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Liu L.,Fudan University | Liu L.,Shaanxi Coal and Chemical Technology Institute Co. | Yu M.,Fudan University | Zhang Y.,Fudan University | And 2 more authors.
ACS Applied Materials and Interfaces | Year: 2014

In view of the biological significance of glycosylation for human health, profiling of glycoproteome from complex biological samples is highly inclined toward the discovery of disease biomarkers and clinical diagnosis. Nevertheless, because of the existence of glycopeptides at relatively low abundances compared with nonglycosylated peptides and glycan microheterogeneity, glycopeptides need to be highly selectively enriched from complex biological samples for mass spectrometry analysis. Herein, a new type of hydrazide functionalized core-shell magnetic nanocomposite has been synthesized for highly specific enrichment of N-glycopeptides. The nanocomposites with both the magnetic core and the polymer shell hanging high density of hydrazide groups were prepared by first functionalization of the magnetic core with polymethacrylic acid by reflux precipitation polymerization to obtain the Fe3O 4@poly(methacrylic acid) (Fe3O4@PMAA) and then modification of the surface of Fe3O4@PMAA with adipic acid dihydrazide (ADH) to obtain Fe3O4@poly(methacrylic hydrazide) (Fe3O4@PMAH). The abundant hydrazide groups toward highly specific enrichment of glycopeptides and the magnetic core make it suitable for large-scale, high-throughput, and automated sample processing. In addition, the hydrophilic polymer surface can provide low nonspecific adsorption of other peptides. Compared to commercially available hydrazide resin, Fe 3O4@PMAH improved more than 5 times the signal-to-noise ratio of standard glycopeptides. Finally, this nanocomposite was applied in the profiling of N-glycoproteome from the colorectal cancer patient serum. In total, 175 unique glycopeptides and 181 glycosylation sites corresponding to 63 unique glycoproteins were identified in three repeated experiments, with the specificities of the enriched glycopeptides and corresponding glycoproteins of 69.6% and 80.9%, respectively. Because of all these attractive features, we believe that this novel hydrazide functionalized core-shell magnetic nanocomposite will shed new light on the profiling of N-glycoproteome from complex biological samples in high throughput. © 2014 American Chemical Society. Source


Wang Y.,Xian University of Science and Technology | Wang Y.,Shaanxi Coal and Chemical Technology Institute Co. | Xia Y.,Xian University of Science and Technology | Du R.,Xian University of Science and Technology
Caikuang yu Anquan Gongcheng Xuebao/Journal of Mining and Safety Engineering | Year: 2014

How to exploit coal resources under the premise of protecting the ecological diving, namely, the implementation of "coal mining under water-containing condition", is a urgent technical problem to be solved in the construction of energy and chemical bases in Northern Shaanxi. Based on numerical simulation, taking a mine field in Northern Shaanxi as the research object, the growth regulation of water flowing fractured zone and the influence on ecological diving under different mining height were studied in this paper. The results show that the development height of water flowing fractured zone is mainly affected by the mining height. In order to realize the goal of "coal mining under water-containing condition", slice and limit-height mining technology should be used in the study area. Based on this, the whole field is reasonably devided by limit mining height, which provides reference to ascertain the maximum mining height of slice mining. Source


Wang L.,Tohoku University | Wang L.,Shaanxi Coal and Chemical Technology Institute Co. | Chen J.,Tohoku University | Watanabe H.,University of Tsukuba | And 4 more authors.
Applied Catalysis B: Environmental | Year: 2014

The Co-Fe/Mg/Al catalysts were prepared from the hydrotalcite-like compounds containing Co, Fe, Mg and Al, and applied to the steam gasification of biomass-derived tar. The characterization using TPR, TPO, XRD, H2 adsorption, TEM and EXAFS indicates that the Co-Fe/Mg/Al catalysts have a nanocomposite structure of the Co-Fe bcc alloy particles and the oxide particles of MgAl2O4-based solid solution after the reduction, where Co metal atoms can substitute partially Fe metal sites in bcc Fe metal regarding the Co-Fe bcc alloy particles. The composition of the Co-Fe/Mg/Al catalysts was optimized on the basis of the activity tests. The optimized Co-Fe/Mg/Al catalyst exhibited higher catalytic performance than the reported catalysts such as Co-Fe/α-Al2O3, Co/Mg/Al, Ni-Fe/Mg/Al in terms of activity and resistance to coke deposition. This behavior can be explained by the formation of the Co-Fe bcc alloy nanoparticles with rather uniform composition on MgAl2O4-based solid solution. © 2014 Elsevier B.V. Source


Lei R.,Shaanxi Coal and Chemical Technology Institute Co. | Gao L.-X.,CAS Changchun Institute of Applied Chemistry | Jin R.-Z.,CAS Changchun Institute of Applied Chemistry | Qiu X.-P.,CAS Changchun Institute of Applied Chemistry
Chinese Journal of Polymer Science (English Edition) | Year: 2014

A series of sulfonated polyimide copolymers as novel proton exchange materials were synthesized by the polycondensation of 1,4,5,8-naphthalene- tetracarboxylic dianhydride (NTDA), sulfonated diamine based on pyridine group and diamine containing N-phenyl-1,2,4-triazole moiety. Flexible, transparent and tough membranes with high thermal stability and good mechanical properties were obtained. They exhibited good stability in boiling water and Fenton's reagent at 80 °C. More interestingly, a nonlinear relationship between proton conductivities of the resulting membranes and the degree of sulfonation (DS) was observed. The membrane with 50% DS exhibited the maximum proton conductivity, which was due to the combinational contributions of sulfonic acid and N-pheny-1,2,4-triazole groups. Thus, the N-phenyl-1,2,4-triazole moiety in this study not only can depress water absorption but also increase proton conductivity, especially at low DS. © 2014 Chinese Chemical Society, Institute of Chemistry, Chinese Academy of Sciences and Springer-Verlag Berlin Heidelberg. Source


Chang J.,CAS Institute of Geology and Geophysics | Chang J.,University of Chinese Academy of Sciences | Li W.,China University of Mining and Technology | Li T.,Shaanxi Coal and Chemical Technology Institute Co. | And 2 more authors.
Caikuang yu Anquan Gongcheng Xuebao/Journal of Mining and Safety Engineering | Year: 2014

Large-scale mining has produced great influence on local fragile eco-environment in Northern Shaanxi, such as leakage of aquifer and reduction of flows. Under this background, to acquire the migration situations of water resources in arid mining area, taking Shennan Mining Area in Northern Shaanxi as example, a series of works were carried out to study the division evaluation of water resources' leakage degree, water resources quantity forecasting after mining, and analysis and comparison of water quality in goaf. The results show that the gross quantity of water resources in the mining area increases after mining, and the water in goaf is the main source of the growth. Consequently, the gross amount of water resources is proposed as the evaluation standard of "water preserved mining". If the gross amount of water resources before mining is greater than that after mining, the water resources are running off on the whole. On the contrary, it is considered that the mining process can improve the occurrence condition of water resources in some specified region, that is, the coal mining is beneficial to water resources protection. From above standard, water resources environment in Shennan Mining Area is improved. Source

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