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Wei X.C.,Shaanxi Provincial People Hospital
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology

This study was aimed to investigate the effect of cord blood dendritic cells (DCs) on the in vitro proliferation capability, immunophenotype changes, level of secreted cytokines and activity against leukemia cells of the homologous cytokine-induced killer (CIK) cells. DCs and CIK cells were induced from cord blood mononuclear cells. They were co-cultured at the ratio of 1:5, and CIK cells from cord blood or DC-CIK cells from peripheral blood were cultured as controls. Immunophenotypic changes were analyzed by flow cytometry, increased number of cells were counted by trypan-blue staining, the killing activity to leukemia cells was assayed by MTT, the levels of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-12 (IL-12) in the cultured supernatant were detected by ELISA. The results showed that the proliferation capability of cord blood DC-CIK cells was significantly higher than that of cord blood CIK cells and peripheral blood DC-CIK cells (p < 0.05 and p < 0.05). Under the same condition, the rate of double positive cells with CD3(+)CD8(+) and CD3(+)CD56(+) in CIK cells was significantly enhanced by co-culture with cord blood DCs (p < 0.05). The level of IL-12, IFN-γ, and TNF-α in cultured supernatants of cord blood DC-CIK cells increased noticeably on day 3 as compared with CIK cells cultured alone (p < 0.01, p < 0.05, p < 0.05). Within the effector-target ratio range between 2.5:1 to 20:1, the activity of cord blood DC-CIK cells against all subtypes of acute leukemia cells was much higher than that of CIK cells (p < 0.05), and there was no significant difference among all subtypes of acute leukemia cells, which was the same with the killing effect of peripheral blood DC-CIK cells against leukemia cells. It is concluded that the proliferation capability and anti-leukemia effect of the homologous CIK cells can be enhanced by cord blood DCs. The proliferation capability of cord blood DC-CIK cells is stronger than that of peripheral blood DC-CIK cells, but there is no significant differences of cytotoxicity between DCs and CIK cells. As the cord blood is easily gained and does not easily cause a serious graft rejection, the DC-CIK cells should be clinically applied more extensively as novel immune therapy. Source

Bai G.,Xian Jiaotong University | Wang Y.,Xian Jiaotong University | Zhang L.,Shaanxi Provincial People Hospital | Tang Y.,Xian Jiaotong University | Fu F.,Xian Jiaotong University
Archives of Gynecology and Obstetrics

Objective This study aims to understand the difference of HBxAg and PI3K signal transduction protein expressions in HBV-infected placenta and normal placenta, clarify the difference of the two in the degree of apoptosis and explore the potential role of inhibition of HBxAg/PI3 K/apoptosis in HBV intrauterine infection. Methods Placenta tissues of 24 pregnant women with confirmed intrauterine infection and positive HBsAg were selected as the infection group, and those of normal healthy pregnant women were taken as the control group. Immunohistochemical SP staining method was employed to detect the expressions of HBxAg and PI3K in the placenta of each group, and TUNEL was applied for the assay of apoptosis. Results HBxAg was detected in the placenta of HBVinfected group, and staining optical density value of high replication group (HBV DNA [1 9 103 copies/mL) was higher than that of low replication group (HBV DNA \1 9 103 copies/mL), and there was statistical significance (p\0.05); PI3K expression levels in the placenta of HBV-infected groups were higher than that of the control group and there was statistical significance (p\0.01), and staining optical density value of high replication group was higher than that of low replication group and it was statistically significant (p\0.01); apoptosis index of HBVinfected high replication group was lower than that of low replication group and control group and there was statistical significance (p\0.01). Conclusion HBV infected placenta tissues and then produced functional proteins HBxAg in trophoblast cells, and HBxAg/PI3 K/anti-apoptosis is the potential mechanism for pregnant women with HBV DNA high replication to have intrauterine infection while there exists different mechanism for pregnant women with negative HBV DNA. © Springer-Verlag 2011. Source

Liang X.-Y.,Shaanxi Provincial People Hospital | Ma S.-J.,Shaanxi Provincial People Hospital | Wang Y.,Shaanxi Provincial People Hospital | Kang X.-L.,Shaanxi Provincial People Hospital | And 3 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences)

Objective: To explore the expressions of Runx2 and osteopontin (OPN) in human breast cancer tissue and their association with the formation of microcalcification.Methods: The expressions of Runx2 and OPN were detected in breast cancer tissue of 62 cases by immunohistochemical method. Then the 62 cases were divided into three groups: non-microcalcification, mild-microcalcification and high-microcalcification groups according to the amount of microcalcification shown on mammography in breast cancer. The association of Runx2 and OPN expressions with microcalcification was further studied.Results: The positive expression rate of Runx2 and OPN in human breast cancer tissue was 72.6% and 79.0%, respectively. The positive expression of Runx2 and OPN proteins was significantly correlated with the amount of microcalcification, but not with the morphology of microcalcification in breast cancer tissue. The positive rate of Runx2 and OPN was gradually elevated with the increase in the amount of microcalcification (χ2=15.686, P<0.05; χ2=16.161, P<0.05), respectively.Conclusion: Runx2 and OPN proteins are highly expressed in human breast cancer tissue and they may participate in the process of microcalcification formation in breast cancer. ©, 2014, Xi'an Medical University. All right reserved. Source

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