Kumar R.,Bioinformatics Section |
Kumar S.,Sewar |
Sangwan S.,Chaudhary Devi Lal University |
Yadav I.S.,Bioinformatics Section |
Yadav R.,Guru Jambheshwar University of Science and Technology
Journal of Molecular Graphics and Modelling | Year: 2011
Myrosinase, the only known S-glycosidase, occurs particularly in Cruciferae family. It is responsible for the hydrolysis of glucosinolates and serves as a vital element of plant defense system. The biological and chemical properties of myrosinase catalyzed products of glucosinolates are well characterized. The myrosinase-protein-sequence of Brassica juncea was retrieved from NCBI database and its 3-D model was generated on the basis of crystal structure of 1MYR-A, 1E4M-M and 1DWA-M chains of myrosinase from Sinapis alba by employing Modeller9v7 program. Homolog templates from S. alba exhibited 72% identity with target sequence. The model was optimized by using molecular dynamics (MD) approach together with simulated annealing (SA) methods in the same Modeller program, and eventually verified and validated on SAVES (Structure Analysis and Verification Server) and PROCHECK programs, respectively. Ramachandran plot obtained through PROCHECK program depicted that 99.8% of total residues were confined to the allowed region while only one residue (Thr92) was restrained to the disallowed region. Additionally, B. juncea myrosinase contains three disulphide bridges which were found to be conserved in S. alba homologs as well. Further, overlapping of B. juncea myrosinase with that of template protein 1MYR-A from S. alba stipulates the amino acid residues Arg115, Gln207, Thr210, Asn350, Tyr352 and Glu429 that constitute active site of the enzyme. Active site analysis also speculates the presence of a hydrophobic pocket in addition to seven N-glycosylation sites. Docking studies of enzyme and substrate illuminate the interactions of various active site residues with diverse groups of sinigrin. Therefore, the present study furnishes the first significant, in silico insight into the 3-D structure, active site machinery, and enzyme-substrate interactions of B. juncea myrosinase. © 2010 Elsevier Inc.
Singh V.V.,Sewar |
Chauhan J.S.,Sewar |
Meena M.L.,Sewar |
Indian Journal of Agricultural Sciences | Year: 2014
A random mating population of Indian mustard [Brassica juncea (L.) Czern. & Coss.] utilizing 20 germplasm accessions/promising donors for component traits, was developed from 2007-08 to 2009-10. Single plant progenies (180) termed as 'half sibs' were developed from this population and evaluated during 2010-11 in augmented block design along with standard checks. Observations were recorded on siliquae/ plant, siliquae length, seeds/siliqua, seed yield/plant and 1000-seed weight. The estimates of heritability were observed to be high 59.16 to 97.94% for all the characters. High heritability coupled with high genetic advance and GCV were observed for siliquae/plant and seed yield/plant. Maximum number of half sib progenies showed significant superiority for 1000 seed weight (39.44%) followed by seed yield/plant (10.55%), siliquae length (5.5%), siliquae/plant (5%) and seeds/siliqua (1.66%). These progenies may be utilized for initiation of second cycle of selection/development of trait specific gene pools.
Rathore S.S.,Sewar |
Shekhawat K.,Sewar |
Premi O.P.,Sewar |
Kandpal B.K.,Sewar |
Indian Journal of Agronomy | Year: 2014
An experiment on standardization of micro-irrigation and fertigation methods for Indian mustard [Brassica juncea (L.) cernj. & Cosson] crop under semi-arid conditions of Rajasthan was undertaken during the winter (rabi) seasons of 2009–10 and 2010–11. The treatments consisted of 5 irrigation systems in main plot, viz. micro-sprinkler system (MS), micro sprinkler followed by check basin (MS+CB), drip irrigation system, drip system followed by check basin (DS+CB) and check basin (CB) alone. In sub-plots, 4 treatments of N doses, viz. control (0 kg/ha N), 40, 80 and 120 kg N/ha, were taken. Pressurized irrigation through micro-sprinkler, drip system and their combination with check basin method resulted in significantly higher growth attributes like plant height, above-ground dry matter and chlorophyll concentration than check basin alone. Similar trend was observed for biological, seed and oil yields. Other parameters like water-use efficiency and production efficiency were the highest under pressurized irrigation systems. Among the sub-plots, growth parameters and yield attributes improved with increasing dose of nitrogen up to 120 kg/ha. The enhanced yield under 120 kg N/ha also resulted in higher production efficiency and water-use efficiency. © 2014, Indian Society of Agronomy. All rights reserved.