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Farhoudi A.,Tarbiat Modares University | Abedian Kenari A.M.,Tarbiat Modares University | Nazari R.M.,Shahid Rajaee Aquaculture Center | Makhdoomi Ch.,Shahid Rajaee Aquaculture Center
Iranian Journal of Fisheries Sciences | Year: 2013

This study was aimed to gain knowledge on the ontogeny of digestive enzymes in common carp larvae at the governmental Warm water Fish Aquaculture Center of Shahid Rajaee in Sari, Mazandaran, Iran. The ontogenetic development of pancreatic (trypsin, chymotrypsin, lipase and α-amylase) and intestinal (alkaline phosphatase and aminopeptidase-N) enzymes were assessed in common carp larvae from first feeding (3 days post hatching) to 33 days post hatching (dph). The larvae started to feed on rotifers at day 3 to day 7. Feeding on a commercial diet was started from day-8 onwards. Specific trypsin and chymotrypsin activities feed with rotifer were 0.011 U/mg protein and 0.003 U/mg protein at day 7, respectively; then the specific activities feed with dry food reached a maximum level at 33 dph (0.028 U/mg protein and 0.028 U/mg protein, respectively) (P<0.05) compared to live prey. The elevated alkaline proteases activity can be related to adaptation of larvae to digest protein content in the food. Specific lipase activity was 0.0006 U/mg protein at 7 dph; then the activity feed with dry food reached a maximum level at 15 dph (0.0011 U/mg protein) (P<0.05) compared to live prey. Specific α-amylase activity feed with rotifer was 85.86 U/mg protein on the 7 dph; consequently, its specific activity reached a maximum level at 15 dph (128.77 U/mg protein) (P<0.05). Specific alkaline phosphatase and aminopeptidase N activities feed with rotifer were 6 U/mg protein and 0.0106 U/mg protein at day 7, respectively; then the specific activities feed with dry food reached a maximum level at 33 dph (28.66 U/mg protein and 0.58 U/mg protein, respectively) (P<0.05) compared to live prey.


Farhoudi A.,Tarbiat Modares University | Abedian Kenari A.M.,Tarbiat Modares University | Nazari R.M.,Shahid Rajaee Aquaculture Center | Makhdoomi C.H.,Shahid Rajaee Aquaculture Center
Journal of Fisheries and Aquatic Science | Year: 2011

This study was aimed to gain knowledge on ontogeny of lipid and fatty acid profile in feral carp larvae and to determine nutritional requirements with a view to improving product quality. Real-time fatty acid profile was investigated in 1-33 day old larvae of Cyprinus carpio. This study was carried out at the governmental warm water fish aquaculture center of Shahid Rajaee in Sari, Mazandaran, Iran. Cyprinus carpio larvae obtained from breeders captured in an estuarine environment were analyzed for lipid/fatty acid composition. Feeding with rotifer started from day 3 to day 7 and then larvae were fed with dry diet from day 8 onwards. Decreased total lipid and fatty acid profile alterations indicated energy-directed preferential metabolism of studied nutrients within certain intervals. During early larval stages, reduced monounsaturated fatty acids showed that these fatty acids were utilized as an energy source (p<0.05). Increased contents of docosahexaenoic acid and eicosapentaenoic acid well proved that these polyunsaturated fatty acids were not energy-generating infstrates (p<0.05) instead, were preserved as structural components for physiological processes. Marine carp larvae apparently metabolized dietary linolenic acid to eicosapentaenoic acid and docosahexaenoic acid and dietary linoleic acid to Arachidonic acid. Arachidonic acid was clearly insignificantly utilized (p>0.05). The percentage of body protein andash increased with body weight but percentage of body lipid and moisture decreased. © 2011 Academic Journals Inc.


Khosravi Bakhtiarvandi N.,Tarbiat Modares University | Abedian Kenari A.,Tarbiat Modares University | Mohammad Nazari R.,Shahid Rajaee Aquaculture Center | Makhdoomi C.,Shahid Rajaee Aquaculture Center
Iranian Journal of Fisheries Sciences | Year: 2014

Body composition and fatty acid profile of the body tissue of Caspian kutum (Rutilus frisii kutum) fish larvae were determined from fertilized egg to 50 days post hatching (dph). Feeding with rotifer started from day 3 to day 7; thereafter they were fed with dry food until the end of the experiment. The changes observed in lipid content and the decrease in lipid levels during larval ontogeny reflect the utilization and mobilization of lipids during both embryogenesis and larvae development. During this study, palmitic and stearic acids were the most abundant saturated fatty acid (SAFA).In kutum larvae, no utilization of palmitic and stearic acids was observed until 20 dph. It thereafter was utilized as an energy source. Oleic and palmitoleic cacids were the predominant monounsaturated fatty acid (MUFA). Oleic and palmitoleic acids utilized until 10 and 25 dph, respectively; thereafter they increased. DHA, EPA and ARA were the major polyunsaturated fatty acids (PUFA). The decrease in levels of DHA and EPA denotes their utilization as an energy substrate by kutum larvae. ARA decreased during larval ontogeny, reflecting dietary values. With regard to reared kutum larvae in fresh water and the low levels of HUFA in larval diet, it can be said that kutum larvae possibly are quite capable of elongating and desaturating C18 to C20 PUFA. In case of proximate analysis, the percentage of body protein and ash increased trend during ontogeny while the percentage of body lipid and moisture decreased trend during larval growth.

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