Singh R.,Yale University |
De Aguiar R.B.,Yale University |
Naik S.,Yale University |
Mani S.,Yale University |
And 6 more authors.
Cell Metabolism | Year: 2013
Common genetic variations in Wnt signaling genes have been associated with metabolic syndrome and diabetes by mechanisms that are poorly understood. A rare nonconservative mutation in Wnt coreceptor LRP6 (LRP6R611C) has been shown to underlie autosomal dominant early onset coronary artery disease, type 2 diabetes, and metabolic syndrome. We examined the interplay between Wnt and insulin signaling pathways in skeletal muscle and skin fibroblasts of healthy nondiabetic LRP6R611C mutation carriers. LRP6 mutation carriers exhibited hyperinsulinemia and reduced insulin sensitivity compared to noncarrier relatives in response to oral glucose ingestion, which correlated with a significant decline in tissue expression of the insulin receptor and insulin signaling activity. Further investigations showed that the LRP6 R611C mutation diminishes TCF7L2-dependent transcription of the IR while it increases the stability of IGFR and enhances mTORC1 activity. These findings identify the Wnt/LRP6/TCF7L2 axis as a regulator of glucose metabolism and a potential therapeutic target for insulin resistance. © 2013 Elsevier Inc. Source
Oryan G.,Isfahan University of Medical Sciences |
Faghri J.,Isfahan University of Medical Sciences |
Fazeli H.,Isfahan University of Medical Sciences |
Zandi A.,Isfahan University of Medical Sciences |
And 3 more authors.
Journal of Isfahan Medical School | Year: 2014
Background: This study aimed to determine the prevalence and antibacterial resistance of coagulase negative staphylococci in keratitis infections after using soft contact lenses in patients admitted at Feiz hospital, Isfahan Iran, in 2013. Methods: In this study, 77 patients with keratitis were examined. The samples were cultured and isolation of coagulase negative staphylococcus was done using phenotypic tests and in-vitro sensitivity testing was done using Kirby-Bauer disc diffusion susceptibility method. Findings: 38 isolates were conveniently identified as coagulase negative staphylococci. 27 samples (71.1%) were resistant to penicillin, 21 (55.3%) to erythromycin, and 16 (42.1%), to tetracycline. All the isolates (100%) were sensitive to gentamicin, 36 isolates (94.7%) were sensitive to chloramphenicol, and 33 (86.8%) to ciprofloxacin. Resistance to cefoxitin was seen in 7 (18.4%). Conclusion: Results of this study showed that coagulase negative staphylococci were the most common agents causing contact-lens-related microbial keratitis. In addition, gentamycin, chloramphenicol and ciprofloxacin were more suitable than other antibacterial agents against this type of bacteria. Source
Rashidi Pour Fard N.,Shahid Motahari Hospital |
Karimi M.,Shahid Motahari Hospital |
Baghaei M.H.,Shahid Motahari Hospital |
Haghighatdoost F.,Isfahan University of Medical Sciences |
And 3 more authors.
ARYA Atherosclerosis | Year: 2015
BACKGROUND: Previous epidemiological studies of dairy product consumption and health outcomes have reported mixed findings. Despite increasing in life expectancy, scarce data are available in this field in elderly individuals. We tested the hypothesis that greater dairy intake is associated with lower high sensitive C-reactive protein (hs-CRP) level and better lipid profile and glycemic control. METHODS: This cross-sectional study was undertaken on 107 elderly individuals who aged 60-78 years. Usual dietary intakes were assessed by means of a validated food frequency questionnaire (FFQ). Anthropometric measures and biochemical markers were determined using standard protocols. RESULTS: The reported mean ± standard deviation (SD) of daily intake of dairy products and age were 588.02 ± 418.88 g/d and 63.22 ± 6.92 years, respectively. After control for demographic characteristics and dietary intakes, dairy consumption was not significantly related to the increased risk of insulin resistance [Odds ratio (OR): 2.19, 95% confidence interval (CI): 0.54, 8.86; P = 0.520] and elevated hs-CRP (OR: 1.54, 95% CI: 0.37, 6.35; P = 0.550). Participants in the top tertile of dairy had greater, but statistically not a significant risk of elevated triglyceride (TG), total cholesterol and low-density lipoprotein cholesterol (LDL-C). No significant relations were seen for hs-CRP, insulin resistance and lipid profile across tertiles of dairy products. CONCLUSION: In this elderly population, total dairy consumption was not associated with inflammatory biomarkers levels and other cardiometabolic risk factors. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved. Source