Servico de Anatomia Patologica

Portugal

Servico de Anatomia Patologica

Portugal

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Menezes N.,Centro Hospitalar Of Vila Nova Of Gaia Chvng | Moreira A.,Centro Hospitalar Of Vila Nova Of Gaia Chvng | Mota G.,Centro Hospitalar Of Vila Nova Of Gaia Chvng | Tente D.,Servico de Anatomia Patologica | And 4 more authors.
Medicina Cutanea Ibero-Latino-Americana | Year: 2010

A 84 year-old man from north Portugal with chronic myelomonocytic leukaemia (diagnosed 2 years ago), under treatment with erythropoietin and hydroxyurea and Kaposi's sarcoma is reported. The patient was referred to our department with brown macules, papules and patches with a smooth surface and whitish scale, located to the lower limbs that had appeared 2 month ago. The diagnosis of Kaposi's sarcoma was histologically confirmed. He was HIV-1/HIV-2 negative and had no analytic detectable immussupression. The patient died short time after the diagnosis without specif any treatment.


Julio C.,Servico de Ginecologia | Amaral N.,Servico de Ginecologia | Biscaia I.,Servico de Anatomia Patologica | Torrezao I.,Servico de Anatomia Patologica | Fatela A.,Servico de Ginecologia
Acta Medica Portuguesa | Year: 2010

Background: Tuberculosis remains a global health problem, being the genitourinary tract the second most common site for tuberculosis infection after the lungs. Genital tuberculosis is now undergoing a worrying recrudescence. Case report: We report two cases of postmenopausal women who presented with vaginal bleeding. General physical and gynecological examination detected no abnormality. Transvaginal pelvic ultrasound showed fluid in the endometrial cavity in both cases. The office hysteroscopy was suggestive of focal endometrial thickness and an endometrial biopsy was performed. The histopathologic examination of biopsies found epithelioid cell granulomas without malignant cells. Culture of the endometrium was positive for Mycobacterium tuberculosis. Involvement of other systems was not detected. The patients started receiving antituberculosis treatment. Conclusion: Genital tuberculosis is rare in postmenopausal women and responsible for only approximately 1% of postmenopausal bleeding. However it is a curable disease and an early diagnosis is important and may prevent unnecessary invasive procedures for the patient. © 2010 CELOM.


Ueda E.K.,University of California at Riverside | Huang K.,University of California at Riverside | Nguyen V.,University of California at Riverside | Ferreira M.,Hospital Of Santa Maria | And 2 more authors.
Cell and Tissue Research | Year: 2011

Despite the important role of prolactin (PRL) in mammary gland development and function, little is known about the distribution of the different forms of the prolactin receptor (PRLR) under various physiological circumstances. Here, the distribution of the long (LF) and the short (S3 in mouse) receptor common to both mice and rats was determined by immunofluorescence on frozen sections of virgin, pregnant and lactating mouse mammary gland. Myoepithelial cells were consistently and intensely stained for both receptors. For luminal cells at all stages (ducts and alveoli), a large proportion of PRLR staining was unexpectedly present on the apical face. In the non-lactating state, no basal staining of luminal cells was detectable. During lactation, a proportion of both receptors moved to the basolateral surface. In vitro, HC11 cells showed constitutive expression of LF but expression of S3 only upon the formation of adherent junctions. Tight junction formation was accelerated by incubation in pseudo-phosphorylated PRL, as measured by transepithelial resistance and the expression and placement of the tight junction protein, zonula occludens-1. Once an intact monolayer had formed, all LF and S3 receptors were apical (akin to the non-lactating state) and only apical application of PRL activated the Jak2-STAT5 and ERK pathways. By contrast, basolateral application of PRL resulted in a reduction in basal ERK phosphorylation, suggesting an involvement of a dual specificity protein phosphatase. Normal human breast samples also showed apical PRLRs. These results demonstrate important contextual aspects of PRL-PRLR interactions with implications for the analysis of the role of PRL in breast cancer. © 2011 Springer-Verlag.


Cunha M.,Servico de Anatomia Patologica | Carneiro F.,Servico de Anatomia Patologica | Amil J.,Centro Hospitalar S Joao
Arquivos de Medicina | Year: 2013

Coeliac disease has a complex pathogenesis that results from the interaction between environmental (dietary exposure to gluten), genetic and immunologic factors. Gluten-free diet induces clinical and histological recovery in the majority of patients. However, a proportion of patients does not respond to a glutenfree diet and will be diagnosed as Refractory Coeliac Disease (RCD). RCD can be classified in type 1, characterized by polyclonal intraepithelial lymphocytes with a normal immune phenotype, and type 2, characterized by monoclonal intraepithelial lymphocytes with an aberrant immune phenotype. RCD is associated with a high risk of complications such as malnutrition, lymphocytic gastritis, ulcerative jejunitis and enteropathy-associated T-cell lymphoma, especially type 2. The prognosis is poor in RCD type 2, with a 5-year survival of approximately 50%. RCD can be considered a precursor of lymphoma. The treatment of RCD, mainly type 2, remains a clinical challenge.


Miranda L.H.M.,Laboratorio Of Pesquisa Clinica Em Dermatozoonoses Em Animais Domesticos | Quintella L.P.,Servico de Anatomia Patologica | Santos I.B.,Laboratorio Of Pesquisa Clinica Em Dermatozoonoses Em Animais Domesticos | Oliveira R.V.C.,Laboratorio Of Epidemiologia Clinica | And 3 more authors.
Journal of Comparative Pathology | Year: 2010

Pyogranulomatous lesions from 80 dogs with sporotrichosis and 26 dogs with American tegumentary leishmaniosis (ATL) were compared microscopically in order to identify features that would support the diagnostic suspicion and direct the subsequent search for the aetiological agent of either infection. Odds ratios and their respective 95% confidence intervals were calculated in order to evaluate the impact of the microscopical findings on the diagnosis of either disease. Lesions with well-formed granulomata were 14 times more likely to be due to sporotrichosis than ATL. Marked neutrophil infiltration into granulomata was 12.26 times more likely to be associated with sporotrichosis when compared with lesions having mild neutrophilic infiltration. Absence of lymphocytes and macrophages in the peripheral infiltrate was associated with a 9.71 and 4.93 higher chance, respectively, of being sporotrichosis rather than ATL compared with lesions where these cells were present. Lesions with a perivascular, perifollicular and interstitial peripheral inflammatory infiltrate were 5.48 times more likely to be due to sporotrichosis than ATL when compared with lesions with a diffuse peripheral infiltrate. Histopathological analysis may therefore contribute to the diagnosis of sporotrichosis or ATL skin lesions in dogs since this method permits the identification of features that direct the diagnostic suspicion, thus facilitating the search for the aetiological agent in histological sections, permitting the precise request of subsequent tests and thereby reducing costs and time taken to achieve a definitive diagnosis and the initiation of appropriate therapy. © 2009 Elsevier Ltd.


Pinto A.E.,Servico de Anatomia Patologica | Silva G.L.,University of Lisbon | Henrique R.,Servico de Anatomia Patologica | Henrique R.,Abel Salazar Biomedical Sciences Institute | And 8 more authors.
European Journal of Endocrinology | Year: 2014

Abstract Objective: Familial non-medullary thyroid cancer has been proposed as an aggressive clinical entity. Our aim in this study is to investigate potential distinguishing features as well as the biological and clinical aggressiveness of familial vs sporadic papillary thyroid carcinoma (PTC). We assessed clinicopathological characteristics, outcome measures and DNA ploidy. Design: A matched-case comparative study. Methods: A series of patients with familial PTC (nZ107) and two subgroups, one with three or more affected elements (nZ32) and another including index cases only (nZ61), were compared with patients with sporadic PTC (nZ107), matched by age, gender, pTNM disease extension and approximate follow-up duration. Histological variant, extrathyroidal extension, vascular invasion, tumour multifocality and bilateral growth were evaluated. Ploidy pattern was analysed in available samples by DNA flow cytometry. The probabilities of disease-free survival (DFS) and overall survival (OS) were estimated according to the Kaplan-Meier (K-M) method. Results: No patient with familial PTC died of disease during follow-up (median, 72 months), contrarily to five patients (4.7%) (PZ0.06) with sporadic PTC (median, 90 months). There was a significantly higher tumour multifocality in familial PTC (index cases subgroup) vs sporadic PTC (PZ0.035), and a trend, in the familial PTC cohort with three or more affected elements, to show extrathyroidal extension (PZ0.054) more frequently. No difference was observed in DNA ploidy status. The K-M analyses showed no significant differences between both entities in relation to DFS or OS. Conclusion: Apart from multifocality, familial PTC appears to have similar clinical/prognostic behaviour when compared with sporadic forms of the disease. © 2014 European Society of Endocrinology Printed in Great Britain.


Attygalle A.D.,Royal Marsden Hospital | Cabecadas J.,Servico de Anatomia Patologica | Gaulard P.,Groupe hospitalier Henri Mondor Albert Chenevier | Jaffe E.S.,U.S. National Cancer Institute | And 4 more authors.
Histopathology | Year: 2014

Mature T-cell and T/NK-cell neoplasms are both uncommon and heterogeneous, among the broad category of non-Hodgkin lymphomas. Owing to the lack of specific genetic alterations in the vast majority, most currently defined entities show overlapping morphological and immunophenotypic features, and therefore pose a challenge to the diagnostic pathologist. In the light of recent immunophenotypic, cytogenetic and molecular genetics advances in the field of T-cell and T/NK-cell lymphomas, the focus of the lymphoma workshop of the European Association for Haematopathology/Society for Hematopathology meeting in Lisbon, Portugal, in October 2012 was to refine existing diagnostic criteria and clarify the borders between overlapping entities. The panel reviewed over 200 submitted cases, which were grouped into five categories: (i) angioimmunoblastic T-cell lymphoma and T-follicular-helper-cell-associated lymphomas; (ii) CD30-positive T-cell lymphomas/lymphoproliferative diseases; (iii) extranodal T-cell and NK-cell neoplasms; (iv) EBV-associated T-cell/NK-cell lymphomas/lymphoproliferative diseases; and (v) peripheral T-cell lymphoma, not otherwise specified, post-transplant lymphoproliferative disorders, and mimics. This report summarizes the discussions and conclusions of the workshop, which question current diagnostic criteria and provide recommendations for refining existing classifications. © 2013 John Wiley & Sons Ltd.


PubMed | Servico de Anatomia Patologica.
Type: Journal Article | Journal: European journal of endocrinology | Year: 2013

Familial non-medullary thyroid cancer has been proposed as an aggressive clinical entity. Our aim in this study is to investigate potential distinguishing features as well as the biological and clinical aggressiveness of familial vs sporadic papillary thyroid carcinoma (PTC). We assessed clinicopathological characteristics, outcome measures and DNA ploidy.A matched-case comparative study.A series of patients with familial PTC (n=107) and two subgroups, one with three or more affected elements (n=32) and another including index cases only (n=61), were compared with patients with sporadic PTC (n=107), matched by age, gender, pTNM disease extension and approximate follow-up duration. Histological variant, extrathyroidal extension, vascular invasion, tumour multifocality and bilateral growth were evaluated. Ploidy pattern was analysed in available samples by DNA flow cytometry. The probabilities of disease-free survival (DFS) and overall survival (OS) were estimated according to the Kaplan-Meier (K-M) method.No patient with familial PTC died of disease during follow-up (median, 72 months), contrarily to five patients (4.7%) (P=0.06) with sporadic PTC (median, 90 months). There was a significantly higher tumour multifocality in familial PTC (index cases subgroup) vs sporadic PTC (P=0.035), and a trend, in the familial PTC cohort with three or more affected elements, to show extrathyroidal extension (P=0.054) more frequently. No difference was observed in DNA ploidy status. The K-M analyses showed no significant differences between both entities in relation to DFS or OS.Apart from multifocality, familial PTC appears to have similar clinical/prognostic behaviour when compared with sporadic forms of the disease.

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