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Zheng J.,Chinese Peoples Liberation Army | Zheng J.,PLA Fourth Military Medical University | Ma L.,PLA Fourth Military Medical University | Ren Q.,PLA Fourth Military Medical University | And 9 more authors.
BMC Gastroenterology | Year: 2014

Background: Activated hepatic stellate cells are the main source of excessive collagen deposition in liver fibrosis. Here we report the inhibitory effects of the combinational treatment of two natural products, astragalus polysaccharide (APS) and β-elemene (ELE) on the activation of human liver hepatic stellate cell line LX-2 cells. Methods: Cultured LX-2 cells were treated with different concentrations of APS or ELE for 24 or 48 hours. Cell viability/apoptosis was measured by MTT assay and Annexin V/PI staining , activation related genes including α-SMA and CD44 expressions were measured by real-time PCR and western blot respectively. Results: The majority of LX-2 cells showed morphological change in the presence of APS or ELE for 24 hours. Treatment with APS + ELE for 24 or 48 hours significantly inhabited the cell proliferation compared with APS or ELE treatment alone on LX-2 cells. APS + ELE may block the up-regulation of α-SMA and CD44 both in mRNA and protein levels through TGF-β pathway in LX-2 cells. Conclusion: APS or ELE treatment alone on LX-2 cells could inhibit cell proliferation and induce apoptosis. The combinational treatment using APS + ELE significantly increased the killing efficiency on LX-2 cells. α-SMA and CD44 expressions was inhibited upon APS + ELE treatment through TGF-β pathway in LX-2 cells. The results indicated a novel treatment using natural products for liver diseases with anti-fibrotic effect.


Wang J.-W.,Shaanxi Province Hospital of Traditional Chinese Medicine | Dang J.-J.,Shaanxi Province Hospital of Traditional Chinese Medicine | Li Q.,Shaanxi Province Hospital of Traditional Chinese Medicine | Li W.-G.,Shaanxi Province Hospital of Traditional Chinese Medicine | Zheng Y.,Shaanxi Province Hospital of Traditional Chinese Medicine
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Tissue engineering scaffolds are designed to imitate extracellular matrix, which is essential for cellular growth and metabolism. The scaffold preparation and the selection of seed cells are the hot topics in bone tissue engineering field. Objective: To construct three-dimensional growth environment of adipose-derived stem cells with the chitosan/xenogeneic bone, and investigate its compatibility. Methods: Adipose tissue was obtained from New Zealand white rabbits at 8 days old, and adipose-derived stem cells were extracted. After induction of osteogenic differentiation in vitro, the cells were cultured on chitosan/xenogenic bone, to construct bone tissue engineering complex, serving as the experimental group. The adipose-derived stem cells were directly cultured in xenogenic bone, to construct cells/xenogeneic bone complex, serving as a control group. The simple bone xenograft served as a blank group. Two weeks after in vitro induced differentiation, the complex or xenograft in three groups were observed under scanning electron microscope to observe the cells and scaffold compound the situation. Results And Conclusion: The chitosan fully penetrated into the scaffolds, and formed a three-dimensional environment for cell growth, so that adipose-derived stem cells can grow in the three-dimensional environment, which provide sufficient space for the regeneration of the extracellular matrix. After induction of the chitosan/xenogenic bone, adipose-derived stem cells can carry more cells, and the loss of cells in the vector is reduced, so it is a good carrier of bone tissue engineering.

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