Shaanxi Province Academy of science

Fengcheng, China

Shaanxi Province Academy of science

Fengcheng, China
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Wang Y.,Xi'an University of Technology | Wang Y.,Shaanxi Province Academy of science | Nie M.,Xi'an University of Technology | Wang F.,Xi'an University of Technology | And 2 more authors.
Xi'an Jianzhu Keji Daxue Xuebao/Journal of Xi'an University of Architecture and Technology | Year: 2017

l-hydroxy-2-naphthoic acid was identified as one kind of OPAHs that was most easily accumulated during the process of bioremediation. It was toxic to almost all microorganisms, and inhibited successfully restoring PAHs polluted environment. In this study, P. stutzeri N2, which was selected for its special ability in our previous research, was taken as the object. The capability and characterization of l-hydroxy-2-naphthoic acid degradation by P. stutzeri N2 was studied. At the same time, the intermediate metabolisms of l-hydroxy-2-naphthoic acid degraded by P. stutzeri N2 were identified by GC-MS method. The results showed that P. stutzeri N2 could utilize 50 mg/L l-hydroxy-2-naphthoic acid as the sole carbon and energy resource. When octane was used as the co-carbon source, the growth increase and degradation rate of P. stutzeri N2 could be improved by 20%. Based on the analysis of nine degradation metabolites by P. stutzeri N2, decarboxylation was proposed as the main degradation pathway. This study can help lay a foundation for the problem of high-toxic OPAHs accumulation during the process of bioremediation of PAHs polluted environment. © 2017, Editorial Office of Journal of Xi'an University of Architecture & Technology. All right reserved.


Zhang Y.,Shaanxi Province Academy of science | Zhang K.,Shaanxi Province Academy of science | Wan Y.,Shaanxi Province Academy of science | Zi J.,Shaanxi Province Academy of science | And 4 more authors.
Biotechnology Progress | Year: 2015

Human epidermal growth factor (hEGF) is a cellular factor that promotes cell proliferation and has been widely used for the treatment of wounds, corneal injuries, and gastric ulcers. Recombinant hEGF (rhEGF) has previously been expressed using the pTWIN1 system with pH-induced intein and a chitin-binding domain. The rhEGF protein can be purified by chitin affinity chromatography because of the high affinity between the chitin-binding domain fusion-tag and the column. However, uncontrolled cleavage presents a major problem with this method. To overcome this problem, a novel purification method has been developed for a pH-induced intein tag rhEGF that is expressed in Escherichia coli. Following purification by denaturation of inclusion bodies, the fusion protein is renatured and simultaneously induced to self-cleave by dialysis. Further purification of rhEGF is achieved by heat treatment and ion-exchange chromatography. Our results show that the purity of rhEGF obtained through this method is over 98% and the quantity of purified rhEGF is 248 mg from a 1 L culture or 2,967 mg from a 12 L culture. Therefore, we conclude that we have developed an efficient purification method of rhEGF, which may be used for the purification of other heat-resistant and acid-resistant recombinant proteins. © 2015 American Institute of Chemical Engineers.


PubMed | Shaanxi Province Academy of science, Northwest University, China and PLA Fourth Military Medical University
Type: Journal Article | Journal: Biotechnology progress | Year: 2015

Human epidermal growth factor (hEGF) is a cellular factor that promotes cell proliferation and has been widely used for the treatment of wounds, corneal injuries, and gastric ulcers. Recombinant hEGF (rhEGF) has previously been expressed using the pTWIN1 system with pH-induced intein and a chitin-binding domain. The rhEGF protein can be purified by chitin affinity chromatography because of the high affinity between the chitin-binding domain fusion-tag and the column. However, uncontrolled cleavage presents a major problem with this method. To overcome this problem, a novel purification method has been developed for a pH-induced intein tag rhEGF that is expressed in Escherichia coli. Following purification by denaturation of inclusion bodies, the fusion protein is renatured and simultaneously induced to self-cleave by dialysis. Further purification of rhEGF is achieved by heat treatment and ion-exchange chromatography. Our results show that the purity of rhEGF obtained through this method is over 98% and the quantity of purified rhEGF is 248 mg from a 1 L culture or 2,967 mg from a 12 L culture. Therefore, we conclude that we have developed an efficient purification method of rhEGF, which may be used for the purification of other heat-resistant and acid-resistant recombinant proteins.


Lu Q.-Q.,Institute of Botany of Shaanxi Province | Shi X.-W.,Institute of Botany of Shaanxi Province | Yang X.-K.,Shaanxi Province Academy of science
Acta Crystallographica Section E: Crystallographic Communications | Year: 2015

The title compound, C15H22O3·H2O, is a natural producr isolated from Chloranthus japonicus, which is a eudesmane sesquiterpenoid. The two trans-fused six-membered rings have chair confomations. In the crystal, O-H⋯O hydrogen bonds link the components into corrugated layers parallel to the bc plane. There are C-H⋯O interactions present within and between the layers.


Nie H.,Shaanxi University of Technology | Nie M.,Shaanxi University of Technology | Yang Y.,Shaanxi University of Technology | Zhao J.,Shaanxi University of Technology | And 4 more authors.
Polycyclic Aromatic Compounds | Year: 2015

Characterization of phenol metabolization by P. stutzeri N2 was proposed based on the identification of relevant metabolites and enzyme activities of catechol 1,2-oxygenase and catechol 2,3-dioxygenase. P. stutzeri N2 started to grow on 400 mg l−1 phenol with no obvious lag phase, and then phenol decomposition was rapidly completed within 32 h. Results of enzyme activities, UV spectrums of cultures, and the mass spectrum identification of cis, cis-muconic and 2-HMS, which showed that catechol C12O and C23O were acting simultaneously on the fission of the phenol ring. The existence of P-hydroxybenzoic acid and straight-chain saturated dicarboxylic acids in the culture medium showed that carboxylation and hydrogenation were involved during the phenol degradation. Metabolites containing five or four carbons indicated that catechol and its immediate ring-cleavage intermediates could be furthermore metabolized by P. Stutzeri N2. Therefore, P. stutzeri N2 which could degrade phenol with high efficiency and diverse pathways could have potential applications in the treatment of wastewater containing phenol. 2015 Copyright © Taylor & Francis Group, LLC


Nie M.,Shaanxi University of Technology | Nie H.,Shaanxi University of Technology | Cao W.,Shaanxi University of Technology | Wang X.,Shaanxi University of Technology | And 4 more authors.
Polycyclic Aromatic Compounds | Year: 2015

A new polycyclic aromatic hydrocarbon (PAH)-degrading Gram-negative bacterium Aeromonas salmonicida subsp. achromogenes strain NY4 was isolated from coking wastewater sludge. Strain NY4 could not grow on1-hydroxy-2-naphthoic acid, hexadecane or hexanol as sole carbon source but phenanthrene. This strain was able to utilize several known microbial metabolites of PAHs, including catechol, protocatechuic acid, salicylic acid, and phenol as sole carbon source. The mixture of the acidic and neutral fraction, acidic fraction alone and neutral fraction alone of phenanthrene metabolites were prepared and analyzed by GC-MS. A total of at least 27 metabolites were identified as the degradation products of phenanthrene by strain NY4. Among them, 1-hydroxy-α-oxo-2-naphthaleneacetic acid, 2-hydroxy-α-oxo-1-naphthaleneacetic acid, 2-hydroxy-1-(1-hydroxy-2-naphthalenyl)-ethanone, 1,2-dihydro naphtha [2,1-b] furan-2-ol and 2,3-dihydro naphtha [1,2-b] furan-2-ol have not been reported as the metabolites of phenanthrenes so far. The accumulation patterns of phenanthrene metabolite accumulation by strain NY4 depended on the incubation time, inoculum size and concentration of phenanthrene. Based on the degradation metabolites of phenanthrene by strain NY4, degradation pathways are proposed. This is the first report of PAH degradation in Aeromonas salmonicida, which lay the foundation for recognition and further characterization of PAH degradation metabolites and pathways in Aeromonas salmonicida strains. 2015 Copyright © Taylor & Francis Group, LLC


PubMed | Shaanxi Province Academy of science and Institute of Botany of Shaanxi Province
Type: Journal Article | Journal: Acta crystallographica. Section E, Crystallographic communications | Year: 2015

The title compound, C15H22O3H2O, is a natural producr isolated from Chloranthus japonicus, which is a eudesmane sesquiterpenoid. The two trans-fused six-membered rings have chair confomations. In the crystal, O-HO hydrogen bonds link the components into corrugated layers parallel to the bc plane. There are C-HO inter-actions present within and between the layers.

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