ZHANG C.,Northwest University, China |
ZHANG C.,Shaanxi Key Laboratory of Degradable Biomedical Materials |
FAN D.,Shaanxi Key Laboratory of Degradable Biomedical Materials |
FAN D.,Northwest University, China |
And 9 more authors.
Chinese Journal of Chemical Engineering | Year: 2010
In order to improve the production of human-like collagen III (HLC III) by fed-batch culture of recombinant Escherichia coli BL21, the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters. Three variables (induction time, inoculum age and pH), which have significant effects on HLC III production, were selected from eight variables by Plackett-Burman design. With the regression coefficient analysis in the Box-Behnken design, a relationship between HLC III production and three significant factors was obtained, and the optimum levels of the three variables were as follows: induction time 3.2h, inoculum age 12.6 h and pH 6.7. The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant. An average 9.68 g·L-1HLC III production was attained in the validation experiment under optimized condition, which was 80% higher than the yield of 5.36 g·L-1 before optimization. © 2010 Chemical Industry and Engineering Society of China (CIESC) and Chemical Industry Press (CIP).
Yu Y.Y.,Shaanxi Normal University |
Yu Y.Y.,Shaanxi Key laboratory of Degradable Biomedical Materials
Advanced Materials Research | Year: 2013
This work describes the aqueous-solution technique preparation and characterization of the complex between human-like collagen and copper (II). Pure human-like collagen and the copper complex were characterized by thermo gravimetric (TG) and differential scanning calorimetry (DSC) analysis. The TG curves and DSC data reveal the significant thermal differences between pure protein and the copper complex, demonstrating the formation of a new chemical compound, which is different from the free HLC and with more stable thermodynamic properties. © (2013) Trans Tech Publications, Switzerland.