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Dall'Aglio C.,Sezione di Anatomia Veterinaria | Pascucci L.,Sezione di Anatomia Veterinaria | Mercati F.,Sezione di Anatomia Veterinaria | Boiti C.,Laboratorio Of Biotecnologie Fisiologiche | Ceccarelli P.,Sezione di Anatomia Veterinaria
Acta Histochemica | Year: 2012

The aim of the present study was to investigate by immunohistochemistry the presence and distribution of the orexin system in the stomach and gut of fallow deer. Abundant orexin A-positive cells were localized in the middle and basal portions of the mucosal glands of the cardial and fundic regions of the stomach. In the same gastric areas, orexin B-positive cells were also found, mainly localized in the basal portion of glands. In the intestinal tract, orexin-containing cells were occasionally found in the duodenal epithelium and in the rectal intestinal glands. Immunoreactivity for orexin receptors, type 1 and 2 (OX1R and OX2R), was not detected in the same stomach regions. OX1R-immunopositivity was observed in the enteric neuron ganglia localized in the submucosal and muscular intestinal layers, while OX2R-immunopositivity was found close in contact with the cytoplasmic membrane of epithelial cells in the small intestine. © 2011 Elsevier GmbH. Source


Mercati F.,Sezione di Anatomia Veterinaria | Dall'Aglio C.,Sezione di Anatomia Veterinaria | Pascucci L.,Sezione di Anatomia Veterinaria | Boiti C.,Laboratorio Of Biotecnologie | Ceccarelli P.,Sezione di Anatomia Veterinaria
Acta Histochemica | Year: 2012

In veterinary medicine, there is an increasing interest in the study of the endo-cannabinoid system and the possible use of the cannabinoids for the treatment of several diseases. Cannabinoid receptors (CB) are widely distributed in human and laboratory animal tissues, justifying the involvement of the endo-cannabinoid system in a great number of metabolic ways. Since there are no data regarding cannabinoid receptors in hair follicles of domestic animals, we investigated the presence and localization of CB1 receptor in dog hair follicles. By using a goat anti-CB1 polyclonal antibody, we observed CB1 receptor in the proximal part of both primary and secondary hair follicles. Staining was localized in the inner root sheath cells. We suppose that the endo-cannabinoid system is involved in the molecular mechanisms regulating hair follicle activity in dog. The identification of CB1 receptor at the level of the inner root sheath may help in the understanding of hair follicle biology and the possibility that cannabinoid molecules could be considered as suitable therapeutic tools in dog. © 2011 Elsevier GmbH. Source

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