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Zhang F.,Shandong Normal University | Huang Y.H.,Food and Fermentation Engineering Key Laboratoiy of Shandong Province | Liu S.Z.,Shandong Normal University | Zhang L.,Shandong Normal University | And 5 more authors.
Environmental Entomology | Year: 2013

Anti-Beauveria bassiana activity of aqueous fecal extracts from conventional German cockroaches [Blattella germanica (L.)] was detected, but was not detected in samples from germ-free German cockroaches. Subsequently, bacterial strain BGI-14 was isolated from the gut of conventional German cockroaches and was identified as Pseudomonas reactans based on 16S rDNA sequence. The strain BGI-14 not only inhibited the germination of conidia, but also inhibited the growth of B. bassiana hyphae. Further studies demonstrated that B. bassiana infections in German cockroaches orally treated with the extracts of BGI-14 fermentation were significantly weakened. Compared with the control group, the cumulative mortality rate of treatment group was reduced by 10.3% at 20 d postinoculation. These studies imply that intestinal flora with anti-B. bassiana activity might contribute to resistance of infection by entomopathogenic fungi. © 2013 Entomological Society of America. Source


Zhang F.,Shandong Normal University | Wang X.J.,Shandong Center for Control and Prevention | Huang Y.H.,Food and Fermentation Engineering Key Laboratory of Shandong Province | Zhao Z.G.,Shandong Center for Control and Prevention | And 5 more authors.
Environmental Entomology | Year: 2014

A proteomic approach combining two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry was used to compare hemolymph expression profiles of a beta-cypermethrin-resistant Blattella germanica L. strain and a beta-cypermethrin-susceptible strain. Twenty-eight hemolymph proteins were differentially expressed in the resistant cockroach strain; 19 proteins were upregulated and 9 proteins were downregulated compared with the susceptible strain. Protein identification indicated that expression of putative cuticular protein, nitric oxide synthase, triosephosphate isomerase, alpha-amylase, ABC transporter, and Per a 3 allergen was elevated, and expression of arginine kinase and glycosidase was reduced. The differential expression of these proteins reflects the overall change in cellular structure and metabolism related to the resistance of pyrethroid insecticides. © 2014 Entomological Society of America. Source


Wei X.,Chinese University of Hong Kong | Zou G.,University of Leeds | Zhang H.,Shandong Center for Control and Prevention | Walley J.,University of Leeds | And 4 more authors.
Tropical Medicine and International Health | Year: 2011

Objective In compliance with Chinese national policy, Shandong province set up microscopy centres (MCs) in one-third of all township hospitals where sputum smear microscopy is performed. We assessed the centres' performance and any factors influencing it. Methods A cross-sectional, structured, facility-based questionnaire survey was conducted in 2006, collecting data from all 113 counties with established MCs as well as tuberculosis (TB) routine reporting data from the county TB dispensary. Results One hundred and thirteen of 142 counties in Shandong had established 497 MCs. The centres identified 3.3% of smear-positive patients in the province. Sixty-six percentage of MCs did not identify any patients with TB; only 5% identified more than 10. Poor performance arose from low quality of staff training in township hospitals, but inappropriate geographical provision of services was the main cause of low additional case finding. Conclusion The MC policy did not achieve its goal of improving TB case finding in Shandong. © 2011 Blackwell Publishing Ltd. Source


Huang Y.H.,Shandong Normal University | Wang X.J.,Shandong Center for Control and Prevention | Zhang F.,Shandong Normal University | Huo X.B.,Shandong Center for Control and Prevention | And 5 more authors.
Journal of Economic Entomology | Year: 2013

A bacterial strain BGI-1 was isolated from the gut of German cockroaches (Blattella germanica L.) and was identified as Bacillus subtilis based on 16S rDNA sequence and morphological, physiological, and biochemical characters. The strain BGI-1 inhibited the growth of Beauveria bassiana; the diameter of the inhibition zone exceeded 30 mm. Vesicles were observed in B. bassiana hyphae on the edge of the inhibition zone. Fermentation of BGI-1 reduced the conidial germination rate by 12%. Further studies demonstrated that B. bassiana infections in German cockroaches orally treated with the extracts of BGI-1 fermentation were significantly weakened. Cumulative mortality rate was 49.5% in the treatment group at the 20 d, while that of the control group was 62.3%. The study intends to understand the relationship between the intestinal flora and the cockroach. Those microbes with anti-entomopathogenic fungi activity might contribute to resisting the infection of pathogenic fungi. © 2013 Entomological Society of America. Source

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