Shandong Academy of Sciences

Qingdao, China

Shandong Academy of Sciences

Qingdao, China

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Zhang K.,Shandong Academy of Sciences | Song L.,Shandong Cancer Hospital and Institute
PLoS ONE | Year: 2014

Background: The associations between vitamin D receptor (VDR) gene polymorphisms and breast cancer risk were comprehensively investigated to clarify issues that remain controversial. Methodology/Principal Findings: An electronic search was conducted of several databases, including PubMed, the Cochrane library, Web of Science, EMBASE, CBM and CNKI, for papers that describe the association between Fok1, poly-A repeat, Bsm1, Taq1 or Apa1 polymorphisms of the VDR gene and breast cancer risk. Summary odds ratios and 95% confidence intervals (CI) were estimated based on a fixed-effect model (FEM) or random-effect model (REM), depending on the absence or presence of significant heterogeneity. A total of 39 studies met the inclusion criteria. A meta-analysis of high-quality studies showed that the Fok1 polymorphism of the VDR gene was associated with an increased risk of breast cancer (ff vs. Ff+FF, OR: 1.09, 95%CI: 1.02 to 1.16, p = 0.007). No significant associations were observed between the other polymorphisms and breast cancer risk. No positive results were detected by pooling the results of all relevant studies. Conclusion: A meta-analysis of high-quality studies demonstrated that the Fok1 polymorphism of the VDR gene was closely associated with breast cancer risk. © 2014 Zhang, Song.


An electromagnetic radiation field within a biological organism is characterized by non-local interference. The interfering beams form a unitary tridimensional network with beams of varying intensity, also called striae, which are distributed on the organism surface. These striae are equivalent to semi-reflectors. The striae carry bio-information of corresponding organs and, thus, integrate all tissues, and organs of the organism. The longitudinal striae are classified as channels, while the transverse striae are collaterals. The acupoints are seen as the points where electromagnetic interfering striae intersect or converge. This hypothesis builds a foundation to understand the traditional Chinese medicine, including acupuncture, from the perspective of scientific knowledge. © 2011 Springer Science+Business Media, LLC.


Duan C.-X.,Shandong Academy of Sciences | Liu Y.-G.,Shandong Academy of Sciences
Current Medicinal Chemistry | Year: 2013

Hydrogen sulfide (H2S), known for its unpleasant rotten egg smell and its high toxicity, has recently emerged as an important mediator of human physiological and pathological processes, such as the regulation of cell growth, cardiovascular protection, the stimulation of angiogenesis, gastric mucosal injury and Alzheimer's disease. Due to its significant actions in the physiology, H2S has attracted the abundant concern of numerous researchers in the cutting edge of chemistry, biology and medicine. Recently, several fluorescent probes have been developed for detecting and elucidating the role played by H2S in biological systems. This review highlights recent advances that have been made on the mechanism and applications of fluorescent probes for the detection of H2S, demonstrating a new field in which remarkable improvements have been accomplished over the last two years.


Li Z.J.,Shandong Academy of Sciences
Zhonghua zhong liu za zhi [Chinese journal of oncology] | Year: 2013

To investigate the effect of high mobility group box-1 (high mobility group box B 1, HMGB1) on the invasive and metastatic abilities of gastric cancer cell line MGC-803 and analyze the possible mechanisms. HMGB1 gene targeting siRNA was designed and synthesized, and HMGB1 siRNA oligonucleotides were transfected into the MGC-803 cells with Lipofectamine 2000. The invasive and migratory abilities were detected by transwell assay and scratch assay. The Matrigel matrix glue adhesive ability of MGC-803 cells was evaluated by MTT assay. NF-κB activity was detected by electrophoretic mobility shift assay. The mRNA and protein levels of HMGB1 and MMP-9 were determined by RT-PCR and Western blot, respectively. The siRNA down-regulated the levels of HMGB1 mRNA and protein. Compared with that of the control group, the number of invasive (142.7 ± 3.4 /view vs. 303.5 ± 4.3/view) and migratory (293.7 ± 4.4/view vs. 445.5 ± 5.6/view) cells was significantly increased (P < 0.05) and the adhesive ability of MGC-803 cells to Matrigel was significantly elevated (33.4 ± 0.03% vs. 57.4 ± 4.2%, P < 0.05). In addition, silencing of HMGB1 gene significantly inhibited the activity of NF-κB and the relative expression folds of mRNA (0.2 ± 0.1 vs. 1.4 ± 0.4, P < 0.05)and protein (0.4 ± 0.1 vs. 2.3 ± 0.7, P < 0.05) of MMP-9. Silencing of HMGB1 can effectively inhibit the invasion and migration of gastric cancer cells and this effect of HMGB1 may be partly due to its regulation of NF-κB and MMP-9 expressions.


Yang Y.,Shandong Academy of Sciences
Materials Science and Engineering B: Solid-State Materials for Advanced Technology | Year: 2013

LaPO4:Eu3+ microspheres were synthesized, using LaCl3, EuCl3 and (NH4)2HPO 4 as starting materials. The morphology, formation mechanism, and luminescent property of samples were systemically studied. X-ray diffraction (XRD) and infrared spectroscopy (IR) show that LaPO4:Eu3+ microspheres have a pure monoclinic phase. Cetyltrimethyl ammonium bromide (CTAB) usually forms spherical micelles above a critical micelle concentration, which plays an important role in the formation of LaPO4:Eu 3+ microspheres. The excitation spectrum of LaPO4:Eu 3+ microspheres consists of several sharp lines due to the direct excitation of the Eu3+ cations from the ground state to higher levels of the 4f-manifold. The emission intensity of microspheres is higher than irregular particles because of the lowlier surface area. The lifetimes of Eu3+ ions in the LaPO4:Eu3+ microspheres are determined to be 2.41 ms. © 2013 Elsevier B.V. All rights reserved.


Determine cyclin-dependent kinase inhibitor 2A (p16(Ink4a)) and polycomb ring finger oncogene (Bmi1) expression in corneal endothelium samples from different age groups and test whether the expression of p16(INK4a) and Bmi1 are associated with endothelial cellular senescence in human cornea. Samples were selected from an eyebank of healthy human corneal endothelial cells (HCECs). Donor human corneas were divided into three age-groups: age ≤30 years, 30-50 years and ≥50 years. The expression of p16(INK4a) and Bmil were analyzed by real-time PCR, immunohistochemistry, and immunofluorescence. Through real-time PCR, we detected less than threefold decreases in Bmi1 expression and greater than fivefold increases in p16(INK4a) expression associated with aging. Bmi1 expression was significantly down-regulated with increasing donor age. The number of p16(INK4a)-positive cells was significantly higher and the number of Bmi1-positive cells was significantly lower in older donors compared to the younger age groups. Our immunohistochemistry experiments showed that the expression of p16(INK4a) in older donors was stronger than that in younger donors and the expression of Bmi1 in older donors was weaker than that in younger donors. Results from both the immunohistochemistry and real-time PCR experiments confirmed increased expression of p16(INK4a) and decreased expression of Bmi1 with age in HCECs. Additionally, the results of immunofluorescence double-staining for p16(INK4a) and Bmi1 further validated the immunocytochemistry and real-time PCR results. Our data are the first to demonstrate that high expression of p16(INK4a) and low expression of Bmi1 are associated with endothelial cellular senescence in human cornea. Our findings are not just for cornea transplantation but also for a better understanding of the cornea senescence and the process of aging in this specific human organ.


Chen M.,Shandong Academy of Sciences | Xie L.,Shandong Academy of Sciences
Ophthalmology | Year: 2013

Purpose: To study long-term clinical patterns of recurrence of anterior corneal pathologies after excimer laser phototherapeutic keratectomy (PTK). Design: Retrospective, noncomparative, interventional case series. Participants: Thirty patients (44 eyes) with anterior corneal pathologies who underwent PTK and experienced recurrence after long-term follow-up between March 1997 and April 2012. Preoperative diagnoses included band keratopathy in 7 eyes, anterior basement membrane dystrophy (ABMD) in 8 eyes, granular dystrophy in 15 eyes, lattice dystrophy in 10 eyes, and macular corneal dystrophy (MCD) in 4 eyes. Methods: Data of each patient were collected regarding the recurrence of primary disease after PTK. RTVue (Optovue, Inc., Fremont, CA) optical coherence tomography (OCT) was used to detect the depth of recurrent deposits. Confocal microscopy was used to evaluate the cellular alterations associated with recurrent corneal disease. Main Outcome Measures: Interval, location, morphology of the recurrence, depth of recurrent deposits, and cellular alterations in recurrent disease. Results: The mean follow-up (from the first PTK to the last visit) was 95 months (range, 80-120 months). The disease recurrence was symptomatic in all 8 eyes treated for ABMD and 2 eyes treated for lattice dystrophy and asymptomatic in the other cases. Significant recurrence of band keratopathy, ABMD, MCD, lattice dystrophy, and granular dystrophy developed at an average of 7.8, 12.4, 13.5, 19.7, and 23.7 months after PTK, respectively. RTVue OCT images indicated that the recurrent deposits involved the anterior corneal stroma (80-150 μm) and were mainly within 8 mm around the corneal center. Morphologic changes included disorganized stromal fibers, decreased density and disordered arrangement of nerve fibers, and inconspicuous keratocyte nuclei, occasionally accompanied by decreased keratocyte density and endothelial cell density. Conclusions: The features of disease recurrence after PTK are closely related to the original corneal pathology. Recurrence in this series was fastest in patients with band keratopathy and, sequentially, ABMD, MCD, lattice dystrophy, and granular dystrophy. RTVue OCT imaging and confocal microscopy were valuable tools for the diagnosis of recurrent corneal opacities. Financial Disclosure(s): The author(s) have no proprietary or commercial interest in any materials discussed in this article. © 2013 American Academy of Ophthalmology.


Patent
Shandong Academy of Sciences | Date: 2013-08-15

The invention relates to a preparation method of vinyl-terminated siloxane. R^(2)R^(3)Si(OR^(4))_(2), R^(1)_(2)(CH_(2)CH)SiOSi(CHCH_(2))R^(1)_(2), an organic acid and a catalyst are added into a reaction bottle according to the molar ratio of 1:1-1.5:1-2.5:0.005-0.01, wherein R^(1 )is alkyl, R^(2 )and R^(3 )are alkyl, aryl or substituted alkyl, substituted aryl identically and differently, and R^(4 )is alkyl, heating is performed to 50-80 C. while stirring, the stirring reaction is performed at the temperature for 5-30 min, then a dehydrating agent is added, and the reaction is continuously performed for 2-5 h at the temperature of 50-80 C.; and the temperature is reduced to room temperature after the end of reaction, and then dilution, washing with water to neutral, collection of an organic phase, drying, filtering, concentration, reduced pressure distillation and collection of a fraction at corresponding pressure and temperature are sequentially performed so as to obtain a vinyl-terminated siloxane compound product.


The bacterium Arthrobacter ureafaciens liulou 1 (CGMCC 9667) possesses a unique combination of high atrazine-degrading activity, a capability of colonizing plant roots after seed inoculation and traits of a plant growth promoting bacterium. Also disclosed is a method of A. ureafaciens liulou 1 (CGMCC 9667) application for remediation of the polluted soils and plant protection from atrazine.


Wang Y.,Shandong Academy of Sciences
Investigative ophthalmology & visual science | Year: 2013

To investigate how Sirtuin (silent mating type information regulation 2 homolog) 1 (SIRT1) promotes high glucose-attenuated corneal epithelial wound healing. The effects of high glucose on SIRT1 expression were assessed in primary human corneal epithelial cells (CECs) in treatment of 5 mM d-glucose (normal glucose [NG]) and 25 mM D-glucose (high glucose [HG]) and corneas from Ins2(Akita/+) mice by Western blotting. The osmotic pressure of the NG medium was adjusted to that of the HG medium by adding 20 mM mannitol. Pifithrin-α (PFT-α) was used to inhibit the expression of p53 and an adenovirus was used for overexpression of SIRT1 in vivo and in vitro. How overexpression of SIRT1 promotes HG-attenuated corneal epithelial wound healing via p53 regulation of the IGFBP3 (insulin-like growth factor binding protein-3)/IGF-1 (insulin-like growth factor-1)/AKT pathway was investigated in CECs and Ins2(Akita/+) mice. HG induced the downregulation of SIRT1 and the upregulation of p53 acetylation in primary human CECs and corneas from Ins2(Akita/+) mice. The results of cell migration assay and corneal wound healing from Ins2(Akita/+) mice demonstrated that SIRT1 overexpression strongly promoted wound healing in the presence of HG levels via the downregulation of the IGFBP3 protein. The levels of total p53 expression and acetylated p53 decreased dramatically in the presence of PFT-α, whereas the IGF-1R/AKT pathway was activated in CECs. The results of cell migration assay suggested this posttranslational modification of p53 was involved in the response to cell injury under HG conditions in CECs. The molecular mechanism by which SIRT1 promotes corneal epithelial wound healing was involved in an enhancement of the IGFBP3/IGF-1/AKT pathway through the deacetylation of p53. This study also suggests that SIRT1 has a protective role in the pathogenesis of diabetic keratopathy.

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