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Li G.-S.,Yantai University | Jiang W.-L.,Yantai University | Jiang W.-L.,Shandong Engineering Research Center for Nature Drug | Tian J.-W.,Yantai University | And 3 more authors.

This study was carried out to investigate whether rosmarinic acid (RA) has antifibrotic effect on experimental liver fibrosis in vitro and in vivo and its possible mechanism. Culture of hepatic stellate cells (HSCs) determine proliferation and expression of transforming growth factor-β1 (TGF-β1), connective transforming growth factor (CTGF) and α-smooth muscle actin (α-SMA). In carbon tetrachloride (CCL4)-induced rat liver fibrosis model, determined biochemical indicator, liver fibrosis grade and histopathological changes, immunohistochemical detected liver TGF-β1 and CTGF expression. The results indicated that RA could inhibit HSCs proliferation, inhibit TGF-β1, CTGF and α-SMA expression in cultured HSCs. It has marked evident in reducing fibrosis grade, ameliorating biochemical indicator and histopathological morphology, reducing liver TGF-β1 and CTGF expression in CCL4-induced liver fibrosis. These findings suggest that RA has potentially conferring antifibrogenic effects. © 2009 Elsevier GmbH. All rights reserved. Source

Jiang W.-L.,Yantai University | Jiang W.-L.,Shandong Engineering Research Center for Nature Drug | Fu F.-H.,Yantai University | Xu B.-M.,Yantai University | And 3 more authors.

The present study was undertaken to examine the effect of forsythoside B (FB) on rat myocardial ischemia-reperfusion (I/R) model and elucidate the potential mechanism. Left ventricular systolic pressure (LVSP) and ±dp/dtmax were detected. Blood samples were collected to determine serum levels of troponin T (Tn-T), TNF-α and IL-6. Hearts were harvested to assess histopathological change and infarct size, determine content of MDA, myeloperoxidase (MPO), SOD and GPx activities, analyze expression of high-mobility group box 1 (HMGB1), phosphor-IκB-α and phosphor-nuclear factor kappaB (NF-κB) in ischemic myocardial tissue by Western blot. Compared with control group, rats treatment with FB showed a significant recovery in myocardial function with improvement of LVSP and ±dp/dtmax. The myocardial infarct volume, serum levels of Tn-T, TNF-α and IL-6, content of MDA and MPO activity in myocardial tissue were all reduced, protein expression of HMGB1, phosphor-IκB-α and phosphor-NF-κB were down-regulated, while attenuated the decrease of SOD and GPx activities. Besides, the infiltration of polymorph nuclear leukocytes (PMNs) and histopathological damages in myocardium were decreased in FB treated groups. These findings suggested that FB rescued cardiac function from I/R injury by limiting inflammation response and its antioxidant properties. © 2009 Elsevier GmbH. All rights reserved. Source

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