Leng X.,Peking University |
Wu Y.,Peking University |
Wang X.,University of Southampton |
Pan Y.,Peking University |
And 10 more authors.
Journal of Human Genetics | Year: 2011
Vanishing white matter disease (VWM) is the first human hereditary disease known to be caused by defects in initiation of protein synthesis. Gene defects in each of the five subunits of eukaryotic translation initiation factor 2B (eIF2B α-ε) are responsible for the disease, although the mechanism of the pathogenesis is not well understood. In our previous study, four novel eIF2Bε mutations were found in Chinese patients: p.Asp62Val, p.Cys335Ser, p.Asn376Asp and p.Ser610-Asp613del. Functional analysis was performed on these mutations and the recently reported p.Arg269X. Our data showed that all resulted in a decrease in the guanine nucleotide exchange (GEF) activity of the eIF2B complex. p.Arg269X and p.Ser610-Asp613del mutants displayed the lowest activity, followed by p.Cys335Ser, p.Asn376Asp and p.Asp62Val. p.Arg269X and p.Ser610-Asp613del could not produce stable eIF2Bε, leading to almost complete loss-of-function. No evidence was obtained for the three missense mutations in changes in eIF2Bε protein level or eIF2BεSer 540 phosphorylation, and disruption of holocomplex assembly, or binding to eIF2. All patients in our study had the classical phenotype. p.Asp62Val and p.Asn376Asp mutations caused only mildly decreased GEF activity, were probably responsible for relatively mild phenotype in cases of classical VWM. © 2011 The Japan Society of Human Genetics All rights reserved.
Ze M.,Shan Xi Medical University |
Liu F.,Public Security of Yangquan City |
Lu J.,Shan Xi Medical University |
Guangmu R.,Shan Xi Medical University |
And 3 more authors.
Chinese Journal of Forensic Medicine | Year: 2016
Objective To explore the feasibility of inferring long PMI with HE staining and Pyronine-methyl green staining combined with image analysis technique. Methods The brain ,spleen and muscle tissues were collected from cases that the PMI had been determined. We extracted samples at 2d, 4d, 6d, 8d, 10d, 12d respectively. The average optical density of supporting tissues paraffin section was measured by using IPP5.0 microscopic image software. Experimental data were analyzed by Kruskal-Wallis test using SPSS13.0 software. Results Spleen and muscle showed a significant degradation on day 2, day 4 and day 6. The optical density of brain tissue showed unregularity fluctuation at all time points. The optical density of DNA/(RNA+impression) showed a significant degradation on day 2, day 4 and day 6 by Pyronine-methyl staining of brain tissues. Conclusion HE staining of spleen and muscle combined with pyronine-methyl green special staining of brain and image analysis technique can be used to infer PMI within one week, providing evidence for determination of late PMI.
Zhang K.,Shan Xi Medical University |
Zhang K.,Navy General Hospital |
Li J.-D.,Shan Xi Medical University |
Zhang R.-P.,Shan Xi Medical University |
And 2 more authors.
Chinese Journal of Radiology | Year: 2010
Objective: To develop a superparamagnetic iron oxide (SPIO)-based MR probe containing vascular endothelial growth factor(VEGF) to investigate their biological and chemical properties and targeting effect of colorectal cancer cells in vitro. Methods: The anti-VEGF-SPIO probe was fabricated with VEGF antibody and SPIO through chemical method. Its biological and chemical properties and reflexivity were tested with SDS-PAGE and MRI. The SW620 cells incubated with anti-VEGF-SPIO probe for 30, 60 and 90 minutes respectively and compared with marrow mesenchymal stem cell at 37°C. The comparison among groups was conducted by using analysis of variance and LSD-t test. The MRI results were confirmed by the Prussian blue staining. The comparison among groups was performed by analysis of variance and factorial experiment. Results: SPIO-based MR probe containing VEGF was successfully contributed and isolated. The reflexivity of anti-VEGF-SPIO probe was 0.0426 × 106 mol/s. The immunofluorescence and prussia blue stain proved high expression of VEGF in SW620 cells. Anti-VEGF-SPIO probe and SW620 cells combined at 37°C in vitro. MRI proved the SW620 cells incubated with anti-VEGF-SPIO probe appeared hypointense on T2 WI and T2 * WI. MR signal were 392 ± 7,91 ± 8,264 ± 10 for 30, 60 and 90 minutes respectively, which were statistically different from that before incubation 679 ± 12 (F = 4735.489, P < 0.01). The intensity decreased most significantly at 60 minutes in vitro. Its MR signal 82 ± 7 were statistically different compared with marrow mesenchymal stem cell 689 ± 43, t = 39.167, P < 0.05). While SW620 cells incubated without SPIO were not statistically different compared with marrow mesenchymal stem cell, which were 419 ± 59 and 400 ± 41 respectively (t = -0.718, P > 0.05). Conclusion: Nanoscale iron particles containing the anti-vascular endothelial growth factor molecular probe can evaluate tumor angiogenesis at the receptor level, which provides a new way of the tumor angiogenesis diagnosis and anti-angiogenesis therapy.
Lu X.,Shan xi Medical University |
Li X.,Shan xi Medical University
ICCASM 2010 - 2010 International Conference on Computer Application and System Modeling, Proceedings | Year: 2010
This paper proposes a Support Vector Machine integration methodology, which is based on dynamic selection method. Using FCM-SD algorithm, an improved method from FCM and closeness degree algorithms, the effective neighborhood of the discriminated sample is determined. Furthermore, based on the accuracies of segment classifications, a set of optimal individual classifiers are selected. Finally a weighted majority vote method is used to integrate the selected classifiers. Simulation results show that the proposed method reduces the complexity of the Integrated Classification model. It effectively improves the classification performance as well. © 2010 IEEE.
Kuai J.,Shan Xi Medical University |
Bao H.,Xuzhou Medical College |
Wang Y.,Shan Xi Medical University
Chinese Journal of Forensic Medicine | Year: 2014
Objective: To observe the effect of apelin-13 on traumatic brain injury (TBI), and explore the relationship between apelin-13 and autophagy in TBI. Methods: 30 mice were randomly divided into sham, saline and apelin-13 groups. Apelin-13 and saline groups were pretreated with an injection of apelin-13 (0.3 μg/g) and saline into the mouse brain lateral ventricle respectively 10 min before TBI. The autophagy-associated proteins LC3, P62, Beclin-1 and Bcl-2 in the mouse cerebral cortex were assessed by Western-Blot 24h and 48h after TBI. Results: Compared with sham group, protein LC3-11/LC3-I ratio and Beclin-1 expression were up-regulated, while protein Bcl-2 and P62 were down-regulated in saline group 24h or 48h after TBI. However, compared with saline group, Apelin-13 decreased protein LC3-II/LC3-I ratio and Beclin-1 expression, increased protein Bcl-2 and P62 expression, and down-regulated protein Beclin-1/Bcl-2 ratio 24h or 48h after TBI. Conclusion: Apelin-13 suppresses autophagy in TBI possibly by down-regulating protein Beclin-1/Bcl-2 ratio.