Chen J.,Shaanxi Tumour Hospital |
Zhao W.-H.,Shaanxi Tumour Hospital |
Song Z.-J.,Shaanxi Tumour Hospital |
Chen H.-G.,Tianjin Medical University |
And 3 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2014
Objective: To investigate the effects of propofol on the proliferation and apoptosis of lung cancer cells as well as the related molecular mechanisms. Methods: HCC827 cells were seeded in well plates with a density of 1×106 and then randomly divided into 5 groups: control group (group C), intralipid group (group E), low-dose propofol group (1.5 μL/m L, group P1), medium-dose propofol group (2.2 μL/m L, group P2), and high-dose propofol group (3.2 μL/mL, group P3). At 6 h, 24 h and 48 h after propofol treatment, the cells were collected to detect their proliferation and apoptosis. At 6h after treatment, the cells were collected for the measurement of Nrf2 mRNA and protein by RT-PCR and Western blot. Results: Cell inhibition rate (IR) and apoptosis as well as Nrf2 mRNA and protein expressions in group E did not differ significantly from those in group C (P>0.05). Compared with those in groups C and E, IR and apoptosis and Nrf2 mRNA and protein expressions were significantly increased in groups P1, P2 and P3 (P<0.05). Conclusion: Propofol can inhibit the proliferation of cancer cells and promote cell apoptosis, thereby inhibiting the reoccurrence and metastasis of cancer cells probably via regulating the activation of Nrf2 expression.