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Agency: National Science Foundation | Branch: | Program: STTR | Phase: Phase I | Award Amount: 100.00K | Year: 2006

This Small Business Technology Transfer (STTR) Phase I project aims to develop a magnetohydrodynamically (MHD) based, closed-loop liquid chromatographic (LC) technology. The work will improve on exiting technologies to allow more specific purification of desired materials. Existing LCs consist of a fixed length column that cannot adjust according to the separation task in mind. Using a column bent into a closed loop that has virtual infinite length and should allow one to achieve very precise separations and purification of compounds will alleviate the MHD system. Liquid chromatography is a mature technology that is often used in chemical, biological, and medical laboratories for separation and purification of macromolecules. The shortcomings of fixed length columns have long been recognized. This novel MHD approach will allow for increased separation and thus better purification of such macromolecules for use as research reagents as well as in the drug development market.

Arya S.K.,Institute of Microelectronics, Singapore | Pui T.S.,Institute of Microelectronics, Singapore | Wong C.C.,Institute of Microelectronics, Singapore | Kumar S.,Sfc Fluidics, Llc | Rahman A.R.A.,Institute of Microelectronics, Singapore
Langmuir | Year: 2013

In the present work, the effect of a surface modification protocol along with the electrode size has been investigated for developing an efficient, label-free electrochemical biosensing method for diagnosis of traumatic brain injury (TBI) biomarkers. A microdisk electrode array (MDEA) and a macroelectrode with a comb structure (MECS) were modified with an anti-GFAP (GFAP = glial fibrillary acidic protein) antibody using two protocols for optimum and label-free detection of GFAP, a promising acute-phase TBI biomarker. For the MDEA, an array of six microdisks with a 100 μm diameter and, for the MECS, a 3.2 mm × 5.5 mm electrode 5 μm wide with 10 μm spaced comb fingers were modified using an optimized protocol for dithiobis(succinimidyl propionate) (DSP) self-assembled monolayer formation. Anti-GFAP was covalently bound, and the remaining free DSP groups were blocked using ethanolamine (Ea). Sensors were exposed to solutions with different GFAP concentrations, and a label-free electrochemical impedance spectroscopy (EIS) technique was used to determine the concentration. EIS results confirmed that both types of Ea/anti-GFAP/DSP/Au electrodes modified with an optimized DSP-based protocol can accurately detect GFAP in the range of 1 pg mL-1 to 100 ng mL-1 with a detection limit of 1 pg mL-1. However, the cross-use of the MDEA protocol on the MECS and vice versa resulted in very low sensitivity or poor signal resolution, underscoring the importance of proper matching of the electrode size and type and the surface modification protocol. © 2013 American Chemical Society. Source

Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 165.78K | Year: 2011

DESCRIPTION (provided by applicant): Portable brain injury biomarker detection system with integrated microdialysis probe Project summary/Abstract: This SBIR application aims to integrate microdialysis with an effective, miniature pumping system and adetection system for analyzing different biomarkers in real-time and without user intervention. An integrated portable device consisting of SFC Fluidics' proprietary ePump(R), snap-on magnetic latches for rapid fluidic connection, a reagent reservoir and amicrofluidic detection system will be developed. This system will facilitate real time continuous monitoring of pathological biomarkers, which in turn helps in better patient management over the course of healing/therapy. The focus of this SBIR is on monitoring Traumatic Brain Injury (TBI) sequelae with a microdialysis based system using a set of low molar mass biomarkers and a leading large molecule (protein) biomarker. In Phase I, commercially-available ELISA kits will be incorporated into SFC Fluidics integrated microdialysis and fluid handling system. The Phase II focus will be on development of a single prototype system that can quantify multiple TBI biomarkers. Phase I Research Plan Specific Aim: Design and engineer brain injury biomarker detection system with integrated microdialysis probe Task 1: Design and fabricate ePump driven integrated flow system Task 2: Detection of small molecule brain injury biomarkers Task 3: Detection of large molecule brain injury biomarker PUBLIC HEALTH RELEVANCE:An estimated 1.7 million Traumatic Brain injury (TBI) cases are reported every year in United States with 80.7 percent of them resulting in emergency department visits. There is an urgent need for development of a portable, microdialysis system with in-built analyzer which will help in rapid and continuous diagnosis of the state of health of a patient with TBI. Early and timely diagnosis of the pathological condition will help a doctor to take remedial actions and improve the quality of care of the patient while greatly mitigating long-term complications.

Agency: National Science Foundation | Branch: | Program: STTR | Phase: Phase II | Award Amount: 500.00K | Year: 2008

This STTR Phase II research project develops a circular chemical separation system on a small (~1 inch x 1 inch) chip. This chip and the associated instrument will separate complex mixtures for biological, chemical, medical, and industrial applications. Based on magnetohydrodynamic (MHD)-driven liquid flow, liquid chromatographic (LC) separations will be accomplished in a circular, closed-loop format. Typically, LC separations require a sample containing multiple analytes to flow in a single direction along a fixed-length, linear column with detection performed after the analytes elute from the column. In the circular LC system, miniaturization is possible because samples are instead circulated around a closed-loop chromatographic column thus, the effective column length is not limited to small chip dimensions. Very few methods can provide the mobile-phase pumping in a closed-loop that is required for practical application of circular LC. The MHD-based circular LC system envisioned will be small, portable, and designed for laboratory as well as field use. The sealed LC chip will contain the stationary phase, mobile phase, and all in situ MHD pumps needed to conduct the separation of complex samples. This prototype LC instrument will be designed and fabricated with a built-in fluorescence detector for monitoring analyte separation directly on the chromatographic column. The broader impacts of this research are highlighted by the ability of the proposed circular separation system to miniaturize a valuable analytical tool, liquid chromatography (LC). Samples of interest include human blood serum, saliva, and urine, with component analytes of interest that are equally diverse (e.g. proteins, pharmaceuticals, and small molecular biomarkers). Many analytes in these complex mixtures have similar properties and cannot be separated and analyzed using a very short chromatographic column, which has limited the miniaturization of this important analytical tool. This limitation is overcome using circular LC, where the effective column length is not limited by the small chip sizes that are essential for portable LC instrumentation. SFC Fluidics' core technology makes possible the miniaturized, closed-loop pumping required for implementation. This method has broad implications for the portable LC systems for field deployment or point-of-care applications. The market opportunity is expected to be significant, particularly when considering that applicability extends beyond the traditional instrumentation market into the worldwide point-of-care diagnostics market.

Agency: National Science Foundation | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 100.00K | Year: 2008

This Small Business Innovation Research Phase I research proposal targets technical innovations that will advance the implementation of micro-scale bioassays, lab-on-a-chip applications, and electrospray mass spectrometry approaches to proteomics. It is proposed to design and fabricate miniature, stand-alone non-mechanical pumps with a diversity of shapes. Each micropump will have a footprint of less than 1 in2 and be capable of controlled, precise flow rates from nL/min to microL/min. The non-mechanical nature and operating principles that govern the ePump afford an unusual degree of freedom in pump design, extending to include a wide range of pump shapes and sizes. Delivering pulse-free flow, this new type of pump will be compatible with a broad range of assay solvents and solutions. The target is a miniature pump that can be implemented in a range of shapes that can be readily adapted to the constrained spaces within OEM assay systems. Market opportunities for the proposed stand-alone pumps exist in research and commercial chromatography, microfluidics, proteomics, and sample introduction. Additional opportunities are represented for drug delivery and IV therapy. The unique miniature pumps will advance biotechnology on a number of fronts including lab-on-a-chip, micro-total analysis systems, and point-of-care devices. A direct impact on public health will be realized by increased portability and general accessibility of diagnostic and measurement systems.

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