe3O4 nanoparticles as soft templates under the assistance of alternatively external magnetic fields. Copyright © 2016 American Scientific Publishers All rights reserved.


PubMed | Shandong University of Traditional Chinese Medicine and Shandong Institute for Food and Drug Control
Type: | Journal: Neural plasticity | Year: 2016

Objective. We discuss the influence of anger emotional stress upon VEGF/VEGFR2 and its induced PI3K/AKT/mTOR signal pathway. Methods. We created a rat model of induced anger (anger-out and anger-in) emotional response using social isolation and resident-intruder paradigms and assessed changes in hippocampus VEGF content, neuroplasticity, and the PI3K/AKT/mTOR signaling pathway. Results. The resident-intruder method successfully generated anger-out and anger-in models that differed significantly in composite aggression score, aggression incubation, open field behavior, sucrose preference, and weight gain. Anger emotional stress decreased synaptic connections and VEGFR2 expression. Anger emotional stress led to abnormal expression of VEGF/VEGFR2 mRNA and protein and disorderly expression of key factors in the PI3K/AKT/mTOR signal pathway. Fluoxetine administration ameliorated behavioral abnormalities and damage to hippocampal neurons caused by anger emotional stress, as well as abnormal expression of some proteins in VEGF/VEGFR2 and its induced PI3K/AKT/mTOR signal pathway. Conclusion. This research provides a detailed classification of anger emotion and verifies its influence upon VEGF and the VEGF-induced signaling pathway, thus providing circumstantial evidence of mechanisms by which anger emotion damages neurogenesis. As VEGFR2 can promote neurogenesis and vasculogenesis in the hippocampus and frontal lobe, these results suggest that anger emotional stress can result in decreased neurogenesis.


PubMed | Shandong University and Shandong Institute for Food and Drug Control
Type: | Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences | Year: 2016

In this work, the ultrahigh-performance liquid chromatography quadrupole orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) was applied to the rapid screening, identification and quantification of the illegal adulterated glucocorticoids in herbal medicines. The mass spectrometer was operated in positive ion mode and Full MS/dd-MS


PubMed | Shandong Institute for Food and Drug Control and Zhejiang University
Type: Journal Article | Journal: Applied biochemistry and biotechnology | Year: 2016

S-Adenosyl-L-methionine (SAM) plays important roles in trans-methylation, trans-sulfuration, and polyamine synthesis in all living cells, and it is also an effective cure for liver disease, depressive syndromes, and osteoarthritis. The increased demands of SAM in pharmaceuticals industry have aroused lots of attempts to improve its production. In this study, a multiple-copy integrative plasmid pYMIKP-SAM2 was introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001. Further studies showed that the recombinant yeast exhibited higher enzymatic activity of methionine adenosyltransferase and improved its SAM biosynthesis. With a three-phase fed-batch strategy in 15-liter bench-top fermentor, 8.81 g/L SAM was achieved after 52 h cultivation of R1-ZJU001, about 27.1 % increase over its parent strain ZJU001, whereas the SAM content was also improved from 64.6 mg/g DCW to 91.0 mg/g DCW. Our results shall provide insights into the metabolic engineering of SAM pathway in yeast for improved productivity of SAM and subsequent industrial applications.

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