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Du X.,Shaanxi Academy of Traditional Chinese Medicine | Zhu Y.,Liaoning Normal University | Liu S.,Liaoning Normal University | Zhao D.,Liaoning Normal University
Chemical Research in Chinese Universities | Year: 2015

A new approach to calculate the potential acting on an electron in a molecule(PAEM) has been established for drawing the molecular face(MF) of a macromolecule, according to the classic point charge model and the atom-bond electronegativity equalization method(ABEEMσπ) for one electron in a molecule. We introduced a dynamic charge distribution from the view of a local electron movement in a molecule based on the new approach, and as further direct evidence, we calculated some physical quantities using the original ab initio method and the new method to verify the accuracy of the method, such as the boundary distance(BD), molecular face surface area(MFSA) and molecular reactivities indicated by the MFs for a variety of organic molecules. All the results by the new method are in agreement with the results by ab initio method. © 2015, Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH.

Sun T.-T.,Chinese Academy of Sciences | Sun T.-T.,Shaanxi Academy of Traditional Chinese Medicine | Li R.-R.,Chinese Academy of Sciences | Zhang Z.-J.,Chinese Academy of Sciences | And 2 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2015

Reversed phase high-performance liquid chromatography (RP-HPLC) was used to determine the chromatographic fingerprints of multiple batches of decoction pieces of Corydalis yanhusuo samples. The samples were acquired from different production sites, had different numbers of growth years, and were processed using different methods. The standard operating procedure (SOP) for a similarity evaluation system for chromatographic fingerprints of traditional Chinese medicine (version 2004) was used to analyze and evaluate the chromatographic fingerprints. This paper explored the factors that affected the quality of the decoction pieces of Corydalis yanhusuo by analyzing the differences among the chromatographic fingerprints. The results revealed that the processing method is the most important factor contributing to the difference in the chromatographic fingerprints of the samples. This study provides a point of reference for the quality control of decoction pieces of Corydalis yanhusuo samples. Copyright © Taylor & Francis Group, LLC.

Zhang H.,Northwest University, China | Zhang H.,Shaanxi Academy of Traditional Chinese Medicine | Wang H.-F.,Engineering University of | Liu Y.,Northwest University, China | And 3 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance The rhizome of Panax japonicus C. A. Mey. var. major (Burk.) C. Y. Wu et K. M. Feng (PJ) is a commonly used traditional Chinese medicine to promote hematopoietic effects, promote blood circulation and supporting healthy energy. Aim of the study is to investigate the haematopoietic effects of PJ and determine the mechanism of its haematopoietic activity. Materials and methods The crude extract from PJ (PJE) was separated into two fractions: polysaccharides (PJPS) and low-molecular-weight compounds (PJSM). PJPS, and PJSM were incubated with mice spleen cells, and their haematopoietic activities were evaluated by determining the haematopoietic growth factor levels (HGFs) in vitro. The in vivo experiments used anaemia model mice that were given hypodermic injections of N-acetyl phenylhydrazine (APH) and intraperitoneal injections of cyclophosphamide (CTX). Results Both PJPS and PJSM were significantly involved in the haematopoietic effect of PJE. The administration of PJPS and PJSM could accelerate the recovery of the white blood cell (WBC), red blood cell (RBC), and haemoglobin (HGB) levels in the blood deficiency model mice. Haematopoietic activity may result from stimulating the secretion of interleukin-3 (IL-3), interleukin-6 (IL-6), erythropoietin (EPO), GM colony-stimulating factor (CSF), and M-CSF and by the resistance of spleen cells to apoptosis. Conclusions The study results support the potential use of PJPS and PJSM for the treatment of anaemia. © 2014 Elsevier Ireland Ltd.

Liu R.,Shaanxi Normal University | Li J.,Shaanxi Normal University | Cheng Y.,Shaanxi Normal University | Huo T.,Shaanxi Normal University | And 4 more authors.
Food and Function | Year: 2015

The study investigated the effect of pomegranates ellagic acid (PEA) on blood cholesterol and investigated its effects on LXR/RXR/PPAR-ABCA1 nuclear receptors-signaling pathways of cholesterol metabolism on molecular level in hamsters. In this experiment, hamsters were randomly divided into two groups: the first group (NG, n = 9) was always fed the normal diet, whereas the other group (HFG, n = 45) was fed a high fat diet during the first 4 weeks and then fed the normal diet for the last 4 weeks. In HFG, which was divided into five groups (n = 9) during the last 4 weeks, three groups were treated with PEA at 44 mg per kg bw, 88 mg per kg bw and 177 mg per kg bw, one group was treated with simvastatin at 1.77 mg per kg bw, and one was given sterile double-distilled water. The data validated that PEA dose-dependently decreased plasma total cholesterol and triglyceride level accompanied by a greater excretion of fecal bile acid. The result of RT-PCR revealed that PEA up-regulated liver X receptor (LXRα), peroxisome proliferator-activated receptor α (PPARα), peroxisome proliferator-activated receptor γ (PPARγ) and their downstream gene ATP-binding cassette transporter A1 (ABCA1), with no effect on retinoid X receptor (RXRα). PEA promoted cholesterol removal by enhancing fecal bile acid and up-regulation of the two pathways, LXR/PPAR-ABCA1. Moreover, PEA was stronger than simvastatin in some aspects. This journal is © The Royal Society of Chemistry 2015.

Shao A.,Guangdong Pharmaceutical University | Chen G.,Guangdong Pharmaceutical University | Jiang N.,Shaanxi Academy of Traditional Chinese Medicine | Li Y.,Shaanxi Academy of Traditional Chinese Medicine | And 4 more authors.
Archives of Pharmacal Research | Year: 2013

The aim of this study was to develop and characterize a self-microemulsifying drug delivery system (SMEDDS) of Brucea javanica oil (BJO) and transform the liquid formulation into solid granules. Solubility studies of BJO and pseudo-ternary phase diagrams were used to identify the most efficient self-emulsification region. A methyl thiazolyl tetrazolium (MTT) assay was performed to identify cell apoptosis. Antitumor activity studies were also employed to evaluate the BJO SMEDDS. The optimized BJO SMEDDS in liquid and granule formulations rapidly formed fine oil-in-water microemulsions with particle sizes <50 nm. Additionally, the MTT assay demonstrated that BJO SMEDDS had a significant effect on cancer cells, and antitumor activity studies showed remarkable inhibition of S180 tumors. The BJO SMEDDS, optimized to have good characteristics, was successfully transformed into solid granules by adsorbing onto crospovidone. The studies of the release of the BJO SMEDDS of liquid and granules in vitro suggested that the release of BJO was enhanced by the SMEDDS. These studies revealed that the new self-microemulsifying systems of liquid and granule forms might be promising strategies for the oral delivery of the poorly water-soluble drug BJO. © 2013 The Pharmaceutical Society of Korea.

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