Shandong Fuyang Biological Technology Co.

Fuyang, China

Shandong Fuyang Biological Technology Co.

Fuyang, China
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Lu F.,East China University of Science and Technology | Li C.,East China University of Science and Technology | Wang Z.,East China University of Science and Technology | Zhao W.,Shandong Fuyang Biological Technology Co. | And 3 more authors.
Bioresource Technology | Year: 2016

In this paper, a system of cell-recycle continuous fermentation for sodium gluconate (SG) production by Aspergillus niger (A. niger) was established. Based on initial continuous fermentation result (100.0 h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5 h). Due to less auxiliary time, highest SG production rate (31.05 ± 0.29 g L−1 h−1) and highest yield (0.984 ± 0.067 mol mol−1), overall SG production capacity (975.8 ± 5.8 g h−1) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved. © 2016 Elsevier Ltd


Lu F.,East China University of Science and Technology | Wang Z.,East China University of Science and Technology | Zhao W.,Shandong Fuyang Biological Technology Co. | Chu J.,East China University of Science and Technology | Zhuang Y.,East China University of Science and Technology
Bioresource Technology | Year: 2016

In this paper, approach for real-time monitoring of sodium gluconate (SG) fermentation was established for the first time by the equations which can calculate real-time key-parameters by on-line physiological data. Based on this approach, limiting factors were found out in initial fermentation F1 and then step-wise agitation increase and improved medium recipe were proposed in fermentation F2 and F3, respectively. The highest average SG production rate (16.58±0.91gL-1h-1) was achieved in fermentation F3, which was 104.2% and 48.0% higher than those in fermentation F1 and F2, respectively. Meanwhile, due to shorter fermentation period (decreased from 34h to 18.7h), lower biomass (about 1.5gL-1) and less by-product accumulation, the overall yield of 0.943±0.012 (molmol-1) in fermentation F3 increased more than 16.0% compared to fermentation F1. This approach had been successfully applied to industrial fermentation and greatly improved SG production. © 2015 Elsevier Ltd.


Lu F.,East China University of Science and Technology | Ping K.,East China University of Science and Technology | Wen L.,East China University of Science and Technology | Zhao W.,Shandong Fuyang Biological Technology Co. | And 3 more authors.
Process Biochemistry | Year: 2015

Effects of seed morphology of Aspergillus niger (A. niger) AN151 on gluconic acid (GA) biosynthesis were investigated in 50-L fermenters. Online physiological parameters showed that the optimum seed morphology for GA biosynthesis was dispersed pattern rather than pellets which were widely used in industrial production. The highest overall yield of 1.051±0.012 (gg-1) was achieved using dispersed seed in shorter fermentation time (14.5h) with an initial glucose solution of 330gL-1, and was 3.1% higher than the best reported data. And the average GA production rate under dispersed fermentation (21.0±0.9gL-1 h-1) was 73.6% and 39.1% higher than those with large and small pellets, respectively. The fermentation results showed that conversion of mycelial morphology from pellets to dispersed state had negligible impact on viscosity of fermentation broth at low biomass concentration (1.7gL-1), while coefficient of volumetric oxygen transfer (kLa) improved more than 13%, suggesting that the promotion of oxygen transfer rate (OTR) between liquid medium and mycelia greatly increased GA production. Therefore, controlling the morphology of A. niger is an effective method for improving industrial GA production. © 2015 Elsevier Ltd.


Ping K.-K.,East China University of Science and Technology | Wang Z.-J.,East China University of Science and Technology | Lu F.,East China University of Science and Technology | Zhao W.,Shandong Fuyang Biological Technology Co. | And 3 more authors.
Journal of Chemical Technology and Biotechnology | Year: 2015

BACKGROUND: In this study, the performance of aerobic batch fermentation with Aspergillus niger producing sodium gluconate under different oxygen supply levels through adjusting the agitation rate were investigated. The response of glucose metabolism in A. niger to different oxygen uptake rate (OUR) levels has been studied in the present work. RESULTS: Metabolic flux analysis demonstrated that the high oxygen supply condition was favorable for cell growth and initial sodium gluconate synthesis during the early fermentation phases. However, during the late stable phase, metabolic flux analysis indicated that a high yield of sodium gluconate production could be achieved at a medium OUR level of 55±2.5mmolL-1h-1 as less flux was required for glucolysis and the TCA cycle. With a two-stage OUR control strategy, the final sodium gluconate yield of the batch fermentation was enhanced and reached 93.7% (mol vs mol), which was higher than those obtained using a high oxygen supply level throughout the whole fermentation process. CONCLUSIONS: Metabolic flux analysis was successfully used in the present work, and the two-stage OUR control strategy increased the yield of sodium gluconate production to 93.7%. © 2015 Society of Chemical Industry.


PubMed | East China University of Science and Technology and Shandong Fuyang Biological Technology Co.
Type: | Journal: Bioresource technology | Year: 2016

In this paper, approach for real-time monitoring of sodium gluconate (SG) fermentation was established for the first time by the equations which can calculate real-time key-parameters by on-line physiological data. Based on this approach, limiting factors were found out in initial fermentation F1 and then step-wise agitation increase and improved medium recipe were proposed in fermentation F2 and F3, respectively. The highest average SG production rate (16.580.91 g L(-1) h(-1)) was achieved in fermentation F3, which was 104.2% and 48.0% higher than those in fermentation F1 and F2, respectively. Meanwhile, due to shorter fermentation period (decreased from 34 h to 18.7 h), lower biomass (about 1.5 g L(-1)) and less by-product accumulation, the overall yield of 0.9430.012 (mol mol(-1)) in fermentation F3 increased more than 16.0% compared to fermentation F1. This approach had been successfully applied to industrial fermentation and greatly improved SG production.


PubMed | East China University of Science and Technology and Shandong Fuyang Biological Technology Co.
Type: | Journal: Bioresource technology | Year: 2016

In this paper, a system of cell-recycle continuous fermentation for sodium gluconate (SG) production by Aspergillus niger (A. niger) was established. Based on initial continuous fermentation result (100.0h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5h). Due to less auxiliary time, highest SG production rate (31.050.29gL(-1)h(-1)) and highest yield (0.9840.067molmol(-1)), overall SG production capacity (975.85.8gh(-1)) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved.

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