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Alves A.N.L.,University of Sao Paulo | Sumita N.M.,Servico de Bioquimica Clinica | Fortini A.S.,Servico de Bioquimica Clinica | Neto M.P.,Servico de Bioquimica Clinica | And 2 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2010

Objective: The purpose of this study was to evaluate and improve a high performance liquid chromatography (HPLC) methodology to determine urinary δ-aminolevulinic acid (ALA-U) with small volumes of sample. Method: The method was based on the formation of a fluorescent compound and subsequent 15-minute chromatographic run. Results: The method shows suitable linearity, precision and recovery. Urine samples showed 1.2 ± 0.9 mg/l (media ± standard deviation) of ALA-U. Conclusion: The method was considered suitable for the routine analysis of ALA-U. Source

Mendes M.E.,University of Sao Paulo | Fagundes C.C.,BioMedica | Do Porto C.C.,Servico de Bioquimica Clinica | Bento L.C.,BioMedica | And 3 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2011

Water is a reagent used in most laboratory tests and, therefore, must follow stringent quality control standards. The urban water supply has organic molecules, inorganic ions, particles, colloids, gases, bacteria and their products, which may alter laboratory test results and cause occasional errors and mechanical failures in diagnostic equipment. To remove these impurities, it is necessary to use a combination of purification technologies. There are several organizations that specify reagent water standards to minimize its interference in laboratory assays. Most laboratories set standards established by the Clinical and Laboratory Standards Institute (CLSI), which classifies the type of water as follows: clinical laboratory reagent water (CLRW), special reagent water (SRW) and instrumental feed water (IFW). The quality monitoring is performed by means of assessing the resistivity, conductivity, total organic carbon (TOC), microbial control and endotoxins. The parameters are evaluated in accordance with the frequency determined by the standard used. In this article we discuss the importance of water employed in laboratory procedures, its quality control and its interference in laboratory assays. Source

Rodrigues E.,University of Sao Paulo | De Oliveira Lavorato L.M.,Servico de Bioquimica Clinica | Novais R.C.D.,Servico de Bioquimica Clinica | Bussmann L.Z.,DLC HC FMUSP | And 2 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2011

Introduction: Over the last century, mercury levels in the environment have tripled, increasing the risk of human contamination. Hence, it pollutes groundwater, rivers and seas, and it enters the food chain through the ingestion of contaminated fish. It affects the central nervous system and the inhalation of mercury vapors cause asthenia, fatigue, anorexia, weight loss and gastrointestinal disturbances. The contamination detection is carried out through hair and blood sample analysis. The replacement of mercury-containing equipment used in health services is crucial in order to reduce environmental and occupational hazards. Objective: To describe the implementation of "Zero Mercury" program at the Central Laboratory Division of University of São Paulo Medical School Hospital - CLD/USP-MSH (Divisão de Laboratório Central do Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo [DLC/HC-FMUSP]). Methods: The project was developed within the period of three years (2008-2010). During its elaboration, the action plans were defined, including a training program for the team responsible for the implementation of equipment and reagent replacement. Results: By the end of the project, CLD/USP-MSH received an honorable mention by the Brazilian Ministry of Labor and Employment for the fulfillment of "Mercury Zero" program requirements. Conclusion: A mercury-free laboratory environment requires managerial commitment, a consistent action plan and staff awareness of health and environmental risks. Source

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