Shandong Dong E E Jiao Co.

Donge, China

Shandong Dong E E Jiao Co.

Donge, China
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Wei M.,CAS Institute of Botany | Lu Y.,CAS Institute of Botany | Liu D.,Natural Herbal Farm Inc. | Ru W.,Shandong Dong E E Jiao Co.
Biological and Pharmaceutical Bulletin | Year: 2014

Catalpol, an iridoid glycoside obtained from various natural sources, has many biological functions. However, its ovarian failure-resistant effect has scarcely been studied. The present study used senile 14-month-old Sprague-Dawley female rats to examine the in vivo ovarian failure-resistant activity of catalpol. Daily oral graded doses of catalpol (1, 3, or 5 mg/kg/d) for 4 weeks significantly increased the levels of serum 17β-estradiol (E2) and progesterone (P4) but reduced follicle-stimulating hormone and luteinizing hormone levels. Electron microscopic analysis and flow cytometry showed that catalpol significantly retarded apoptosis of the ovarian granulocytes of the rats. These findings suggest that catalpol works on the sex organs by nourishing ovarian tissues and improving both the quality and quantity of follicles, thus leading to rebalanced E2and P4 levels in aged rats so that catalpol has a direct in vivo antiaging effect on the rat ovarian system. © 2014 The Pharmaceutical Society of Japan.


Cheng X.-L.,National Institutes for Food and Drug Control | Cheng X.-L.,Beijing University of Chinese Medicine | Wei F.,National Institutes for Food and Drug Control | Xiao X.-Y.,National Institutes for Food and Drug Control | And 8 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC/Q-TOF-MS) method coupled with a principal component analysis (PCA) was developed and applied toward identifying donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, tortoise shell glue, and deerhorn glue. The UPLC-MS data of the trypsin digested samples were subjected to principal component analysis (PCA) in order to classify these five gelatins. Additionally, marker peptides given by the loadings plot of PCA were identified based on a comparison of recorded LC-MS data with a previously reported database of the corresponding gelatin variants. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of gelatins commonly used in Traditional Chinese Medicine (TCM). © 2011 Elsevier B.V.


Han H.,Zhejiang University | Zhang L.,Shandong Dong E E Jiao Co. | Zhang Y.,Shandong Dong E E Jiao Co. | Li W.,Zhejiang University | Qu H.,Zhejiang University
Analytical Methods | Year: 2013

A rapid and simultaneous quantification of the main active compounds of the in-process extract solutions is beneficial to the process monitoring as well as ensuring quality consistency of the end products during the production of traditional Chinese medicine. Here we present a near infrared (NIR) spectroscopy-based method for rapid analysis of extract solutions in the production of compound E Jiao oral liquid. The partial least-squares regression (PLSR) models for four quality indicators (viz. total flavonoids, total saponins, total saccharides and soluble solid contents) were established and validated. The results showed that all of the four models exhibited satisfactory fitting and predictive capacity. The root mean squares error of prediction (RMSEP) was 0.0384 mg mL-1, 0.0154 mg mL-1, 3.80 mg mL-1 and 0.199% for total flavonoids, total saponins, total saccharides and soluble solid contents, respectively. This work here demonstrated that NIR spectroscopy coupled with PLSR calibration can offer a reliable and non-destructive alternative in the routine monitoring of the extraction process in the production of compound E Jiao oral liquid. The presented approach is expected to be equally applicable to the mixed decoction of other herbal medicines. © 2013 The Royal Society of Chemistry.


Wang S.,Zhejiang University | Zheng G.,Zhejiang University | Tian S.,Shandong Dongeejiao Co. | Zhang Y.,Shandong Dongeejiao Co. | And 4 more authors.
Life Sciences | Year: 2015

Aims We aimed to investigate the effects of echinacoside (ECH) on hematopoietic function in 5-FU-induced bone marrow depression mice. Main methods In vitro, after stimulation with ECH, the proliferation ability of bone marrow (BM) cells and bone marrow stromal cells (BMSCs) derived from myelosuppression mice were assessed by CCK8 assay and morphology, respectively. In vivo, 5-FU-induced myelosuppression or control mice were intragastrically administrated with either ECH at 15 mg/kg or the equal volume of normal saline daily for 12 days before BM cells were isolated for colony-forming cell assay. Meanwhile, BMSCs were cultured for 4 weeks before cells were observed for growth pattern, cell culture supernatants were collected for GM-CSF secretion by ELISA, and RNA of the cells were extracted for EPO and GM-CSF RT-PCR. BM cells or BMSCs stimulated with ECH for 24 h or 48 h were collected for protein extraction and Western blotting. Key findings ECH stimulated the growth of BM cells but not BMSCs derived from 5-FU treated mice. The intragastric administration of ECH in 5-FU treated mice could increase the number of total hematopoietic progenitor cells and GM progenitor cells to healthy control mice level, but not BFU progenitor cells. BMSCs from ECH treated myelosuppression mice grew more vigorously and expressed more GM-CSF, but not EPO. ECH activated the PI3K signaling pathway in 5-FU suppressed BM cells. Significance ECH could improve the hematopoietic function of bone marrow in 5-FU-induced myelosuppression mice. ECH can be considered as an alternative effective therapy for patients during chemotherapy or HSC transplantation. © 2015 Elsevier Inc. All rights reserved.


PubMed | Shandong Donge Ejiao Co. and Zhejiang University
Type: Journal Article | Journal: Journal of Zhejiang University. Science. B | Year: 2016

We have developed a set of chemometric methods to address two critical issues in quality control of a precious traditional Chinese medicine (TCM), Donge Ejiao (DEEJ). Based on near infrared (NIR) spectra of multiple samples, the genuine manufacturer of DEEJ, e.g. Donge Ejiao Co., Ltd., was accurately identified among 21 suppliers by the fingerprint method using Hotelling T(2), distance to Model X (DModX), and similarity match value (SMV) as discriminate criteria. Soft independent modeling of the class analogy algorithm led to a misjudgment ratio of 6.2%, suggesting that the fingerprint method is more suitable for manufacturer identification. For another important feature related to clinical efficacy of DEEJ, storage time, the partial least squares-discriminant analysis (PLS-DA) method was applied with a satisfactory misjudgment ratio (15.6%) and individual prediction error around 1 year. Our results demonstrate that NIR spectra comprehensively reflect the essential quality information of DEEJ, and with the aid of proper chemometric algorithms, it is able to identify genuine manufacturer and determine accurate storage time. The overall results indicate the promising potential of NIR spectroscopy as an effective quality control tool for DEEJ and other precious TCM products.


Liu M.,Shandong University | Tan H.,Shandong University | Zhang X.,Shandong University | Liu Z.,Shandong University | And 4 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Fufang Ejiao Jiang (FEJ), which has been widely used in clinic to replenish qi (vital energy) and nourish blood, is a famous traditional Chinese medicine formula made up of Colla corii Asini (Donkey-hide gelatin prepared by stewing and concentrating from the hide of Equus asinus Linnaeus.), Radix Codonopsis Pilosulae (the root of Codonopsis pilosula (Franch.) Nannf.), Radix Ginseng Rubra (the steamed and dried root of Panax ginseng C.A. Mey.), Fructus Crataegi (the fruit of Crataegus pinnatifida Bunge) and Radix Rehmanniae Preparata (the steamed and sun dried tuber of Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch. & C.A. Mey.). The present study aimed to investigate the hematopoietic effects of FEJ on myelosuppressed mice induced by radiotherapy and chemotherapy systematically and to explore the underlying hematopoietic regulation mechanisms. Methods The myelosuppressed mouse model was induced by 60Co radiation, cyclophosphamide and chloramphenicol. FEJ was then administered by i.g. at the dosages of 5, 10, or 20 mL/kg·d for 10 d. The numbers of blood cells from peripheral blood and bone marrow nucleated cells (BMNC) were counted. Body weight and the thymus and spleen indices were also measured. The numbers of hemopoietic progenitor cells and colony-forming unit-fibroblast (CFU-F) were measured in vitro. The ratio of hematopoietic stem cells (HSC) in BMNC, cell cycle and apoptosis of BMNC were determined by flow cytometry. The histology of femoral bone was examined by H&E staining. The levels of transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α), erythropoietin (EPO), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and interleukin-6 (IL-6) in serum were measured by ELISA. IL-1β, IL-3, IL-6 mRNA levels in spleen were detected by real-time quantitative PCR (RT-qPCR). In addition, bone marrow stromal cells (BMSC) were cultured in vitro followed by treatment with different doses of FEJ (2.5, 5, 10 μL/mL) for 48 h. Then the levels of cytokines (IL-6, SCF, GM-CSF) in the conditioned media and their mRNA levels in BMSC were determined by ELISA and RT-qPCR, respectively. Results FEJ could significantly increase the numbers of peripheral blood cells and BMNC, and reverse the loss of body weight and the atrophy of thymus and spleen in a dose-dependent manner. The quantities of hemopoietic progenitor cells and CFU-F in bone marrow were also significantly increased in a dose-dependent manner after FEJ administration. A high-dose FEJ of 20 mL/kg·d could significantly increase the ratio of HSC in BMNC, promote bone marrow cells entering the proliferative cycle phase (S+G2/M) and prevent cells from proceeding to the apoptotic phase. FEJ could also improve the femoral bone marrow morphology. Furthermore, FEJ could increase the levels of GM-CSF and IL-3 and reduce the level of TGF-β in serum, and enhance the expressions of IL-1β and IL-3 mRNA in spleen. Lastly, the levels of cytokines (IL-6, SCF, GM-CSF) in the conditioned media and their mRNA levels in BMSC were elevated after treatment with FEJ. Conclusions FEJ was clearly confirmed to promote the recovery of bone marrow hemopoietic function in a myelosuppressed mouse model, which may be attributed to (i) improving bone marrow hematopoietic microenvironment; (ii) facilitating the cell proliferation and preventing BMNC from apoptosis; (iii) stimulating the expressions of IL-1β, IL-3, IL-6, SCF and GM-CSF and inhibiting the expression of TGF-β. © 2014 Elsevier Ireland Ltd.


PubMed | East China University of Science and Technology and Shandong Dong e E Jiao Co.
Type: Journal Article | Journal: Biotechnology and applied biochemistry | Year: 2016

Insulin precursor fusion protein expressed in Pichia pastoris is a single-chain protein with a spacer peptide (EEAEAEAEPK) localized at its N-terminal. Currently, the one-step transpeptidation reaction with low yield and high cost is generally employed to convert the insulin precursor fusion protein into human insulin ester. In this study, a two-step transpeptidation reaction was proposed separating the cleavage step from the coupling step so that each reaction was performed under its optimal conditions. Using this method, the total efficiency doubled and the reaction time was shortened compared with the one-step method. In addition, the amount of O-t-butyl-l-threonine t-butyl ester and trypsin dosages were reduced by 50% and 75%, respectively. This two-step transpeptidation strategy was simple and efficient and could be used for the pharmaceutical production of human insulin.


An active small-molecule donkey-hide gelatin mixture and a preparation method and application thereof. The active small-molecule donkey-hide gelatin mixture is prepared by using the compound protease comprising the proline protease to perform the enzymatic hydrolysis on the donkey-hide gelatin juice to which no auxiliary material is added. For the active small-molecule donkey-hide gelatin mixture, the weight-average molecular weight ranges from 580 Da to 1300 Da, the peptide segments are distributed from 200 Da to 3000 Da, the dissolution rate in cold water is high, and the content of free amino acid is low; the active small-molecule donkey-hide gelatin mixture may be used for manufacturing small-molecule peptide food and health care food of donkey-hide gelatin.


An active small-molecule donkey-hide gelatin mixture and a preparation method and application thereof. The active small-molecule donkey-hide gelatin mixture is prepared by using the compound protease comprising the proline protease to perform the enzymatic hydrolysis on the donkey-hide gelatin juice to which no auxiliary material is added. For the active small-molecule donkey-hide gelatin mixture, the weight-average molecular weight ranges from 580 Da to 1300 Da, the peptide segments are distributed from 200 Da to 3000 Da, the dissolution rate in cold water is high, and the content of free amino acid is low; the active small-molecule donkey-hide gelatin mixture may be used for manufacturing small-molecule peptide food and health care food of donkey-hide gelatin.


Trademark
Shandong Dong E E Jiao Co. | Date: 2012-08-21

Ginseng for medicinal use, processed bee pollen for medicinal use and propolis for medicinal purposes; medicines for human purposes, pharmaceutical preparations, capsules for medicines and syrups for pharmaceutical purposes, namely, tablets, injections, capsules and syrups, all for use in the treatment of iron-deficiency anemia, hemorrhagic anemia, osteoporosis, hemoptysis, hematochezia, hematuria, menoxenia, postpartum hemorrhage, deficiency of both vital energy and the blood, thrombus, dizziness and blurred vision, palpitation and insomnia, anorexia, leukocyte-poor, thrombocytopenia, asthenia of the kidney, impotence, premature ejaculation, soreness and painfulness of the lumbar region, neurasthenia, common cold, cough, asthma, cardiovascular and cerebro vascular system, and rheumarthritis; medicinal preparations for the mouth to be applied in the form of drops, capsules, tablets and compressed tablets; medicated jujube; ointments for pharmaceutical purposes for use in activating blood circulation and elimination of blood stasis, to relieve muscular contracture, to remove swelling and pain, to treat traumatic injuries, and to eliminate fatigue before and after exercise; dietary food supplements; dietary and nutritional supplements; liquid nutritional supplement; additives in the nature of dietary supplements for humans; medical adhesive plasters; bandages for dressings; medical dressings. Cocoa powder; cocoa-based beverages; cocoa beverages with milk; coffee-based beverages; tea; tea-based beverages; sweetmeats; fondants; sugar; fruit jellies; fruit jelly candy; honey for foods; golden syrup; honey; propolis being bee glue for human consumption; royal jelly for food purposes; processed cereals; cereal-based snack food; rice-based snack food; soya flour; starch for food.

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