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Zhao Y.,Beijing University of Chemical Technology | Tang G.-S.,Beijing University of Chemical Technology | Yu Z.-Z.,Beijing University of Chemical Technology | Qi J.-S.,Shandong Academy of Agricultural Sciences
Carbon | Year: 2012

Graphite oxide (GO)/ordered polyaniline (PANI) composites have been prepared through an in situ polymerization. TEM, XRD, FTIR and XPS analyses show that the PANI grew along the surface of exfoliated GO as a template to form a more ordered structure with high crystallinity during polymerization. Compared with pure PANI, both higher electrical conductivity and higher Seebeck coefficient of GO/PANI composites result from the increased carrier mobility, which is confirmed by Hall measurement. Strong interactions exist between graphene oxide and PANI, including electrostatic forces, hydrogen bonding and π-π stacking. There is no significant difference in thermal conductivity between GO/PANI composites and PANI. The maximum electrical conductivity and Seebeck coefficient of the composites reach 751 S m -1 and 28.31 μV K -1, respectively. The maximum thermoelectric figure of merit is up to 4.86 × 10 -4, 2 orders of magnitude higher than that of pure PANI. © 2012 Elsevier Ltd. All rights reserved.

Shandong Qihe Bio Technology Co. and Shandong Academy of Agricultural Sciences | Date: 2013-04-07

A method for increasing the yield of total flavonoids in Ganoderma lucidum mycelium by an expansin comprises: (1) inoculating Ganoderma lucidum into the PD liquid fermentation medium, activating the culturing of the strains and obtaining a seed solution; (2) inoculating the seed solution into a liquid fermentation medium, culturing and adding the expansin solution, then further culturing, isolating and obtaining Ganoderma lucidum mycelium; (3) extracting flavonoids from the Ganoderma lucidum mycelium and obtaining total flavonoids. A plant expansin is used for the liquid fermentation production of flavonoids ingredients of Ganoderma lucidum, and the fermentation process and extraction method are optimized to increase greatly the yield of total flavonoids in Ganoderma lucidum.

Kong L.,Shandong Academy of Agricultural Sciences
Agronomy for Sustainable Development | Year: 2014

Crop residue retention is a key component of sustainable cropping systems. In recent years, retention of crop residue is a means of improving soil quality and nutrient capacity and reducing the adverse effects of residue burning. Maize-wheat rotation is a major double-cropping system practiced on more than one fifth of agricultural lands worldwide. Currently, more than 50 % of maize residues are retained immediately after harvesting. In China, this practice has beneficial effects on soil properties and productivity of the succeeding wheat crop. However, increasing the retention of maize residues without proper soil management has also led to a series of concerns, such as short-term nitrogen immobilization, rapid moisture loss, and high susceptibility to freezing during winter as a result of increased soil porosity. Therefore, other practices for maize residue retention are needed to promote wheat growth and the release of nutrients. This article reviews recent developments in China concerning the consequences of maize residue retention on the physical, chemical, and biological properties of soil quality and the growth, productivity, and quality of wheat. Major patterns of maize residue retention are discussed. In particular, this review provides three complementary approaches for maize residue management. © 2013 INRA and Springer-Verlag France.

BACKGROUND: In wheat (Triticum aestivum L), the flag leaf has been thought of as the main source of assimilates for grain growth, whereas the peduncle has commonly been thought of as a transporting organ. The photosynthetic characteristics of the exposed peduncle have therefore been neglected. In this study, we investigated the anatomical traits of the exposed peduncle during wheat grain ontogenesis, and we compared the exposed peduncle to the flag leaf with respect to chloroplast ultrastructure, photosystem II (PSII) quantum yield, and phosphoenolpyruvate carboxylase (PEPCase; EC activity. RESULTS: Transmission electron microscope observations showed well-developed chloroplasts with numerous granum stacks at grain-filling stages 1, 2 and 3 in both the flag leaf and the exposed peduncle. In the exposed peduncle, the membranes constituting the thylakoids were very distinct and plentiful, but in the flag leaf, there was a sharp breakdown at stage 4 and complete disintegration of the thylakoid membranes at stage 5. PSII quantum yield assays revealed that the photosynthetic efficiency remained constant at stages 1, 2 and 3 and then declined in both organs. However, the decline occurred more dramatically in the flag leaf than in the exposed peduncle. An enzyme assay showed that at stages 1 and 2 the PEPCase activity was lower in the exposed peduncle than in the flag leaf; but at stages 3, 4 and 5 the value was higher in the exposed peduncle, with a particularly significant difference observed at stage 5. Subjecting the exposed part of the peduncle to darkness following anthesis reduced the rate of grain growth. CONCLUSION: Our results suggest that the exposed peduncle is a photosynthetically active organ that produces photosynthates and thereby makes a crucial contribution to grain growth, particularly during the late stages of grain-filling.

Dong H.,Shandong Academy of Agricultural Sciences
Australian Journal of Crop Science | Year: 2012

Soil salinity is a major threat to cotton production worldwide. Excessive salt in the soil leads to a series of physiological and biochemical metabolic disorders in cotton plants mainly as a result of osmotic effects (dehydration), nutritional imbalance and toxicity of salt ions (Na+ and Cl-). The metabolic disorders may finally reduce plant growth and lint yield and quality, particularly in conditions of high salinity. Basically, combating the effects of salinity stress on cotton plants involves two main strategies: one is to improve salt tolerance through genetic breeding and chemical or biological treatment, the other is to avoid or alleviate salinity stress by improving at least part of the root-zone environment. This review highlights the technologies for combating salinity stress on cotton, with a focus on recent advances in agronomic techniques for managing salinity in the root zone. We recommend a comprehensive use of agronomic practices such as suitable cultivars, proper irrigation and fertilization, seed pretreatment, furrow seeding, plastic mulching and induction of unequal salt distribution in the root-zone to combat salinity stress. Further research should focus on exploration and understanding of the uptake and efficient use of water and nutrients in saline soils. Research should also focus on the development of new products for cotton growing in saline soils like new foliar and specific slow-release fertilizers and commercial plant growth regulators to improve salt tolerance.

Ju Z.H.,Shandong Academy of Agricultural Sciences
Genetics and molecular research : GMR | Year: 2011

Transferrin (Tf) is a β-globulin protein that transports iron ions in mammalian cells. It contributes to innate immunity to microbial pathogens, primarily by limiting microbial access to iron. Thus, polymorphisms present in bovine Tf could potentially underlie inherited differences in mastitis resistance and milk production traits. We detected three novel single-nucleotide polymorphisms of the Tf gene in Chinese native cattle by screening for genetic variation of Tf in 751 individuals of three Chinese cattle breeds, namely China Holstein, Luxi Yellow and Bohai Black, using PCR-RFLP and DNA sequencing techniques. The three new SNPs, g.-1748G>A ss250608649, g.13942T>C ss250608650, and g.14037A>G ss250608651, had allele frequencies of 85.9, 86.3 and 92.5%, 64.5, 73.3 and 65.0%, and 67.6, 73.7 and 60.0%, respectively. SNP g.-1748G>A was located in the 5' flanking region of Tf. SNP g.14037A>G was located in intron 8 of Tf. SNP g.13942T>C, located in exon 8 of Tf, was a synonymous mutation (TTA > CTA), encoding a leucine (326 aa) in the Tf protein. Associations of the Tf SNPs with milk traits were also analyzed. Significant (P < 0.05) relationships among the Tf polymorphisms, somatic cell scores (SCS), and milk productive traits were observed. Cows with genotypes TT (g.13942T>C), GG (g.-1748G>A) and AG (g.14037A>G) had a lower SCS and higher protein levels and 305-day milk yield. Nineteen combinations of different haplotypes from the three SNPs were identified in Chinese Holstein cattle. The haplotype combination ATA/GCA, GCA/GCA and GCG/ GTA was dominant in cows with a lower SCS, a higher protein level and a higher 305-day milk yield, respectively. Moreover, the gene expression level of Tf was higher in mastitis-affected mammary tissues than in normal mammary tissues. These results suggest that the Tf gene affects milk production, as well as mastitis-resistance traits, in Chinese Holsteins.

Dong H.-Z.,Shandong Academy of Agricultural Sciences
Chinese Journal of Applied Ecology | Year: 2012

Stand establishment is the most difficult step for cotton planting on coastal saline-alkali soil. To establish and improve the techniques for stand establishment is the key in the production of high-yielding cotton on saline-alkali soil. Based on the previous studies and our own research progress in this field, this paper reviewed the effects and the underlying mechanisms of making unequal salt distribution in root zone, increasing soil moisture and temperature, establishing under-mulching greenhouse, and introducing seed coating agent in promoting stand establishment of cotton on saline-alkali soil. It was suggested that under the conditions of the average salt content in topsoil being not able to reduce, improving at least partial root zone environment through the induction of unequal salt distribution in the root zone and increasing soil moisture and temperature could significantly reduce salt injury and improve stand establishment. Flat seeding under plastic mulching on low-salinity soil, furrow seeding with mulching on moderate-or high-salinity soil, early mulching before sowing on rain-fed saline soil, and late sowing of short-season cotton in heat-limited area were the efficient techniques for improving the stand establishment of cotton on coastal saline-alkali soil. This review could provide full guarantee for the cotton stand establishment on coastal saline-alkali soil.

Shandong Academy of Agricultural Sciences | Date: 2013-08-19

A BrTCP24 gene for controlling the growth of Chinese cabbage and its applications. The BrTCP24 gene has the nucleotide sequence shown in SEQ ID No. 1, the whole length of which is 1221 bp. The gene is useful for improving the production of economic crop. As indicated by experimental results, the gene serves as a controlling gene for plant growth and has the function of down-regulating the plant growth. Through overexpressing the gene, a transgenic plant smaller than the wild one can be obtained. The gene of the invention can provide theory basis and gene source for breeding new varieties of crops.

Shandong Academy of Agricultural Sciences | Date: 2013-03-22

A method for producing laccase by liquid fermentation of Lentinus edodes comprises the following steps of: (1) inoculating strains of Lentinus edodes into the PD liquid fermentation medium to perform activation culture and obtain a seed liquid; (2) inoculating the seed solution into a liquid fermentation medium to perform liquid fermentation culture, then adding an expansin solution to perform further culture, separating the supernatant to obtain a ferment solution; (3) extracting laccase from the ferment solution. A plant expansin and an optimized fermentation method are applied for producing laccase by liquid fermentation of Lentinus edodes, which increases the liquid fermentation output of laccase of Lentinus edodes remarkably, by more than 3 times in comparing with traditional fermentation production methods.

Shandong Academy of Agricultural Sciences | Date: 2014-08-21

An application of thiolated single-stranded DNA enhances specific amplification of polymerase chain reaction, namely a method for enhancing specific amplification of polymerase chain reaction by utilizing thiolated single-stranded DNA, which includes the following step: adding an appropriate amount of the thiolated single-stranded DNA into a PCR system to perform PCR amplification, wherein the appropriate amount means that the final concentration of the thiolated single-stranded DNA in a 20 L reaction system is not less than 15 M. The thiolated single-stranded DNA meets the following conditions: the thiolated single-stranded DNA is one segment of any sequence which is non-complementary and non-homologous to a target sequence; the Tm value is not less than 37.7 C.; and at least one end contains a thiolalkyl group SHC_(6)H_(12).

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