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Chen H.,Shaanxi University of Science and Technology | Li C.,Shaanxi University of Science and Technology | Shu G.,Shaanxi Academy of science | Wang C.,Shaanxi University of Science and Technology
Advanced Materials Research | Year: 2012

This study demonstrates that the type of nitrogen sources has an important influence on the growth of Streptococcus thermophilus.at the same time, viable counts was studied by in the medium containing various nitrogen sources (peptone, yeast extract, meat extract, tryptone, soya peptone and casein hydrolysate). The results indicated that soya peptone was the most efficient nitrogen source and and the influence of different concentrations of soya peptone on growth was determined. The results indicated that viable bacteria were stimulated by the high soya peptone concentration (30g/L). © (2012) Trans Tech Publications, Switzerland. Source


Yang F.,PLA Fourth Military Medical University | Wan Y.,Shaanxi Academy of science | Liu J.,PLA Fourth Military Medical University | Yang X.,PLA Fourth Military Medical University | And 5 more authors.
Journal of Microbiological Methods | Year: 2016

Various protocols for recombinant Interleukin-10 (IL-10) purification in wound healing have been reported previously. However, the therapeutic effect was not obvious. Thus, it is of great importance to find new and effective approaches for therapy. In this study, we propose that IL-10 and Arginine-Glycine-Aspartic (RGD) peptide would be a valuable therapeutic for wound healing. To explore a high-efficiency and cost-effective approach for the production of IL-10 and RGD peptide with bioactivity, a synthetic gene was cloned into a recombinant pTWIN1 vector. As a consequence, rhIL-10-RGD and the pH-induced self-cleavable Ssp DnaB mini-intein as a fusion protein was highly expressed by IPTG induction in Escherichia coli Rosetta without extra residues in a bioreactor. After Ni affinity chromatographic purification, rhIL-10-RGD was released by the Ssp DnaB intein-mediated self-cleavage that is triggered by pH shift. SDS-PAGE and silver staining showed a major band with an estimated molecular mass of 19.3 kDa. Cell proliferation assay confirmed its potent proliferation activity on MC/9 murine mast cells. In conclusion, we report a novel strategy to produce rhIL-10-RGD mediated by the pH-induced self-cleavable Ssp DnaB mini-intein, and show that rhIL-10-RGD could play an effective role in wound healing of BALB/c mice. © 2016 Elsevier B.V. Source


Shu G.,Shaanxi University of Science and Technology | Shu G.,Shaanxi Academy of science | Yang H.,Shaanxi University of Science and Technology | Tao Q.,Shaanxi Academy of science | He C.,Shaanxi University of Science and Technology
Advance Journal of Food Science and Technology | Year: 2013

The effects of ascorbic acid and L-Cysteine Hydrochloride (Cys-HCl) on growth of Bifidobacterium bifidum BB01 and BB03 were studied by using MRS broth as the control, the concentrations of ascorbic acid and Cys-HCl were both of 0.4, 0.6, 0.8, 1.0 and 1.2g/L. The result showed as follows: ascorbic acid 0.8g/L or Cys-HCl 0.6g/L on growth of B. bifidum BB01 and ascorbic acid 0.4g/L and Cys-HCl 0.4g/L on growth of B. bifidum BB03 had significant influence (p<0.05), respectively. The OD600 of B. bifidum BB01 and B. bifidum BB03 reached 1.32, 1.32, 1.347 and 1.296 at optimal concentration of ascorbic acid and Cys-HCl mentioned above, while the control only reached 1.121 and 1.213 respectively. © Maxwell Scientific Organization, 2013. Source


Xu S.-Y.,Shaanxi Academy of science | Xu A.-G.,Shaanxi Textile Science Institute | Zhao W.-J.,Shaanxi Academy of science | Ma Q.,Shaanxi Engineering Center for Enzyme Technology | Ren P.,Shaanxi Engineering Center for Enzyme Technology
Wool Textile Journal | Year: 2012

In this paper, we researched the technology of the shrink-resist finishing of wool fabrics with the immobilized neutral proteinase, prcessed the single-actor experiment. The application technological parameter of the immobilized neutral proteinase was optimized by the orthogonal experimental design. The result showed that the amount of the immobilized neutral proteinase was 5% ( owf ) , the treating temperature was 50 °C , the treating pH value was 7. 0 and the treating time was 60 min. The results finishing the wool fabrics with the free and immobilized neutral proteinase were compared. The result showed that the shrinkage effect was increased 37. 6% , the weight loss percentage was descended 45. 2% , the strength loss percentage was descended 52. 7% , the whiteness was increased 23. 2% and the anti-pill rate was raised by level 2. This will establish the basis of the industrializing enzvme on shrink-resist finishing of wool knitted fabrics. Source


Chen H.,Shaanxi University of Science and Technology | Ma Z.,Shaanxi University of Science and Technology | Shu G.,Shaanxi University of Science and Technology | Shu G.,Shaanxi Academy of science | And 2 more authors.
Advanced Materials Research | Year: 2012

In the present study, the experiments were investigated with effecting of sucrose, lactose, glucose, maltose on survival of Lactobacillus acidophilus during freeze-drying. In order to reach sufficient numbers viable cells, so different concentrations of protective agents were designed in the experiment, which as 3%, 6%, 9%, 12% and 15%, respectively. Measuring the survival ratio of Lactobacillus acidophilus and number of viable cells after completed drying. There were significant differences in survival during freeze-drying. Results showed that the protective agent was a critical factor affecting the survival of Lactobacillus acidophilus. In the experiments, the highest survival rate was soluble starch as the protective agent, and when the protective agent as galactose the number of viable cells was largest. Source

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