Entity

Time filter

Source Type


Oya M.,University of Tokyo | Kitaguchi T.,SGP Group | Yanagihara Y.,University of Tokyo | Numano R.,Toyohashi University of Technology | And 3 more authors.
Biochemical and Biophysical Research Communications | Year: 2013

Recent studies have suggested that astrocytes release gliotransmitters (i.e., ATP, L-glutamate, D-serine, and peptide hormones) and participate actively in synaptic functioning. Although ATP release from astrocytes modulates the activity of neurons, the mechanisms regulating the ATP release from astrocytes and the source of ATP in astrocytes are not well understood. Recently a vesicular nucleotide transporter (VNUT)/solute carrier family 17, member 9 (SLC17A9) has been identified as a mediator of the active accumulation of ATP into vesicles. Here we show by immunocytochemical analysis under confocal microscope and live cell imaging under total internal reflection fluorescence microscope that lysosome-associated VNUT is responsible for ATP release in astrocytes. VNUT was expressed in both primary cultured cortical astrocytes and glioma cell line C6 cells, and mainly localized on lysosome in the cells. We found that VNUT-associated secretory lysosomes do not fully collapse into the plasma membrane after lysosomal exocytosis. We also found that inhibition of VNUT function by Evans Blue decreased ATP uptake into secretory lysosomes. Depletion and inhibition of endogenous VNUT by small interference RNA and Evans Blue, respectively decreased the amount of ATP release from the cells, whereas overexpression of VNUT increased it. Taken together, these findings indicate that the participation of VNUT in ATP storage in secretory lysosomes during lysosomal exocytosis of ATP from astrocytes. © 2013 Elsevier Inc. Source


SGP Group | Entity website

La Feria de Importacin y Exportacin de China, tambin llamada como la Feria de Cantn, fundada en la primavera de 1957, que tiene lugar cada ao en la primavera y el otoo y ms de 56 aos de existencia, es actualmente un gran encuentro general de comercio internacional de China, que cuenta con una historia ms larga, una envergadura ms grande, variedades ms completas de artculos expuestos, con participantes venidos de pases y regiones ms amplios y mejores resultados de transaccin del Pas. Compuestas por ms de 20 mil mejores corporaciones (empresas) para el comercio exterior, incluyendo compaas exteriores de comercio, fbricas, instituciones de investigacin cientfica, empresas con inversin exterior, empresas con inversin enteramente exterior, empresas privadas, etc ...


SGP Group | Entity website

Nuestro software de administracin de pedidos ayuda a mejorar el servicio al cliente y aumentar los ingresos. Nuestros representantes pueden acceder, en tiempo real, al inventario en toda la compaa, y el sistema automticamente hace referencia a las necesidades de cada del cliente ...


Tienda Inglesa Uruguay es una de esas pocas empresas que en Uruguay pueden contar una historia centenaria, creada en a dcada de 1870 y hoy en da con mas 140 aos Tienda Inglesa, con razn social Henderson & Ca. S ...

Discover hidden collaborations