Shandong Drug and Food Vocational College

Weihai, China

Shandong Drug and Food Vocational College

Weihai, China
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Wang Y.-H.,Shandong Drug and Food Vocational College | Li B.,Shandong Institute for Food and Drug Control | Chen Z.,Shandong Institute for Food and Drug Control
Chinese Pharmaceutical Journal | Year: 2014

OBJECTIVE: To establish a method for simultaneous determination of seven antimicrobial agents (sulfadiazine, pipe-midic acid, trimethoprim, sulfamethazine, ofloxacin, norfloxacin and sulfamethoxazole) in adulterated Chinese patent medicines for relieving cough and asthma. METHODS: The analysis was performed on a Venusil MP C18 column (4.6 mm × 250 mm, 5 μm) with a gradient elution (0-10 min, 10% A; 10-40min, 10% A → 40% A) of acetonitrile-methanol(1:1) (A)-0.3% ammonium formate solution containing 0.1% formic acid (B) with a flow rate of 1 mL · min-1. The detection wavelength was 280 nm, reference wavelength was 360 nm, and the scanning range was from 190 to 400 nm. The column temperature was 30°C. RESULTS: Sulfadiazine, pipemidic acid, trimethoprim, sulfamethazine, ofloxacin, norfloxacin and sulfamethoxazole had good linearities in the range of 1.4-18.0 μg · mL-1(r=1.0000), 1.4-17.6 μg · mL-1 (r=0.9997), 2.4-30.0 μg · mL-1 (r=0.9998), 1.5-19.2 μg · mL-1 (r=1.0000), 2.6-32.8 μg · mL-1 (r=0.9996), 2.0-24.4 μg · mL-1 (r=1.0000) and 1.4-17.6 μg · mL-1 (r=1.0000), respectively. The recoveries of high, median, and low concentrations control samples were 94.5%-100.6%, 90.3%-98.3%, 93.5%-98.0%, 95.1%-101.7%, 92.8%-103.1%, 93.2%-100.9% and 95.5%-106.3%, respectively. The test result of three batches of negative samples was negative, and the test result of three batches of analog positive samples was positive. Trimethoprim and sulfamethoxazole were found in three batches of counterfeit drugs. CONCLUSION: The method can be used for examining seven antimicrobial agents in adulterated Chinese patent medicines.


Chen Y.-C.,China Pharmaceutical University | Chen Y.-C.,Fudan University | Cao W.-W.,China Pharmaceutical University | Cao W.-W.,Southern Medical University | And 9 more authors.
Journal of Ethnopharmacology | Year: 2011

Ethnopharmacological relevance: Danshensu is an active water-soluble component from Salvia Miltiorrhiza, which has been demonstrated holding multiple mechanisms for the regulation of cardiovascular system. However, the relative contribution of danshensu to its multiple cardiovascular activities remains largely unknown. Aim of the study: To develop an artificial neural network (NN) model simultaneously characterizing danshensu pharmacokinetics and multiple cardiovascular activities in acute myocardial infarction (AMI) rats. The relationship between danshensu pharmacokinetics (PK) and pharmacodynamics (PD) were evaluated using contribution values. Materials and methods: Danshensu was intraperitoneally injected at a single dose of 20 mg/kg to AMI rats induced by coronary artery ligation. Plasma levels of danshensu, cardiac troponin T (cTnT), total homocysteine (Hcy) and reduced glutathione (GSH) were quantified. A back-propagation NN model was developed to characterize the PK and PD profiles of danshensu, in which the input variables contained time, area under plasma concentration-time curve (AUC) of danshensu and rat weights (covariate). Relative contribution of input variable to the output neurons was evaluated using neuron connection weights according to Garson's algorithm. The kinetics of contribution values was also compared and was validated using bootstrap resampling method. Results: Danshensu exerted significant cTnT-lowering, Hcy- and GSH-elevating effect, and these marker profiles were well captured by the trained NN model. The calculation of relative contributions revealed that the effect of danshensu on the PD marker could be ranked as cTnT > GSH > Hcy, while the effect of AMI disease on the PD marker could be ranked in the following order: cTnT > Hcy > GSH. The activity of transsulfuration pathway was quite obvious under the AMI state. Conclusion: NN is a powerful tool linking PK and PD profiles of danshensu with multiple cardioprotective mechanisms, it provides a simple method for identifying and ranking relative contribution to the multiple therapeutic effects of the drug. © 2011 Elsevier Ireland Ltd.


Song H.-Z.,Hefei University of Technology | Bi L.,Shandong Drug and Food Vocational College | Lv S.,Hefei University of Technology | Lu J.-F.,Hefei University of Technology | And 3 more authors.
Modern Food Science and Technology | Year: 2014

Fish skin is the main byproduct in channel catfish filet processing industrial. Preparation antihypertensive peptides using channel catfish skin gelatin can promote its utility value. Spontaneously hypertensive rats (SHR) and normal SD rats were used to assess the antihypertensive effects of the ACE inhibitory peptides under optimized condition in vivo. The ACE activity and Ang II contents in the serum and lung tissue of SHR were measured. The blood pressure of SHR was decreased significantly after one-time dosing experiment and long-term dosing study (28 days), and the antihypertensive effects were dose-dependent. The blood pressure of SHR in high dose group decreased from 206 mmHg to 159 mmHg after 2 h oral administration and remained around 155 mmHg after long-term oral administration for 10 days. Moreover, the ACE inhibitory peptides had no significant effect on normal SD rats. The ACE inhibitory peptide from channel catfish skin gelatin inhibited the ACE activity, and decreased the Ang II contents in serum and lung tissue of SHR. While there was no significant effect on ACE activity and Ang II content in serum and hung tissue for normal SD rats.


Zhao J.F.,Shandong Drug and Food Vocational College
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To identify consistency of the composition and efficacy of Porcellio scaber and Armadillidium vulgare and provide reference for the safe and scientific use of the medicinal material. Identified the Porcellio scaber and the Armadillidium vulgare with the method of characteristics; Compared the two chemical composition by thin layer method; Determined the extract by water and ethanol using the pharmacopoeia extract method. Porcellio scaber and Armadillidium vulgare were two varieties, they had similar chemical composition, the water and ethanol extract were the same. The compostion and efficary of Porcellio scaber and Armadillidium vulgare are the same, and these two varieties can be mixed or used alone.


Wan M.,Shandong University | Xu L.,Shandong University | Hua L.,Shandong Drug and Food Vocational College | Li A.,Shandong Drug and Food Vocational College | And 6 more authors.
Bioorganic Chemistry | Year: 2014

A series of novel isoxazolyl chalcones were synthesized and evaluated for their activities in vitro against four types of human non-small cell lung cancer cells, including H1792, H157, A549 and Calu-1 cells. The preliminary biological screening showed that compounds 5d and 5f-i exhibited significant cytotoxicity, particularly, compounds 5f and 5h were identified as the most potent anticancer agents with IC50 values 1.35-2.07 μM and 7.27-11.07 μM against H175, A549 and Calu-1 cell lines, respectively. Compounds 5f-i could induce apoptosis in A549 cells by death receptor 5 (DR5) mediated extrinsic pathways. The preliminary structure-activity relationship study showed that compounds bearing electron withdrawing groups (EWG) at the 2-position of the phenyl ring in Ar group were more effective than those with EWG at 4-position. These results further demonstrated that the scaffolds designed in this work might lead to the discovery of novel anti-lung cancer agents. © 2014 Elsevier Inc. All rights reserved.


Tan W.,CAS Yantai Institute of Coastal Zone Research | Tan W.,University of Chinese Academy of Sciences | Li Q.,CAS Yantai Institute of Coastal Zone Research | Wang H.,Shandong Drug and Food Vocational College | And 5 more authors.
Carbohydrate Polymers | Year: 2016

Four novel starch-linked-1,2,3-triazole derivatives were synthesized including 6-hydroxymethyltriazole-6-deoxy starch (HMTST), 6-bromomethyltriazole-6-deoxy starch (BMTST), 6-chloromethyltriazole-6-deoxy starch (CMTST), and 6-carboxyltriazole-6-deoxy starch (CBTST). Their antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were evaluated in vitro, respectively. The inhibitory property of the obtained amphiprotic starch derivatives exhibited a remarkable improvement over starch. And the antibacterial indices of most of the products were higher than 60% and 40% at 1.0 mg/mL when the culture time was 8 h and 16 h, respectively. Moreover, the inhibitory index of CBTST attained 97% above at 1.0 mg/mL. Generally, the inhibitory activity decreased in the order: CBTST > CMTST > BMTST > HMTST > starch. Furthermore, the order of their antibacterial activity was consistent with the electron-withdrawing property of different substituted groups of the 1,2,3-triazole groups. The substituted groups with stronger electron withdrawing ability relatively possessed greater antibacterial activity. © 2016 Elsevier Ltd. All rights reserved.


Hu Z.-G.,Central South University | Hu Z.-G.,Guilin University of Technology | Hu Z.-G.,Chinalco Guangxi Nonferrous Rare Earth Development Co. | Zhao R.-X.,Guilin University of Technology | And 3 more authors.
Journal of Cluster Science | Year: 2016

A novel heterometallic heptanuclear cluster [(hmbame)2(Formula presented.)CoIINa2(N3)8 (OH)2]·4H2O (1), (H3hmbame is 6,6′-((1Z,1′E)-(((3-ethoxy-2-hydroxyphenyl) methylene)bis(azanylylidene))bis(methanylylidene))bis (2-ethoxy phenol)) was synthesized by in situ reaction. Complex 1 was structurally characterized by elemental analysis, IR, UV–Vis spectroscopy and single crystal X-ray diffraction. Complex 1 crystallizes in the triclinic space group Pī, showing a disc-like configuration. Luminescent property of 1 has been studied. The results indicate that it may be good candidate for useful photoactive material. © 2016 Springer Science+Business Media New York


Li S.-M.,Shandong Drug and Food Vocational College | Yang J.-L.,Shandong Drug and Food Vocational College | Liu Y.-P.,Shandong Drug and Food Vocational College | Liu Y.-P.,Hainan Normal University | Fu Y.-H.,Hainan Normal University
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: To study the non-alkaloid constituents from the stems and leaves of Alstonia mairei. Methods: The chemical constituents were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results: Eighteen compounds were isolated from the petroleum ether fraction of 90% ethanol extract from the stems and leaves of A. mairei, and identified as lupeol (1), 30-oxo-lupeol (2), lupenyl acetate (3), α-amyrin (4), α-amyrenone (5), 23-hydroxyursolic acid (6), β-amyrin (7), β-amyrenone (8), maslinic acid (9), friedelinol (10), friedelin (11), tectochrysin (12), 5,6-dihydroxy-7,4'- dimethoxyflavone (13), 5,3'-dihydroxy-7,4'-dimethoxyflavone (14), 7-hydroxy-7,5,3',4'-trimethoxyflavone (15), 5,7,3',4'- tetramethoxyflavone (16), 5-hydroxy-6,7,8,4-tetramethoxyflavone (17), and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (18). Conclusion: All compounds are isolated from A. mairei for the first time. In addition to compound 1, the other compounds are isolated from the plants of Alstonia R. Br. for the first time. © 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


PubMed | CAS Yantai Institute of Coastal Zone Research and Shandong Drug and Food Vocational College
Type: | Journal: Carbohydrate polymers | Year: 2016

Four novel starch-linked-1,2,3-triazole derivatives were synthesized including 6-hydroxymethyltriazole-6-deoxy starch (HMTST), 6-bromomethyltriazole-6-deoxy starch (BMTST), 6-chloromethyltriazole-6-deoxy starch (CMTST), and 6-carboxyltriazole-6-deoxy starch (CBTST). Their antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were evaluated in vitro, respectively. The inhibitory property of the obtained amphiprotic starch derivatives exhibited a remarkable improvement over starch. And the antibacterial indices of most of the products were higher than 60% and 40% at 1.0 mg/mL when the culture time was 8 h and 16 h, respectively. Moreover, the inhibitory index of CBTST attained 97% above at 1.0 mg/mL. Generally, the inhibitory activity decreased in the order: CBTST>CMTST>BMTST>HMTST>starch. Furthermore, the order of their antibacterial activity was consistent with the electron-withdrawing property of different substituted groups of the 1,2,3-triazole groups. The substituted groups with stronger electron withdrawing ability relatively possessed greater antibacterial activity.


PubMed | Shandong Drug and Food Vocational College, Shandong University and Shandong Normal University
Type: | Journal: Bioorganic chemistry | Year: 2014

A series of novel isoxazolyl chalcones were synthesized and evaluated for their activities in vitro against four types of human non-small cell lung cancer cells, including H1792, H157, A549 and Calu-1 cells. The preliminary biological screening showed that compounds 5d and 5f-i exhibited significant cytotoxicity, particularly, compounds 5f and 5h were identified as the most potent anticancer agents with IC50 values 1.35-2.07 M and 7.27-11.07 M against H175, A549 and Calu-1 cell lines, respectively. Compounds 5f-i could induce apoptosis in A549 cells by death receptor 5 (DR5) mediated extrinsicpathways. The preliminary structure-activity relationship study showed that compounds bearing electron withdrawing groups (EWG) at the 2-position of the phenyl ring in Ar group were more effective than those with EWG at 4-position. These results further demonstrated that the scaffolds designed in this work might lead to the discovery of novel anti-lung cancer agents.

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