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Li Z.,Xi'an Jiaotong University | Ren J.,Xi'an Jiaotong University | Niu X.,Xi'an Jiaotong University | Xu Q.,Traditional Chinese Medicine Hospital of Shaanxi Province | And 3 more authors.
BioMed Research International | Year: 2016

Objective. The objective of this study was to systematically evaluate the association between vitiligo and human leukocyte antigen- (HLA-) A. Methods. PubMed, Embase, Web of Science, Chinese National Knowledge Infrastructure, and reference lists were searched for relevant original articles. Results. Nineteen case-control studies comprising 3042 patients and 5614 controls were included, in which 33 HLA-A alleles were reported. Overall, three alleles (HLA- A 02, A 33, and A w 31) were significantly associated with increased risk of vitiligo, two (HLA- A 09 and A w 19) were associated with decreased risk, and the remaining 28 were unassociated. Twelve alleles, seven alleles, and 19 alleles were common to three ethnicities, both types of vitiligo, and both typing methods, respectively. In the subgroup analysis by ethnicity and typing methods, the association of six alleles and five alleles was inconsistent in three populations and both typing methods, respectively. In the subgroup analysis by clinical type, the association of all seven alleles was consistent in both types of vitiligo. Conclusion. The meta-analysis suggests that HLA- A 02, A 33, and A w 31 are associated with increased risk of vitiligo, while HLA- A 09 and A w 19 are associated with decreased risk of vitiligo. The association of some alleles varies in terms of ethnicity and typing methods. © 2016 Zhangjun Li et al.


Wang N.,PLA Fourth Military Medical University | Wang H.,PLA Fourth Military Medical University | Chen J.,Traditional Chinese Medicine Hospital of Shaanxi Province | Zhang X.,PLA Fourth Military Medical University | And 5 more authors.
Cytotechnology | Year: 2014

Multi-differentiation capability is an essential characteristic of bone marrow mesenchymal stem cells (BMSCs). Method on obtaining higher-quality stem cells with an improved differentiation potential has gained significant attention for the treatment of clinical diseases and developmental biology. In our study, we investigated the multipotential differentiation capacity of BMSCs under simulated microgravity (SMG) condition. F-actin staining found that cytoskeleton took on a time-dependent change under SMG condition, which caused spindle to round morphological change of the cultured cells. Quantitative PCR and Western Blotting showed the pluripotency marker OCT4 was up-regulated in the SMG condition especially after SMG of 72 h, which we observed would be the most appropriate SMG duration for enhancing pluripotency of BMSCs. After dividing BMSCs into normal gravity (NG) group and SMG group, we induced them respectively in endothelium oriented, adipogenic and neuronal induction media. Immunostaining and Western Blotting found that endothelium oriented differentiated BMSCs expressed higher VWF and CD31 in the SMG group than in the NG group. The neuron-like cells derived from BMSCs in the SMG group also expressed higher level of MAP2 and NF-H. Furthermore, the quantity of induced adipocytes increased in the SMG group compared to the NG group shown by Oil Red O staining, The expression of PPARγ2 increased significantly under SMG condition. Therefore, we demonstrated that SMG could promote BMSCs to differentiate into many kinds of cells and predicted that enhanced multi-potential differentiation capacity response in BMSCs following SMG might be relevant to the changes of cytoskeleton and the stem cell marker OCT4. © 2013 Springer Science+Business Media Dordrecht.

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