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Liang J.,Shandong Boly lely Bioengineering Cooperation | Cheng F.-L.,Shandong Boly lely Bioengineering Cooperation | Chen T.-T.,Shandong Boly lely Bioengineering Cooperation | Su Z.-R.,Shandong Boly lely Bioengineering Cooperation | Fan Q.-P.,Shandong Boly lely Bioengineering Cooperation
Chinese Journal of Biologicals

Objective To develop a one-step RT-PCR assay for Newcastle disease virus (NDV). Methods A pair of specific primers was designed according to the sequences of F gene of NDV, based on which a one step RT-PCR assay was developed and verified for specificity and sensitivity. Twenty-three clinic suspected samples were detected by the developed RT-PCR method, of which the results were compared with those by hemagglutination (HA) and hemagglutination inhibition (HI) tests. Results By developed RT-PCR method, the detection result of NDV was positive, while those of infectious bursal disease virus (IBDV) and avian influenza virus (AIV) subtype H9 were negative. The minimum detection limit of the method was 4 pg NDV RNA. Of the 23 clinic suspected samples, 11 were judged as positive by RT-PCR, and 13 by HA and HI tests, indicating a positive coincidence rate of 85%. Conclusion One-step RT-PCR assay for NDV was developed, which was specific, sensitive and time-saving, and might be used for early rapid diagnosis and epidemiological investigation of NDV at molecular level. Source

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