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Wang S.,No 1 Hospital Of Xian | Wang S.,Shaanxi Institute of Ophthalmology | Tian Y.,No 1 Hospital Of Xian | Zhu H.,No 1 Hospital Of Xian | And 7 more authors.
Cornea | Year: 2015

Purpose: To describe a surgical technique for repair of an intractable sclerocorneal melt caused by a serious chemical burn. Methods: This study includes a technique description and review of 3 representative cases. Results: The combination of tenonplasty with a free oral buccal mucosa autograft was used in 3 patients with sclerocorneal melts caused by chemical burns. Promising results were found in each of them. The area of the sclerocorneal melt healed successfully after surgery, and the integrities of the eyeballs were salvaged. Conclusions: This technique provides a new method for surgical repair of an intractable sclerocorneal melt caused by a chemical burn. © 2015 Wolters Kluwer Health, Inc. All rights reserved.


Xiao X.,Shaanxi Institute of Ophthalmology | Xiao X.,Shaanxi Key Laboratory of Eye | Xiao X.,XiAn First Hospital | Pan S.,Shaanxi Institute of Ophthalmology | And 11 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2014

The experiments were designed to evaluate the biocompatibility of a plastically compressed collagen scaffold (PCCS). The ultrastructure of the PCCS was observed via scanning electron microscopy. Twenty New Zealand white rabbits were randomly divided into experimental and control groups that received corneal pocket transplantation with PCCS and an amniotic membrane, respectively. And the contralateral eye of the implanted rabbit served as the normal group. On the 1st, 7th, 14th, 21st, 30th, 60th, 90th, and 120th postoperative day, the eyes were observed via a slit lamp. On the 120th postoperative day, the rabbit eyes were enucleated to examine the tissue compatibility of the implanted stroma. The PCCS was white and translucent. The scanning electron microscopy results showed that fibers within the PCCS were densely packed and evenly arranged. No edema, inflammation, or neovascularization was observed on ocular surface under a slit lamp and few lymphocytes were observed in the stroma of rabbit cornea after histological study. In conclusion, the PCCS has extremely high biocompatibility and is a promising corneal scaffold for an artificial cornea. © 2013 Society of Plastics Engineers.


PubMed | Tsinghua University, Shaanxi Institute of Ophthalmology and Xian First Hospital
Type: Journal Article | Journal: International journal of ophthalmology | Year: 2016

To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea.A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy (TEM) and hematoxylin and eosin (H&E) staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype.The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process. In vitro, the methyl thiazolyl tetrazolium results revealed that extracts of the acellular ostrich corneas (AOCs) had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes. The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea (APC) transplantation. The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer.We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft.


Liu X.-N.,Shaanxi Institute of Ophthalmology | Zhu X.-P.,Shaanxi Institute of Ophthalmology | Wu J.,Shaanxi Institute of Ophthalmology | Wang L.-F.,Shaanxi Institute for Food and Drug Control | Yin Y.,Shaanxi Institute of Ophthalmology
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material. Objective: To determine the potential toxic effects of ostrich corneal stromal scaffold on cells. Methods: Cell culture methods were used to culture L-929 cells in the extracts of ostrich acellular corneal stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cells after cultured for 1, 2 and 3 days. Results and Conclusion: After the cells were cultured in the extracts of ostrich acellular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cells was graded as level 1. The cytotoxicity test was conducted according to the "National Standard of the People's Republic of China GB/T16886.5-2003". After cultured in the extracts of ostrich acellular corneal stroma, a small number of cells were round in shape and loosely adherent without intracytoplasmic granules, and cell lysis could be observed occasionally. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that the ostrich acellular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.


Mi S.,University of Reading | Mi S.,Shaanxi Institute of Ophthalmology | David A.L.,University College London | Chowdhury B.,University College London | And 4 more authors.
Tissue Engineering - Part A | Year: 2012

The aim of this study was to construct an artificial fetal membrane (FM) by combination of human amniotic epithelial stem cells (hAESCs) and a mechanically enhanced collagen scaffold containing encapsulated human amniotic stromal fibroblasts (hASFs). Such a tissue-engineered FM may have the potential to plug structural defects in the amniotic sac after antenatal interventions, or to prevent preterm premature rupture of the FM. The hAESCs and hASFs were isolated from human fetal amniotic membrane (AM). Magnetic cell sorting was used to enrich the hAESCs by positive ATP-binding cassette G2 selection. We investigated the use of a laminin/fibronectin (1:1)-coated compressed collagen gel as a novel scaffold to support the growth of hAESCs. A type I collagen gel was dehydrated to form a material mimicking the mechanical properties and ultra-structure of human AM. hAESCs successfully adhered to and formed a monolayer upon the biomimetic collagen scaffold. The resulting artificial membrane shared a high degree of similarity in cell morphology, protein expression profiles, and structure to normal fetal AM. This study provides the first line of evidence that a compacted collagen gel containing hASFs could adequately support hAESCs adhesion and differentiation to a degree that is comparable to the normal human fetal AM in terms of structure and maintenance of cell phenotype. © 2012 Mary Ann Liebert, Inc.


Mi S.,University of Reading | Khutoryanskiy V.V.,University of Reading | Jones R.R.,University of Reading | Zhu X.,Shaanxi Institute of Ophthalmology | And 2 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2011

The experiments were designed to use photochemically cross-linked plastically compressed collagen (PCPCC) gel to support corneal epithelial cells. A plastically compressed collagen (PCC) scaffold was photo cross-linked by UVA in the presence of riboflavin to form a biomaterial with optimal mechanical properties. The breaking force, rheology, surgical suture strength, transparency, ultrastructure, and cell-based biocompatibility were compared between PCPCC and PCC gels. The breaking force increased proportionally with an increased concentration of riboflavin. The stress required to reach breaking point of the PCPCC scaffolds was over two times higher compared to the stress necessary to break PCC scaffolds in the presence of 0.1% riboflavin. Rheology results indicated that the structural properties of PCC remain unaltered after UVA cross-linking. The PCC gels were more easily broken than PCPCC gels when sutured on to bovine corneas. The optical density values of PCPCC and PCC showed no significant differences (p > 0.05). SEM analyses showed that the collagen fibres within the PCPCC gels were similar in morphology to PCC gels. No difference in cell-based biocompatibility was seen between the PCPCC and PCC scaffolds in terms of their ability to support the ex vivo expansion of corneal epithelial cells or their subsequent differentiation evidenced by similar levels of cytokeratin 14. In conclusion, PCPCC scaffold is an optimal biomaterial for use in therapeutic tissue engineering of the cornea. Copyright © 2011 Wiley Periodicals, Inc.


Ren H.-Y.,Northwest University, China | Xue W.-M.,Northwest University, China | Pan S.-Y.,Shaanxi Institute of Ophthalmology | Lin Y.,Northwest University, China | And 2 more authors.
Huaxue Gongcheng/Chemical Engineering (China) | Year: 2012

Aiming at immune rejection study after corneal transplantation, with cyclosporine A as immunosuppressive drug model, sodium alginate as carrier material, and medium chain triglyceride (MCT) and Tween20 as additives, a kind of controlled release microspheres loaded cyclosporine A was prepared by pulse electostastic droplet generation technology. The process parameters were optimized with orthogonal design. The optimized parameters determined are as follows: sodium alginate mass fraction is 0.8%, volume ratio of water to medium chain triglyceride (MCT) is 1:1.5, Tween20 mass fraction is 6.5% and cyclosporine A dosage is 65 mg. The morphology of CsA-loaded microspheres shows satisfied spherical shape. The mean particle size of drug-loaded microspheres is (36.344 ± 0.103) μm, size distribution span is 2.314, and the encapsulated rate of drug is (86.03±0.65)%. With simulated tears that are conformed with drug-leaking conditions as in vitro release media, the CsA-loaded microspheres show satisfied controlled release properties with the result of 7 d cumulative release rate of 56.5%.


Lu Q.,Northwest University, China | Xue W.-M.,Northwest University, China | Fu Y.-L.,Northwest University, China | Lin Y.,Northwest University, China | And 2 more authors.
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | Year: 2013

CsA-PLA nanoparticles were prepared by modified emulsification-solvent evaporation method. The optimized formula for preparation was screened by Box-Behnken design with the entrapment efficiency as the index. The Transmission Electron Microscope and Zetasizer Nano instruments were utilized to investigate the morphology and surface potential, mean particle size and size distribution of the nanoparticles, respectively. Based on determining the content of CsA with HPLC, in vitro release behavior of CsA loaded nanoparticles were studied. Optimized parameters of CsA-PLA nanoparticles preparation predicted by Quality by Design are 115.0 mg PLA, 22.2 mg CsA, 3.45 mL ethylene chloride and 2.55 mL acetone. The nanoparticles prepared under above conditions show satisfactory monodispersity and sphericity with mean size of (168.7±4.3) nm and zeta potential of (-16.9±0.47) mV. Their mean drug loading is 0.323 mg·g-1, and the experimental value of entrapment efficiency (90.73±0.61)% is very close to the predicted value of 91.05%. Under the leaking tank condition, a kind of sustained release characteristics based on pH-dependent matrix solution was shown and cumulative release ratios of 41.1% and 80.4% in simulated gastric and intestinal media were determined at 7th day, respectively. The potential of using Quality by Design to predict and optimize the preparation parameters of CsA loaded PLA nanoparticles was experimentally proved.


Wang Y.,The No1 Hospital Of Xian | Wang Y.,Shaanxi Institute of Ophthalmology | Wei W.,The No1 Hospital Of Xian | Zhang C.,The No1 Hospital Of Xian | And 6 more authors.
Current Eye Research | Year: 2016

ABSTRACT: Purpose: To investigate whether interleukin-1 alpha (IL1A) and interleukin-1 beta (IL1B) polymorphisms are associated with keratoconus (KC) in unrelated Chinese Han patients. Methods: The IL1A (rs2071376) and IL1B (rs1143627, rs16944) polymorphisms were genotyped in 115 unrelated Chinese Han KC patients and 101 healthy Chinese Han volunteers with the Sequenom MassARRAY RS1000. Sequenom Typer 4.0 software, PLINK 1.07, Haploview 4.0 software platform were used to analyze the allelic variants of IL1A and IL1B genes, and their association with KC risk factors were assessed. Results: Among the variants, the three SNPs (rs2071376 in IL1A, rs1143627 and rs16944 in the promoter region of IL1B) were different between the two groups. The A allele of rs2071376 (A > C, p = 0.017, OR = 1.968, 95% C.I. 1.313–3.425), the C allele of rs1143627 (C > T, p < 0.001, OR = 2.864, 95% C.I. 1.631–4.968) and the A allele of rs16944 (A > G, p = 0.002, OR = 2.401, 95% C.I. 1.396–4.161) were associated with a increased risk of KC in Chinese Han patients. This study showed that rs2071376, rs1143627 and rs16944 had significant differences in associations between KC patients and the control group when different genotypes were analyzed in three models (dominant, recessive, and additive). In the haplotype analysis, the two single nucleotide polymorphisms (SNPs), rs1143627 and rs16944 showed strong linkage disequilibrium. In addition, Haplotype “ACA” was found to be associated with a higher risk of developing KC (OR = 12.91, p < 0.001). Conclusion: Keratocyte apoptosis is an initiating event in the pathogenesis of KC which could be induced by the altered levels of IL1 gene. These findings confirmed that polymorphisms in IL1 genes were associated with risk of KC in the Chinese Han population, which help us to gain insight into the pathogenesis of KC. © 2016 Taylor & Francis Group, LLC.


Pan S.-Y.,The First Hospital of Xian | Pan S.-Y.,Shaanxi Institute of Ophthalmology | Xiao X.-H.,The First Hospital of Xian | Xiao X.-H.,Shaanxi Institute of Ophthalmology | And 6 more authors.
Chinese Journal of Ophthalmology | Year: 2011

Dry eye is a common eye disease, and its incidence rate has been escalating. The increased tear osmolarity is one of the main reasons for complaint, damage and inflammation of dry eye patients. With the breakthrough of testing technology for tear osmolarity, more research and application of tear osmolarity was reported, and papers on tear osmolarity of normal eye and dry eye in different regions were also published. In this article, the progress of the tear osmolarity research, the range of tear osmolarity and its application in diagnosis and therapy of dry eye was introduced, and the prospect for the clinical application of hypotonic artificial tears was also discussed. © 2011 by the Chinese Medical Association.

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