Xie X.-J.,Shaanxi Center for Clinical Laboratory |
Xie X.-J.,Xi'an Jiaotong University |
Pan J.-J.,Xi'an Jiaotong University |
Wei L.-Q.,Shaanxi Center for Clinical Laboratory |
Chen W.,Xi'an Jiaotong University
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2016
Objective: To provide theoretical guidance for further research on the role of miR-199a-3p in formation and development of bladder cancer. Methods: Mature sequence of miR-199a-3p was analyzed; target genes and transcription factors of miRNA-199a-3p were predicted, and the target genes were analyzed for gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway. Then TF-miRNA- mRNA network diagram was constructed. Results: Sequences of miR-199a-3p were highly conserved in various species. In GO analysis, the target genes of miR-199a-3p were enriched in many biological processes, such as regulation of cellular process, regulation of macromolecule metabolic process, and regulation of biological process (P<0.01). In KEGG pathway, the target genes were mainly located in bacterial invasion pathway of epithelial cells, ECM-receptor interaction pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, small cell lung cancer pathway, and proteoglycans pathway in the cancer (P<0.05). According to the TF-miRNA-mRNA network diagram, the important genes that might be regulated by miR-199a-3p were MYC, SP1, mTOR, NFκB, and NFκB1. Conclusion: miR-199a-3p may directly target mTOR and participate in the formation and development of bladder cancer through regulating PI3K-Akt-mTOR signaling pathway. © 2016, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.