Shaanxi Key Laboratory of Molecular Biology for Agriculture

Yangling, China

Shaanxi Key Laboratory of Molecular Biology for Agriculture

Yangling, China
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Zhou J.-H.,Northwest University, China | Li K.,Northwest University, China | He B.-R.,Northwest University, China | Hu Y.-G.,Northwest University, China | And 2 more authors.
Acta Agronomica Sinica | Year: 2010

The sterile gene of YM-type thermo-sensitive male sterile wheat (Triticum aestivum L.) line has been mapped on chromosome arm 1BS, which was more than 10 cM distant from the adjacent molecular markers. For detecting markers with short distances to this gene, a population with 200 F 2 plants was constructed from the cross between ATM3314 and the restorer line Chinese Spring. Among 20 simple sequence repeat (SSR) markers distributing evenly on 1BS, 5 SSRs showed polymorphism between the parents and between the male sterile and fertile bulks. A 1BS partial linkage map carrying the 5 markers was obtained and QTLs for the male sterility were identified using composite interval mapping method. One major QTL and one minor QTL were detected and designated rfv1-1 and rfv1-2, respectively. QTL rfv1-1 (LOD = 8.80) was located between markers Xgwm18 and Xwmc406 with the genetic distances of 6.0 cM and 4.6 cM to the adjacent markers, respectively. On this locus, the additive and dominant effects were 23.87 and 10.44, respectively, which accounted for 23.9% of the phenotypic variation. QTL rfv1-2 (LOD = 3.10) was mapped between markers Xwmc406 and Xbarc8 with the genetic distances of 4.0 cM and 3.4 cM, respectively. This locus had additive effect of 17.59 and dominant effect of 5.99, and explained 7.8% of the phenotypic variation. These results are propitious for fine mapping and positional cloning of this male sterile gene. © 2010 Crop Science Society of China and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences.

Wang H.,Northwest University, China | Lu Y.,Northwest University, China | Chen J.,Northwest University, China | Li J.,Northwest University, China | And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

The extraction and determination of cytisine, sophocarpine, matrine, sophoridine and oxymatrine in Sophora flavescens Ait. were performed using subcritical water extraction and capillary electrophoresis with field-amplified sample stacking. The effects of extraction temperature, pressure, time and cycle number on the extraction yields were investigated systematically for accelerated solvent extraction with ethanol (ASE) and accelerated solvent extraction with water (subcritical water extraction, SWE). The extraction yields obtained using SWE, ASE, water ultrasonic extraction and chloroform soaking extraction methods were compared. The electrophoresis separation buffer was monosodium phosphate (pH 3.0; 110mM)-isopropanol (85:15, v/v). The effect of phosphoric acid added to the sample matrix on the reproducibility of the peak heights of the analytes was also examined. Cytisine, sophoridine and oxymatrine showed good linearity (R 2>0.999) within 0.125-4.0μg/mL, and sophocarpine and matrine exhibited good linearity (R 2>0.998) within 0.0625-2.0μg/mL, with the detection limits in the range of 0.004-0.0013μg/mL. The five alkaloid contents in medicinal plants from different sources and Sophora instant granule were determined and compared. © 2011 Elsevier B.V.

Liu W.,Northwest University, China | Li L.,Northwest University, China | Wang J.-C.,Northwest University, China | Tu Q.,Northwest University, China | And 4 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2012

Microfluidic trapping methods create significant opportunities to establish highly controlled cell positioning and arrangement for the microscale study of numerous cellular physiological and pathological activities. However, a simple, straightforward, dynamic, and high-throughput method for cell trapping is not yet well established. In the present paper, we report a direct active trapping method using an integrated microfluidic device with pneumatic microstructures (PμSs) for both operationally and quantitatively dynamic localization of cells, as well as for high-throughput cell patterning. We designed and fabricated U-shape PμS arrays to replace the conventional fixed microstructures for reversible trapping. Multidimensional dynamics and spatial consistency of the PμSs were optically characterized and quantitatively demonstrated. Furthermore, we performed a systematic trapping investigation of the PμSs actuated at a pressure range of 0 psi to 20 psi using three types of popularly applied mammalian cells, namely, human lung adenocarcinoma A549 cells, human hepatocellular liver carcinoma HepG2 cells, and human breast adenocarcinoma MCF-7 cells. The cells were quantitatively trapped and controlled by the U-shape PμSs in a programmatic and parallel manner, and could be opportunely released. The trapped cells with high viability were hydrodynamically protected by the real-time actuation of specifically designed umbrella-like PμSs. We demonstrate that PμSs can be applied as an active microfluidic component for large-scale cell patterning and manipulation, which could be useful in many cell-based tissue organization, immunosensor, and high-throughput imaging and screening. © 2012 The Royal Society of Chemistry.

Zhang J.,Northwest University, China | Zhang J.,Shaanxi Key Laboratory of Molecular Biology for Agriculture | Liu F.,Northwest University, China | Liu F.,Shaanxi Key Laboratory of Molecular Biology for Agriculture | And 4 more authors.
PLoS ONE | Year: 2015

Nutritional and medicinal benefits have been attributed to the consumption of tissues from the black-boned chickens in oriental countries. Lueyang black-boned chicken is one of the native chicken breeds. However, some birds may instead have white or lighter skin, which directly causes economic losses every year. Previous studies of pigmentation have focused on a number of genes that may play important roles in coat color regulation. Illumina2000 sequencing technology was used to catalog the global gene expression profiles in the skin of the Lueyang chicken with white versus black skin. A total of 18,608 unigenes were assembled from the reads obtained from the skin of the white and black chickens. A total of 649 known genes were differentially expressed in the black versus white chickens, with 314 genes that were up regulated and 335 genes that were down-regulated, and a total of 162 novel genes were differentially expressed in the black versus white chickens, consisting of 73 genes that were up-regulated (including 4 highly expressed genes that were expressed exclusively in the skin of the black chickens) and 89 genes that were down-regulated. There were also a total of 8 known coat-color genes expressed in previous studies (ASIP, TYR, KIT, TYRP1, OCA2, KITLG, MITF and MC1R). In this study, 4 of which showed greater expression in the black chickens, and several were up-regulated, such as KIT, ASIP, TYR and OCA2. To our surprise, KITLG, MITF and MC1R showed no significant difference in expression between the black- and white-skinned chickens, and the expression of TYRP1 was not detected in either skin color. The expression of ASIP, TYR, KIT, TYRP1, OCA2, KITLG, MITF and MC1R was validated by real-time quantitative polymerase chain reaction (qPCR), and the results of the qPCR were consistent with the RNA-seq. This study provides several candidate genes that may be associated with the development of black versus white skin. More importantly, the fact that the MC1R gene showed no significant difference in expression between the black and white chickens is of particular interest for future studies that aim to elucidate its functional role in the regulation of skin color. Copyright: © 2015 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Liu W.,Northwest University, China | Li L.,Northwest University, China | Wang X.,Northwest University, China | Ren L.,Northwest University, China | And 4 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2010

We presented an integrated microfluidic system for dynamical study of cell-microenvironmental interactions. We demonstrated its precisely spatio-temporal control in the flow direction and the multi-site staying of the fluids by groups of monolithic microfabricated valves through digital operation, aside from the regulated communication between two loci based on real-time microenvironment transition. Using this system, a series of functional manipulations, including specific delivery, addressable surface treatment, positional cell loading and co-culture were performed quickly and efficiently for biological applications. Sequentially, we carried out the potential utility of this system in the research of dynamic microenvironmental influence to cells using a patho-physiological interaction during cancer initiation and progression. Our results exhibit the passive role but collaborative response of NIH 3T3 fibroblasts to the soluble signals from hepatocellular carcinoma cells, and also the variable behaviors of carcinoma cells under different environmental stimulation. This system can facilitate the in vitro investigation of cell-microenvironmental interactions occurred in numerous biological and pathogenic processes. © 2010 The Royal Society of Chemistry.

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