Fengcheng, China
Fengcheng, China

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Dong Y.,Xi'an Jiaotong University | Zeng W.-H.,Xi'an Jiaotong University | Li W.-B.,Shaanxi TCM Hospital | Zheng W.,Xi'an Jiaotong University
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2013

Objective: To evaluate the effects of narrow band-UVB (NB-UVB) combining tacrolimus on melanogenesis and tyrosinase activities in human melanocytes. Methods: MTT assay was performed to select the optimal concentration of tacrolimus. Melanocytes were classified into tacrolimus group, NB-UVB group and NB-UVB combining tacrolimus group, respectively; those receiving no treatment served as controls. Tyrosinase activities were evaluated with L-dopa as the substrate. Melanin content was detected by NaOH assay. Results: There was no significant difference in proliferation of melanocytes in tacrolimus group (10, 102, 103 μmol/L) compared with the control group (P≤0.05), and tacrolimus (104 μmol/L) decreased the proliferation of melanocytes markedly compared with the control group (P<0.05). Therefore, the optimal concentration was 103 μmol/L. Tyrosinase activities and melanin content increased significantly in tacrolimus group, NB-UVB group, and NB-UVB combining tacrolimus group compared with the control group (P<0.05). Tyrosinase activities and melanin content increased more markedly in NB-UVB combining tacrolimus group than in tacrolimus group or NB-UVB group (P<0.05). Conclusion: Tacrolimus combining NB-UVB treatment can synergistically enhance activities of tyrosinase and accelerate melanogenesis.


Li W.-B.,Shaanxi TCM Hospital | Dong Y.,Xi'an Jiaotong University | Yan X.-N.,Shaanxi TCM Hospital | Li M.-H.,Shaanxi TCM Hospital | And 2 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2014

Objective: To evaluate the effects of tacrolimus on intercellular adhesion molecule-1 (ICAM-1), MMP-2 and MMP-9 expressions in human melanocytes.Methods: In the experiment melanocytes were divide into normal control group and tacrolimus groups (10, 102, 103 and 104 nmol/L). Q-RT-PCR and Western blotting were performed to evaluate the effects of tacrolimus on ICAM-1 expression induced by TNF-α(10 ng/mL) and on MMP-2 and MMP-9 expressions in melanocytes.Results: Compared with that in control group, the expression of ICAM-1 was suppressed markedly in all the tacrolimus groups (P<0.05, P<0.01). However, the expressions of MMP-2 and MMP-9 were increased in all the tacrolimus groups (P<0.05, P<0.01).Conclusion: Topical tacrolimus can be used as an effective agent to treat vitiligo, maybe due to its inhibiting ICAM-1 expression and inducing MMP-2 and MMP-9 expressions, which can reduce immunologic injury and facilitate migration of menalocytes.


Dong Y.,Xi'an Jiaotong University | Li W.-B.,Shaanxi TCM Hospital | Zheng W.,Xi'an Jiaotong University | Wang J.,Xi'an Jiaotong University | And 2 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2016

Objective: To investigate the effect of narrow-band UVB (NB-UVB) on melanocytes proliferation, melanin production, tyrosinase activities and miRNA-25 expression so as to explore the relationship between NB-UVB and miRNA-25 and the possible mechanism of NB-UVB for treatment of vitiligo. Methods: Melanocytes cultured in vitro were treated with 40 mJ/cm2 dose of NB-UVB for 72 h, the effects of NB-UVB on cell proliferation, tyrosinase activity and melanin content were investigated. After NB-UVB stimulation for 12 h, the effect of NB-UVB on miRNA-25 expression in melanocytes was detected. Melanocytes were transfected with miRNA-25 mimics, miRNA-25 inhibitor and miRNA-25 mutant, respectively, the changes of cell proliferation, tyrosinase activitiy and melanin content were observed. Cell viability was detected using MTT method. Tyrosinase activities were measured with levodopa as the substrate. NaOH assay was used for the detection of melanin content. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect miRNA-25 expression. Results: After NB-UVB of 40 mJ/cm2 dose was used for the cells cultured for 72 h, the viability of melanocytes, tyrosinase activities, and melanin content were significantly increased (P<0.05). After NB-UVB stimulation for 12 h, miRNA-25 expression was significantly lower than that in the control group (P<0.05). After knockdown of miRNA-25, the cell proliferation of melanocytes, tyrosinase activities and melanin content were increased, whereas overexpression of miRNA-25 decreased the cell proliferation, tyrosinase activities and melanin production. Overexpression of miRNA-25 partially inhibited the effect of NB-UVB on the treatment of melanocytes. Conclusion: NB-UVB may promote cell proliferation, increase tyrosinase activities and melanin formation through partially downregulating the expression of miRNA-25 in melanocytes. © 2016, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.


Yan X.N.,Shaanxi TCM Hospital
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

To assess the clinical efficacy and safety of surrounding needle, moxibustion and hot compress of TCM herbs for localized scleroderma. Forty-two cases of localized scleroderma were randomly divided into an acupuncture + herb group (23 cases, group A) and a heparin sodium group (19 cases, group B). Both the two groups were orally administrated with centella triterpenes tablets and vitamin E, group A was additionally treated with surrounding needle at local area, moxibustion at affected site and Hegu (LI 4), Zu sanli (ST 36) as well as hot external application of "hot compress herbs" at local location, while group B was treated with external application of heparin sodium cream. Both the two groups were treated for consecutive 6 months, and scores of skin sclerosis, joint pain and function were compared before and after the treatment. Also the efficacy and safety of TCM syndrome were assessed. Compared with that before the treatment, the scores of skin sclerosis, joint pain and joint function in the group A after treatment were significantly decreased (all P < 0.01), the score of skin sclerosis in the group B was improved (P < 0.05), and the three types of score in the group A was obviously lower than those in the group B (both P < 0.05). The total effective rate was 86.4% (19/22) in the group A, which was superior to 52.6% (10/19) in the group B (P < 0.05). The surrounding needle, moxibustion and external application of "hot compress herbs" could improve skin sclerosis in patients with localized scleroderma, which has obvious efficacy and relative safety.

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