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Messripour M.,Islamic Azad University at Khorasgan | Mesripour A.,Shahre Kord University of Medical science
Biocell | Year: 2013

Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during 25 min was measured by HPLC, employing electrochemical detection. Dopamine synthesis in synaptosomes prepared from young animals was markedly inhibited by addition of 5 μM serotonin concentrations (30%) and increasing serotonin concentrations up to 50 μM caused only a smaller additional inhibition. Dopamine synthesis in synaptosomes obtained from old rats was significantly lower than that of youg animals and addition of serotonin concentrations up to 50 μM had little effect on these preparations. In case of serotonin synthesis, exogenously added 5 μM dopamine inhibited serotonin synthesis in the synaptosomes of both ages by about 40%, whereas with higher concentration of dopamine (10-50 μM) the rate of inhibition was highly pronounced in old rats as compared to that of young animals. It is concluded that dopamine and serotonin interaction may be significant, and that these should be considered in long-term treatments of Parkinson's disease with L-DOPA.

Nasri M.,Tarbiat Modares University | Karimi A.,Shahre Kord University of Medical science | Allahbakhshian Farsani M.,Shahid Beheshti University of Medical Sciences
Cytotechnology | Year: 2014

Viral vectors are valuable tools to deliver genetic materials into cells. Vectors derived from human immunodeficiency virus type 1 are being widely used for gene delivery, mainly because they are able to transduce both dividing and non-dividing cells which leads to stable and long term gene expression. In addition, these types of vectors are safe, with low toxicity, high stability and cell type specificity. Therefore, this work was aimed to produce lentivirus-based vector using a three-plasmid system. To produce this system, the eGFP marker gene was cloned into the plasmid pWPXLd. Subsequently, this vector plasmid, along with packaging plasmids, psPAX2 and envelope plasmid, pMD2.G, was co-transfected into packaging cell line (293T) using calcium phosphate method. 48 h post transfection, the constructed viral vector was harvested, purified and concentrated and stored at -80 °C for next experiments. The titration of the vector was carried out, using ELISA, flowcytometry, and fluorescent microscopy. Finally, transduction of HEK-293T, CHO, HepG2, MCF-7, MEFs and Jurkat cell lines was carried out with indicated cell numbers and multiplicities of infections of the vector in the presence of polybrene. Using this system, high titer lentivirus at titers of up to 2 × 108 transducing units/ml (TU/ml) was successfully generated and its transduction efficacy was improved by seven to over 20-fold in various cell types. We demonstrate the applicability of this vector for the efficient transduction of dividing and non-dividing cells, including HEK-293T, CHO, HepG2, MCF-7, MEFs and Jurkat cell line. Transduction efficiency yielded titers of (6.3 ± 1.2) 105 TU/ml. Furthermore, lentivirus transferred transgene was expressed at high level in the target cells and expression was followed until 90 days after transduction. Thus, the vector generated in this work, might be able to deliver the transgene into a wide range of mammalian cells. © 2014 Springer Science+Business Media Dordrecht.

Karimi A.,Shahre Kord University of Medical science | Mokhtarian K.,Shahre Kord University of Medical science
Kuwait Medical Journal | Year: 2010

Objective: To evaluate the level of anti-HBs antibody among health care workers (HCWs) in a university hospital in Shahre-Kord, Iran, during 2008-2009. Design: Prospective study. Setting: Charmahal-Baktiari province, Iran. Subjects: Two hundred and fifty seven health care workers (HCWs) in a university hospital. Intervention: Enzyme linked immunosorbent assay (ELISA). Main Outcome Measure: Seroprevalence of anti-HBsAg (IgG). Results: 85.6% of the individuals were female. Regardless of gender, 21 of the 257 (8.2%) HCWs lacked immunity, 91 of 257 (35.4%) were partially immune, and 145 (56.4%) exhibited immunity against the virus. The post-vaccination period was five years, in 221(86%) and more than five years in 36 of the 257 individuals studied (14%). There were more male non-responders (15%) than female (8%). There was a significant relationship between post-vaccination period and anti-HBsAg antibody titer (p < 0.05). Conclusion: Based on our results, the post-vaccination period of immunity to this virus in HCW workers is five years.

Keshavars M.,Tehran University of Medical Sciences | Kiani A.,Shahre Kord University of Medical science | Nasani L.,Tehran University of Medical Sciences | Hoseini A.F.,Tehran University of Medical Sciences
Koomesh | Year: 2012

Introduction: The effect of touch on growth of newborns has been achieved by educated staff, but touch therapy by mothers as the most important care provider for their infants, had not been focused in previous studies. The aim of this work was to study effect of touch therapy by mothers on weight gaining of preterm newborns. Materials and Methods: In a randomized controlled trail and double blind study, 60 preterm newborns who discharged from neonatal intensive care unit (NICU) to home, randomly allocated to control and touch therapy group (30 newborns in each group). Touch therapy was educated to mother in a direct education, also with offering guide booklet to the mothers. Touch was provided for three 20 minute period per day, until term corrected age. Neonate weight in two groups was measured at the time of discharge and then at the term corrected age by the electronic weighing scale with an accuracy of ± 10 gr. Results: In two groups, mean of age, kind of delivery, level of education, employment and socioeconomic status in mothers, also weight, sex and other neonate characteristics had no statistical differences. At the term corrected age, interventional group showed a weight gain of 10 g/day (30%) more than control, which was statistically significant (31 g/day vs 21 g/day) (P=0.04). Conclusion: An important aspect of this study is that all stimulations were provided by mothers which had positive impact for preterm newborns, So, education of touch therapy to mothers can be recommended before discharge of pre-term newborns.

Sharafati Chaleshtori F.,Shahre Kord University of Medical science | Taghizadeh M.,Kashan University of Medical Sciences | Rafieian-kopaei M.,Shahre Kord University of Medical science | Sharafati-chaleshtori R.,Kashan University of Medical Sciences
Journal of Food Processing and Preservation | Year: 2016

In this study, cumin and eucalyptus essential oils were prepared and analyzed by gas chromatography–flame ionization detector. Its antibacterial effects were screened using the microdilution method for Listeria monocytogenes, Salmonella typhi, Streptococcus pyogenes and Shigella dysenteriae. The effect of the three concentrations of essential oils (0.5, 1 and 2% w/v) with 2% chitosan at 4 ± 1C temperature and storage time of up to 9 days were evaluated on the microbial quality of chicken meat. Changes in total mesophilic bacterial count (TMBC), lactic acid bacteria, Enterobacteriaceae, mold and yeast counts, and sensory properties were evaluated. The main compounds in cumin were 1,8-cineol (26.75%) and cuminaldehyde (17.1%) and the main compounds in eucalyptus were 1,8-cineol (77.32%) and limonene (8.39%). The essential oils have antibacterial effects on the four examined bacteria. In all of the treatment groups, a decrease of TMBC up to the sixth day and lactic acid bacteria, Enterobacteriaceae, mold and yeast up to the ninth day (P < 0.05) were observed. Overall acceptance rate in the chicken meat containing chitosan with 0.5% cumin essential oil created a better sense. Hence, the use of chitosan combined with cumin and eucalyptus might be suggested as an antibacterial packaging to extend the shelf life and as a flavor enhancer for chicken meat. Practical Applications: The results suggested that because chitosan film incorporated with cumin and eucalyptus can reduce meat spoilage losses and improve nutritional value, it can be used as an active packaging in the meat industry. © 2015 Wiley Periodicals, Inc.

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