Shaanxi Provincial General Hospital of Chinese Peoples Armed Police Force

Fengcheng, China

Shaanxi Provincial General Hospital of Chinese Peoples Armed Police Force

Fengcheng, China
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Da C.,Shaanxi Provincial General Hospital of Chinese Peoples Armed Police Force | Gu Y.,Shaanxi Provincial General Hospital of Chinese Peoples Armed Police Force | Duan W.,Shaanxi Provincial General Hospital of Chinese Peoples Armed Police Force | Wu X.,Shaanxi Provincial General Hospital of Chinese Peoples Armed Police Force
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To explore the effect of the down-regulation of Medl9 expression by RNAi on the proliferation and apoptosis of colon cancer Caco-2 cells. Methods: The pSilencer-Medl9-siRNA interference plasmid targeting Medl9 was constructed, which was transfected into Caco-2 cells. The level of Medl9 mRNA in transfected Caco-2 cells was detected by RT-PCR and the level of Medl9 protein was determined by Western blotting. Flow cytometry and MTT were performed to detect the proliferation and apoptosis of Caco-2 cells after pSilencer-Medl9-siRNA transfection. Results: The results of RT-PCR and Western blotting showed that the mRNA and protein levels of Medl9 declined markedly in Caco-2 cells that were transfected by pSilencer-Medl9-siRNA vector (P < 0. 01). Compared with the control pSilencer group, flow cytometry and MTT assay demonstrated that pSilencer-Medl9-siRNA significantly suppressed proliferation (7 d: [0. 86 ±0. 09]% vs [1. 38 ± 1. 10]%, P < 0. 01) and induced the apoptosis of Caco-2 cells ([22. 72 ± 2. 85]% vs [7. 23 ± 1. 29]%, P <0. 01) in the pSilencer-Medl9-siRNA group. Conclusion: Silence Medl9 expression by interference RNA can inhibit the proliferation and induce apoptosis of colon cancer Caco-2 cells, so Medl9 may act as a potential therapeutic target in colon cancer.

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