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PubMed | Email and Shandong Academy of Occupational Health and Occupational Medicine
Type: Journal Article | Journal: Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] | Year: 2016

To analyze the expression levels of heat shock protein70 (HSPs70) and HSPs70 mRNA in different exposure to manganese, and research the neuroprotective effect on the career exposure to manganese.From 2008 to 2009, with cross-sectional study design, and in a locomotive and rolling stock works, by stratified random sampling method, the exposed sample consisted of 180 welders from different welding shops and 100 unexposed in the last three years, non-welder controls with age-matched workers of similar socioeconomic status from the same industry. The control workers had not been exposed to neurotoxic chemicals. The mRNA expressions of four different metabolic enzyme were detected by SYBR Green I quantitative real-time polymerase chain reaction. The expression levels of the two enzymes mRNA in different exposure to manganese were analyzed. The expressions of HSPs70 were detected by Western blot. The concentration of air manganese was determined by GFAAS. The average concentration of 8 h time (8h-TWA) was used to express the level of individual exposure to manganese, according to the air manganese workplace occupational exposure limit (8h-TWA=0.15 mg/m3), the exposed group is divided into high exposed group (>0.15 mg/m3) and low exposure group (<0.15 mg/m3).The individuals exposed to manganese dose of exposed group ((0.250.31) mg/m3) was higher than the control group ((0.060.02) mg/m3) (t=6.15, P=0.001); individuals exposed to manganese dose of high exposure group for (0.420.34) mg/m3, which was higher than low exposure group (0.090.07) mg/m3 (t=9.80, P=0.001). HSPs70 mRNA and protein of exposure group (5.650.21, 3.260.15) were higher than the reference group (0.410.03, 1.320.12) (t=18.91, t=8.68, P=0.001). HSP70 mRNA and protein of high exposure group (6.480.37, 3.670.26) were higher than the low exposure group (5.150.23, 3.020.19) (t=3.24, t=2.01, P=0.003, P=0.043).The expression of peripheral blood lymphocytes HSPs70 level and HSPs70 mRNA workers exposed to manganese increased and protect nerve cells from related to Mn stimulation induced lipid peroxidation damag.


Wu H.,Chinese National Institute for Communicable Disease Control and Prevention | Wang H.,Shandong University | Wang Q.,Shandong University | Xin Q.,Shandong Academy of Occupational Health and Occupational Medicine | Lin H.,Guangdong Provincial Institute of Public Health
Global health action | Year: 2014

Background : Hand, foot, and mouth disease (HFMD) is a contagious viral illness that commonly affects infants and children. This infection is an emerging infectious disease in Rizhao in recent years. The present study examined the short-term effects of meteorological factors on adolescent HFMD in Rizhao. Design : A generalized additive Poisson model was applied to estimate the effects of meteorological factors on adolescent HFMD occurrence in 2010-2012. Subgroup analyses were also conducted to examine the potential effect modifiers of the association in terms of age, sex, and occupation. Results : A positive effect of temperature was observed (ER [excess risk]=1.93%, 95% CI: 1.05 to 2.82% for 1°C increase on lag 5 day). A negative effect of relative humidity at lag 1 day and positive effects were found on lag 5-7 days, and an adverse effect was observed for sunshine at lag days 3-4 (ER=-0.71%, 95% CI: -1.25 to -0.17% on lag day 4). We also found that age, sex, and occupation might be important effect modifiers of the effects of weather variables on HFMD. Conclusions : This study suggests that meteorological factors might be an important predictor of adolescent HFMD occurrence in Rizhao. Age, sex, and occupation might be important effect modifiers of the effects.


Song L.Q.,Shandong Academy of Occupational Health and Occupational Medicine
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2012

To establish the method of detecting the concentrations of methyl isocyanate (MIC) in air of workplaces with high performance liquid chromatographic (HPLC). Samples are collected by XAD-7 tubes coated with 1-(2-pyridyl) piperazine (1-2PP). Samples are desorbed with acetonitrile (ACN) and analyzed by high performance liquid chromatography (HPLC) using a fluorescence detector. There was a linear relationship within the range of 0.01 ∼ 10 μg/ml, and the detection limit was 5.3×10(-4) μg/ml. The minimum detectable concentration was 0.01 μg/ml, and the lowest detected concentration was 3.3×10(-3) mg/m(3). The relative standard deviation was 2.2% ∼ 5.3%. The average desorption efficiency was 90% and the sampling efficiencies were 100%. The samples could be stored for 10 days in cold storage condition. The present method could meet with the requirements of Guide for establishing occupational health standards-Part4 Determination methods of air chemicals in workplace and be feasible for determination of MIC in workplace air.


Chen W.W.,Shandong Academy of Occupational Health and Occupational Medicine
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2010

To investigate the relationship between genetic polymorphism of dopamine β-hydroxylase (DBH) and manganese-induced nerve injury. In a cross-sectional study, 402 electric welders who had worked over one year in relatively fixed sites were recruited, and the concentration of manganese in which they worked was stable. These samples was divided into high exposure group (CEI > 1) and low exposure group (CEI < 1) by CEI. Between the two groups, the groups were divided into abnormal group and normal group according to the result of neurologic check (there were 81 workers with abnormal neurological dysfunction in high exposure group and 28 workers in low exposure group, P < 0.05). Polymorphism of DBH gene was analyzed with polymerase chain reaction-restriction fragment length polymorphism. The distribution of A2A2 genotype and A2 allele of DBH was significantly different. In high exposure group, the distribution of A2A2 genotype and A2 allele of DBH in abnormal group was significantly wider than in normal group (A2A2 genotype, OR = 1.248, P < 0.05, A2 allele, OR = 1.103, P < 0.05). In low exposure group, the distribution of A2 allele of DBH in abnormal group was significantly wider than in normal group (OR = 1.176, P < 0.05). The individuals who carry A2A2 genotype and A2 allele of DBH have increased risk of neurological dysfunction after explosion to manganese for a certain time, which suggests that polymorphism of DBH (intron 5 Taq I) would play a great role in hereditary susceptibility of neurological dysfunction cause by manganese.


Zhang Z.,Shandong Academy of Occupational Health and Occupational Medicine
Wei sheng yan jiu = Journal of hygiene research | Year: 2010

To study the effects of zeranol on spermiogenesis function of mice and its possible mechanism. 40 healthy mature male mice were randomly divided into four groups: negative group and zeranol exposure groups (25, 50, 100 mg/kg). Male mice were respectively administered with zeranol at the doses of 25, 50, and 100 mg/kg for 35 days and killed. The weights of spermary, epididymis and spermatocyst were measured. The sperm counting, motility and the percent of abnormal sperms were observed. Pathological changes of testicle tissue were also observed. In comparison with control group, the visceral coefficient of spermatocysts and sperm counting of zeranol exposure groups were all decreased ( P < 0.05). Sperm motility were significantly decreased in the median and high exposure groups (P < 0.05). Pathological alterations of testes were also observed. Seminiferous epithelium was reduced in exposure groups, loose and anomalistic organization. Sperm counts were also reduced in the lumina of seminiferous tubules with glair. Zeranol have toxicity effect on spermigenesis function of male mice.


Li Y.,Shandong Academy of Occupational Health and Occupational Medicine
Wei sheng yan jiu = Journal of hygiene research | Year: 2010

To explore the relation between genetic polymorphisms in XPD and risks of chronic benzene poisoning (CBP). A case-control study was conducted. 152 CBP patients and 152 NCBP workers occupationally exposed to benzene were investigated. Polymerase chain reaction-restrained fragment length polymorphism technique (PCR-RFLP) was applied to detect the single nucleotide polymorphisms (SNPs) at c. 199, c. 201, c. 312 and c. 751 of XPD gene. No variant alleles was detected at c. 199 and c. 201 of XPD gene. In comparition with the individual genotypes of XPDc. 312Asp/Asp, the risk of CBP suffered from the individual genotype of XPDc. 312Asp/Asn + Asn/Asn decreased a 0.59 fold (ORadj = 0.59, 95% CI = 0.35-0.99, chi2 = 3.99, P < 0.05), when sex, workage and intensity of benzene exposure were adjusted. And in low intensity of benzene exposure group, the risk of CBP suffered from the individual genotypes of XPDc. 312Asp/Asn + Asn/Asn more decreased (ORadj = 0.13, 95% CI = 0.04-0.51, chi2 = 8.93, P < 0.01). Polymorphism of XPD Asp312Asn could contribute to altered risk of CBP.


Cai S.L.,Shandong Academy of Occupational Health and Occupational Medicine
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011

To study the relationship between polymorphisms of MnSOD and the susceptibility of chronic poisoning exposed to manganism occupationally. In a study of case-control, genotypes were determined by PCR-RFLP in 164 patients with chronic occupational mangamism poisoning and 328 controls with age- and sex-matched for MnSOD 9Ala-Val. There was a significant difference in the frequency of MnSOD 9Ala-Val at V locus mutant allele between cases and controls (χ(2) = 15.225, P < 0.01, 95%CI = 1.43 ∼ 3.00). Individuals with the genotype VV had a 1.30 of risk increase of occupational chronic manganism poisoning compared with the the genotype AV or AA (OR = 2.30, 95%CI = 1.52 ∼ 3.49, P < 0.05). The MnSOD polymorphisms may be related with the susceptibility to chronic occupational manganism poisoning, the risk of chronic occupational manganism poisoning increases in carriers with genotype VV at MnSOD 9Ala-Val locus.


Pan X.,Shandong Academy of Occupational Health and Occupational Medicine
Wei sheng yan jiu = Journal of hygiene research | Year: 2010

To evaluate the protective effect of gulonic acid lactone in the gas chromatography-mass spectrometry system for detecting phorate, diazinon, dimethoate, methyl parathion, malathion five kinds of organic phosphorus pesticide. By comparing the peak area changes of organic phosphorus pesticide configured with different concentrations (0, 0.3, 0.6, 0.9 and 1.2 mg/ml) of the gulonic acid lactone-methanol solution, and the concentrations (500 ng/ml) of organic phosphorus pesticide are same, to determine the best dosage of gulonic acid lactone. In the concentration, evaluate the protective effect of gulonic acid lactone on five kinds of organic phosphorus pesticide testing. When the concentration of gulonic acid lactone-methanol solution <1 mg/ml, the selected ion peak area of the five kinds organophosphorus pesticides detected by gas chromatography-mass spectrometry in the same conditions increases with the upward trend of its concentration ; when the concentration is equal to 1 mg/ml, the peak area reached the highest level, and then with the concentrations increased, no significant change in it. Diazinon's sensitivity increased by 4 times, phorate's sensitivity increased by 5 times, dimethoate, methyl parathion and malathion's sensitivity increased by 10 times, and the peak shape improved distinctly, tailing phenomenon disappears. Gulonic acid lactone can effectively improve the five kinds of organic phosphorus pesticide's sensitivity, improving the peak-type tailing and asymmetric phenomenon by the result of the active site. Quantitative bias can be effectively corrected caused by matrix effects. When the concentration is 1 mg/ml, it has the best effect of the compensation effect.


Zhang F.,Shandong Academy of Occupational Health and Occupational Medicine
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2013

To investigate the role of genetic polymorphisms of epoxide hydrolase 1 (EPHX1) in the metabolism of styrene in vivo. Fifty-six styrene-exposed workers, who worked in the painting workshop of an enterprise for manufacturing glass fiber-reinforced plastic yachts in Shandong Province, China for over one year and were protected in approximately the same way, were selected as study subjects. The 8-hour time-weighted average concentration (8 h-TWA) of styrene and the concentrations of mandelic acid (MA) and phenyl glyoxylic acid (PGA) as urinary metabolites were measured. The genetic polymorphisms of EPHX1 were detected by polymerase chain reaction-restriction fragment length polymorphism analysis. The urinary concentrations of MA and PGA were 177.25±82.36 mg/g Cr and 145.91±69.73 mg/g Cr, respectively, and the 8 h-TWA of styrene was 133.28±95.81 mg/m3. Urinary concentrations of MA and PGA were positively correlated with 8 h-TWA of styrene (R=0.861, P < 0.05; R=0.868, P < 0.05). The subjects were divided into high-exposure group (8 h-TWA >50 mg/m(3)) and low-exposure group (8 h-TWA ≤ 50 mg/m(3), and in the two groups, the urinary concentrations of MA and PGA were significantly higher in the individuals carrying high-activity genotypes of EPHX1 than in those carrying low-activity genotypes of EPHX1 (P < 0.05). Genetic polymorphisms of EPHX1 play an important role in the metabolic process of styrene in vivo.


Zhang W.,Shandong Academy of Occupational Health and Occupational Medicine
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2013

To investigate the changes in the levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) in rats exposed to silica dust. Experimental rats were randomly divided into control group and three experimental groups (doses of dust: 15, 30, and 60 mg/ml), with 42 rats in each group. Each rat in the control group was treated with 1 ml of normal saline by intratracheal instillation, while each rat in the experimental groups was exposed to 1 ml of silica suspension by a single intratracheal instillation. Seven rats in each group were killed at 1, 3, 7, 14, 21, and 28 days after exposure, and then BALF was collected. Enzyme-linked immunosorbent assay was used to measure the levels of interleukin (IL)-1, IL-6, IL-16, macrophage inflammatory protein-1 alpha (MIP-1α), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β). The levels of cytokines in each experimental group were higher than those in the control group at any time point. In the early stage of exposure (day 1-3), BALF IL-1 level increased significantly with the increase in dust dose, and on day 14, BALF IL-6 and IL-16 levels increased significantly with the increase in dust dose; the levels of IL-1, IL-6, and IL-16 in the experimental groups reached the peak on day 14. There were significant differences in the levels of MIP-1α and MCP-1 between the experimental groups (FMIP-1α = 30.106, P<0.01; FMCP-1 = 17.193, P<0.01). In each group, the level of MCP-1 varied significantly at different time points (F = 0.618, P>0.05). On day 1-14, BALF TNF-α level increased with the increase in dust dose, with a significant dose-response relationship (P < 0.05). In each experimental group, TNF-α level reached the peak on day 14. On days 14, 21, and 28, the high-dose group had significantly higher BALF TGF-β levels than the low-dose group (P<0.05); on days 14 and 28, the high-dose group had significantly higher BALF TGF-β levels than the middle-dose group (P<0.05). IL-1, IL-6, IL-16, MIP-1α, MCP-1, and TNF-α play a role in the development and progression of silicosis inflammation. TGF-β may be related to (related to; associated with; correlated with) fibrosis.

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