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Chen D.,Peking Union Medical College | Cheng B.,Shandong Chest Hospital | Zhou H.-Y.,Tian Jin Medical College | Li L.-H.,Peking Union Medical College
Molecular Biology Reports | Year: 2012

Sevoflurane postconditioning is a potential clinical measure to protect myocardial. This experiment was designed to investigate the efficacy of sevoflurane postconditioning against ischemia-reperfusion injury. A total of 132 Japanese White Rabbits were enrolled into this study. They were underwent 15-, 30-, or 60-min left anterior descending coronary (LAD) artery occlusion, respectively. At the end of LAD artery occlusion, they randomly received a 5-min inhalation of air (control group), 1% sevoflurane (1% sev group), 2%sevoflurane (2% sev group), 4% sevoflurane (4% sev group) or an IV bolus injection of 5 mg/kg of NIM811 [a specific inhibitor of mitochondrial permeability transition pores (mPTP)]. Infarct size was determined after 2 h of reperfusion (triphenyltetrazolium chloride straining, percentage of risk area). The infarct sizes were significantly (P<0.05) reduced after 15 min ischemia (5.5 ± 3.3%, 5.8 ± 3.6% vs. 20.3 ± 6.9% for 2% sev, 4% sev vs. control, respectively) and 30 min ischemia (23.5 ± 5.0%, 20.7 ± 5.9% vs. 50.9 ± 10.2%, for 2% sev, 4% sev vs. control, respectively; P<0.05). However, it had no effect on infarct size after 60 min ischemia (64.1 ± 5.9%, 62.3 ± 7.6% vs. 72.7 ± 9.2% for 2% sev, 4% sev vs. control, respectively, P[0.05).The efficacy of sevoflurane postconditioning gradually weakened with increasing ischemia duration and disappears after 60 min ischemia in rabbits in vivo. © Springer Science+Business Media B.V. 2012.


PubMed | Shandong Chest Hospital and Dongying Peoples Hospital of Shandong provincial Hospital Group
Type: Journal Article | Journal: Journal of biological regulators and homeostatic agents | Year: 2016

The purpose of this study was to observe the change in plasma D-dimer of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The patients were divided into three groups, i.e., AECOPD group, stable COPD group (COPD kept stable after treatment) and a healthy control group. The content of plasma fibrinogen (FIB) and D-dimer of all research subjects was detected and the difference between groups was analyzed. Moreover, pulmonary functions of patients in the AECOPD group and the stable COPD group, including forced expiratory volume in 1 second (FEV1%) and forced vital capacity rate of 1 second (FEV1/FVC), and blood gas (oxygen partial pressure (PO


Wang G.-Q.,Shandong Chest Hospital | Lin M.-Y.,Shandong Chest Hospital
Biological Trace Element Research | Year: 2011

In this study, copper, zinc, magnesium, manganese, selenium, and iron were measured by inductively coupled plasma atomic emission spectrophotometry in patients with tuberculous (TB) pleurisy (n=24) and in the control group (n=20). Selenium, magnesium, and zinc were found to be significantly lower in TB pleurisy cases than those in the control group (p<0.01, p<0.001, and p<0.001, respectively). There was no significant difference in the value of manganese and iron between TB pleurisy and the control group (p>0.05). Copper levels were significantly increased in the serum of the patients (p<0.0001). These results may provide an additional disease correlate for assessing TB pleurisy risk. © 2010 Springer Science+Business Media, LLC.


Wang Q.,Shandong University | Dong Z.,Shandong University | Liu X.,Shandong University | Song X.,Shandong University | And 5 more authors.
Diabetes | Year: 2013

Programmed cell death-4 (PDCD4), a selective protein translation inhibitor, has shown proinflammatory effect in some inflammatory diseases, but its roles in obesity remain unestablished. This study aims to investigate the effects of PDCD4 on obesity, inflammation, and insulin resistance. Surprisingly, high-fat diet (HFD)-fed PDCD4-deficient (PDCD42/2) mice exhibited an absolutely lean phenotype together with improved insulin sensitivity. Compared with wild-type obese mice, HFD-fed PDCD42/2 mice showed higher energy expenditure, lower epididymal fat weight, and reduced macrophage infiltration inflammatory cytokine secretion in white adipose tissue (WAT). Alleviated hepatic steatosis along with decreased plasma levels of triglyceride and cholesterol was also observed in these mice. Importantly, PDCD4 appeared to disturb lipid metabolism via inhibiting the expression of liver X receptor (LXR)-a, a master modulator of lipid homeostasis, which was elevated in HFD-fed PDCD42/2 mice accompanied by upregulation of its target genes and relieved endoplasmic reticulum stress in WAT. These data demonstrate that PDCD4 deficiency protects mice against diet-induced obesity, WAT inflammation, and insulin resistance through restoring the expression of LXR-a, thereby proposing PDCD4 as a potential target for treating obesity-associated diseases. © 2013 by the American Diabetes Association.


PubMed | Shandong Chest Hospital and Shandong University
Type: Journal Article | Journal: Clinical radiology | Year: 2016

To investigate the applicability of diffusion-weighted magnetic resonance neurography (DW-MRN) in the diagnosis of carpal tunnel syndrome (CTS).In total, 47 patients with CTS (69 wrists) and 19 normal participants (38 wrists) was included in this study. Cross-sectional area (CSA) and apparent diffusion coefficient (ADC) values of the median nerves in the carpal tunnel were determined using DW-MRN. Receiver operating characteristic (ROC) analysis was performed.No significant differences in age or body mass index (BMI) were observed between the control and CTS groups. DW-MRN imaging showed obvious hyperintensity in the lesions in CTS wrists, while other nerve regions were related to slight hyperintensity. Interobserver variability analysis indicated excellent agreement regarding both the CSA and ADC measurements for the control and CTS groups. Both the mean CSA and ADC values of the median nerves in carpal tunnel in the CTS group were significantly higher than the control group. According tothe ROC analysis, the CSA cut-off value was 11.7mmDW-MRN represents a highly reproducible diagnostic technique for CTS. The ADC value of median nerves in the carpal tunnel is significantly higher in CTS patients, which provides a potential powerful tool for the disease diagnosis.


PubMed | Yantai City Hospital for Infectious Disease, Shandong Chest Hospital and Shandong University
Type: | Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases | Year: 2016

To measure the expression levels of sputum and serum microRNA-144 (miR-144) before and after the treatment of patients with tuberculosis (TB).Details of the cases of a total of 124 TB patients were collected at Qilu Hospital of Shandong University between April 2014 and April 2015. Fifty-three of these patients had sputum positive for bacteria and a cavity on imaging (group A), 20 patients had sputum negative for bacteria and a cavity on imaging (group B), and 51 patients had sputum negative for bacteria and no cavity on imaging (group C). One hundred seventeen healthy people who attended the hospital for a physical examination were recruited as controls. Quantitative real-time PCR (qRT-PCR) was used to measure the levels of sputum and serum miR-144 before anti-TB treatment and at 1 month after treatment.Before treatment, sputum and serum miR-144 expression levels in the TB patients were both higher than those of the controls (both p<0.05). After treatment, sputum and serum miR-144 levels in the TB patients were significantly lower than those measured before treatment (both p<0.05). The levels of sputum and serum miR-144 in the improved TB patients decreased significantly after treatment compared to those measured before treatment (both p<0.001). Significant differences were found in sputum and serum miR-144 levels in the TB patients, with or without improvement, compared with the healthy controls (all p<0.05).Sputum and serum miR-144 levels were significantly upregulated in the TB patients, but were found to decrease significantly after anti-TB treatment.


PubMed | Shandong Chest Hospital and Shandong University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016

Acute cerebral hemorrhage accounts for approximately 25% of strokes for elderly patients. Consequently, treatments to improve prognosis should be identified. The aim of the present study was to examine the clinical values of the application of clinical nursing pathway for patients with acute cerebral hemorrhage. Between January 2013 and January 2015, 92 patients diagnosed with acute intracerebral hemorrhage were enrolled in the study based on the guidelines recommended for providing appropriate surgical or conservative treatment and the sequence of admission. The 92 patients were randomly divided into the control and observation groups. Patients in the control group underwent routine nursing mode prior to and after admission, and underwent clinical nursing path model (hierarchical partitioning prior to admission to hospital plus general professional program of nursing in hospital) was applied to the observation group. Barthel index scores for the observation group were significantly higher than that of the control group. The length of hospital stay for patients in the observation group was significantly lower while the average score for patients satisfaction on nursing care while in hospital was significantly higher than that of the control group, with statistically significant differences (P<0.05). The incidence of complications such as fever, infection, bedsore, gastrointestinal function, electrolyte disturbances, and malnutrition, in the observation group was significantly lower, with statistically significant differences (P<0.05). The functional independence measure (FIM) and Fugl-Meyer scores after 6 months for the observation group were significantly higher, with statistically significant differences (P<0.05). In conclusion, application of the clinical nursing pathway for patients with acute cerebral hemorrhage significantly improved the clinical effects and nursing satisfaction, reduced adverse reactions, and had a greater clinical application value.


PubMed | Shandong Chest Hospital, Shandong University and Sichuan Academy of Medical science & Sichuan Provincial Peoples Hospital
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016

Whether left atrial (LA) functional abnormalities already exist when the LA is of normal size is unknown. The aim of this study was to explore LA energy loss and mechanics changes using vector flow mapping (VFM) and two-dimensional tissue tracking (2DTT) echocardiography in patients with diabetes and normal LA size.This study included 47 normotensive patients with diabetes and 45 controls. The following indexes were measured: LA energy loss during systole (LAELs), early diastole (LAELed), and atrial contraction (LAELac); atrial longitudinal strain during systole (SLAs), early diastole (SLAed) and late diastole (SLAac); and peak LA strain rate during systole (SRLAs), early diastole (SRLAed), and atrial contraction (SRLAac).The LAELs and LAELed decreased in diabetic patients compared with controls (P=0.002, P<0.01, respectively), whereas the LAELac increased in diabetic patients (P<0.001). The SLAs, SLAed, SRLAs, and SRLAed (all P<0.01) were all lower in diabetic patients than in controls. However, there was no difference in the SLAac and SRLAac between the two groups. Multivariate regression analysis showed that the LAELs, LAELac, and SRLAs were independently associated with HbA(1c) in the whole study population.LA energy loss and deformation mechanics are already impaired in diabetic patients with normal LA size and the long-term parameter of glycemic control was correlated with them. VFM combined with 2DTT might be a promising tool for the early detection of LA dysfunction caused by impaired glucose metabolism.


PubMed | Shandong Chest Hospital, Shandong University and Sichuan Academy of Medical science & Sichuan Provincial Peoples Hospital
Type: | Journal: Polish journal of radiology | Year: 2016

The purpose of this study was to evaluate the reliability, effectiveness, and safety of modified right heart contrast transthoracic echocardiography (cTTE) in comparison with the traditional method.We performed a modified right heart cTTE using saline mixed with a small sample of patients own blood. Samples were agitated with varying intensity. This study protocol involved microscopic analysis and patient evaluation. 1. Microscopic analysis: After two contrast samples had been agitated 10 or 20 times, they underwent a comparison of bubble size, bubble number, and red blood cell morphology. 2. Patient analysis: 40 patients with suspected RLS (right- to-left shunt) were enrolled. All patients underwent right heart contrast echocardiography. Oxygen saturation, transit time and duration, presence of RLS, change in indirect bilirubin and urobilinogen concentrations were compared afterward.Modified method generated more bubbles (P<0.05), but the differences in bubble size were not significant (P>0.05). Twenty-four patients were diagnosed with RLS (60%) using the modified method compared to 16 patients (40%) with the traditional method. The transit time of ASb20 group was the shortest (P<0.05). However, the duration time in this group was much longer (P<0.05). Also, in semi-quantitative analysis mean rank of RLS was higher after injecting the modified contrast agent agitated 20 times (P<0.05).Modified right heart contrast echocardiography is a reliable, effective and safe method of detecting cardiovascular RLS.


PubMed | Shandong Chest Hospital and Shandong Medical Imaging Research Institute
Type: Journal Article | Journal: Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2016

To study the advantages of delayed phase scan at 5 minutes using multi-slice Computed Tomography (CT) for the diagnosis of intracranial tuberculosis.Conventional non-contrast and dual-phase contrast enhancement were performed using 16-slice spiral CT for brain scanning. Arterial phase was performed 25 seconds after the injection of contrast agent, and the delayed phase was performed at 5 minutes. Then the ability of two phase images in intracranial tuberculosis displaying was evaluated.A total of 526 lesions in 30 patients were found. Delayed phase scan was superior to arterial phase in the ability of displaying the thickening of meninges, either basal cistern, pia mater or ependymal (P<0.01). The demonstration of lesion size and margins of tuberculoma was also better in delayed phase scan (P<0.01). The delayed phase scan was significantly better in distinguishing lesions and adjacent vascular sections in the sulcus (P<0.01).The delayed phase scan has obvious advantages in the display of intracranial tuberculosis.

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