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Singh M.,Kurukshetra University | Sukhvinder,Seth jai parkash mukand lal institute of engineering and technology | Kharab R.,Kurukshetra University
Nuclear Physics A | Year: 2013

We have analyzed the fusion excitation function data of various heavy ion systems in the near barrier energy region using the coupled channel code CCFULL and a new parameterization scheme for evaluating the depth of the Woods-Saxon nuclear potential. The potential model proposed here explains the fusion excitation function of the various heavy ion systems reasonably well. We have also discussed the role of projectile isotopic effects, target isotopic effects and the mass asymmetry in the entrance channel, by choosing the suitable projectile-target combinations, in the sub-barrier fusion enhancement. © 2012 Elsevier B.V. Source


Singh M.,Kurukshetra University | Sukhvinder,Seth jai parkash mukand lal institute of engineering and technology | Kharab R.,Kurukshetra University
Nuclear Physics A | Year: 2013

We have analyzed the fusion excitation function data of various heavy ion systems in the near barrier energy region using Wong's formula and an energy dependent potential. The various channel coupling effects for different projectile-target combinations are properly mocked up by the energy dependent potential resulting in a very good agreement between the data and the predictions. © 2012 Elsevier B.V. Source


Tyagi A.,National Dairy Research Institute | Kumar A.,Seth jai parkash mukand lal institute of engineering and technology | Aparna S.V.,National Dairy Research Institute | Mallappa R.H.,National Dairy Research Institute | And 2 more authors.
Critical Reviews in Food Science and Nutrition | Year: 2016

Synthetic biology also termed as “genomic alchemy” represents a powerful area of science that is based on the convergence of biological sciences with systems engineering. It has been fittingly described as “moving from reading the genetic code to writing it” as it focuses on building, modeling, designing and fabricating novel biological systems using customized gene components that result in artificially created genetic circuitry. The scientifically compelling idea of the technological manipulation of life has been advocated since long time. Realization of this idea has gained momentum with development of high speed automation and the falling cost of gene sequencing and synthesis following the completion of the human genome project. Synthetic biology will certainly be instrumental in shaping the development of varying areas ranging from biomedicine, biopharmaceuticals, chemical production, food and dairy quality monitoring, packaging, and storage of food and dairy products, bioremediation and bioenergy production, etc. However, potential dangers of using synthetic life forms have to be acknowledged and adoption of policies by the scientific community to ensure safe practice while making important advancements in the ever expanding field of synthetic biology is to be fully supported and implemented. © 2016, Copyright © Taylor & Francis Group, LLC. Source


Kumar A.,National Dairy Research Institute | Kumar A.,Seth jai parkash mukand lal institute of engineering and technology | Grover S.,National Dairy Research Institute | Batish V.K.,National Dairy Research Institute
Journal of General and Applied Microbiology | Year: 2013

A highly sensitive and specific multiplex PCR assay has been developed to detect the presence of Escherichia coli O157:H7 from naturally contaminated raw milk samples within 10 h. The primers explored in the assay were targeted against the uidR gene specific for all types of E. coli and the fliCH7 gene specific for the h7 flagellar antigen of E. coli O157:H7. The multiplex PCR assay developed was found to be highly specific as it produced PCR products of 152 bp (E. coli specific) and 625 bp (E. coli O157:H7 specific). The assay was tested for its specificity against different serotypes of E. coli as well as other pathogenic strains like Salmonella, Shigella, Klebsiella, Enterobacter, Staphylococcus aureus, Lactobacillus and Lactococcus etc. When this multiplex PCR assay was directly applied to 24 raw milk samples collected from different sources, E. coli O157:H7 could be detected in one of the milk samples without 4 h enrichment in CTSMAC broth and three samples after 4 h enrichment in CT-SMAC broth. However, all the pasteurized milk samples gave a negative signal for this organism. Source


Yadav A.K.,National Institute of Nutrition | Tyagi A.,National Dairy Research Institute | Kumar A.,Seth jai parkash mukand lal institute of engineering and technology | Saklani A.C.,Apeejay Stya University | And 2 more authors.
Archives of Microbiology | Year: 2014

Adhesion to the human intestinal epithelial cell is considered as one of the important selection criteria of lactobacilli for probiotic attributes. Sixteen Lactobacillus plantarum strains from human origins were subjected for adhesion to extracellular matrix (ECM) components, and their physiochemical characterization, incubation time course and effect of different pH on bacterial adhesion in vitro were studied. Four strains showed significant binding to both fibronectin and mucin. After pretreatment with pepsin and trypsin, the bacterial adhesion to ECM reduced to the level of 50 % and with lysozyme significantly decreased by 65–70 %. Treatment with LiCl also strongly inhibited (90 %) the bacterial adhesion to ECM. Tested strains showed highest binding efficacy at time course of 120 and 180 min. Additionally, the binding of Lp91 to ECM was highest at pH 6 (155 ± 2.90 CFU/well). This study proved that surface layer components are proteinaceous in nature, which contributed in adhesion of lactobacillus strains. Further, the study can provide a better platform for introduction of new indigenous probiotic strains having strong adhesion potential for future use. © 2014, Springer-Verlag Berlin Heidelberg. Source

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