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Cuernavaca, Mexico

Tellez-Sosa J.,Instituto Nacional Of Salud Publica | Rodriguez M.H.,Instituto Nacional Of Salud Publica | Gomez-Barreto R.E.,Instituto Nacional Of Salud Publica | Valdovinos-Torres H.,Instituto Nacional Of Salud Publica | And 7 more authors.
PLoS ONE | Year: 2013

Background: Influenza viruses display a high mutation rate and complex evolutionary patterns. Next-generation sequencing (NGS) has been widely used for qualitative and semi-quantitative assessment of genetic diversity in complex biological samples. The "deep sequencing" approach, enabled by the enormous throughput of current NGS platforms, allows the identification of rare genetic viral variants in targeted genetic regions, but is usually limited to a small number of samples. Methodology and Principal Findings: We designed a proof-of-principle study to test whether redistributing sequencing throughput from a high depth-small sample number towards a low depth-large sample number approach is feasible and contributes to influenza epidemiological surveillance. Using 454-Roche sequencing, we sequenced at a rather low depth, a 307 bp amplicon of the neuraminidase gene of the Influenza A(H1N1) pandemic (A(H1N1)pdm) virus from cDNA amplicons pooled in 48 barcoded libraries obtained from nasal swab samples of infected patients (n = 299) taken from May to November, 2009 pandemic period in Mexico. This approach revealed that during the transition from the first (May-July) to second wave (September-November) of the pandemic, the initial genetic variants were replaced by the N248D mutation in the NA gene, and enabled the establishment of temporal and geographic associations with genetic diversity and the identification of mutations associated with oseltamivir resistance. Conclusions: NGS sequencing of a short amplicon from the NA gene at low sequencing depth allowed genetic screening of a large number of samples, providing insights to viral genetic diversity dynamics and the identification of genetic variants associated with oseltamivir resistance. Further research is needed to explain the observed replacement of the genetic variants seen during the second wave. As sequencing throughput rises and library multiplexing and automation improves, we foresee that the approach presented here can be scaled up for global genetic surveillance of influenza and other infectious diseases. © 2013 Téllez-Sosa et al. Source

Canedo-Dorantes L.,Autonomous University of Morelos | Soenksen L.R.,Monterrey Institute of Technology | Garcia-Sanchez C.,Autonomous University of Morelos | Trejo-Nunez D.,Autonomous University of Morelos | And 8 more authors.
Archives of Medical Research | Year: 2015

Background and Aims: Cellular and animal models investigating extremely low frequency magnetic fields (ELF-MF) have reported promotion of leukocyte-endothelial interactions, angiogenesis, myofibroblast and keratinocyte proliferation, improvement of peripheral neuropathy and diabetic wound healing. In humans, it has also been reported that systemic exposure to ELF-MF stimulates peripheral blood mononuclear cells, promoting angiogenesis and healing of chronic leg ulcers. The aim of the study was to investigate the effect of exposing different blood volumes to specific ELF-MFs (120 Hz sinusoidal waves of 0.4-0.9 mT RMS) to induce healing of diabetic foot ulcers (DFUs). Methods: Twenty six diabetic patients with non-responsive DFUs were divided into two exposure groups to receive treatment and record healing time. The forearm group, exposed to ELF-MF 2 h/day, twice weekly (3.6 l of blood/session); and the thorax group, exposed 25 min/day, 2 times/week (162.5 l of blood/session). Treatment period was 100 days or upon complete healing. Ulcer recurrences and adverse effects were investigated during short-term (<1 year) and long-term (3.4-7.8 years) follow-up. Results: Mean healing time was 61.48 ± 33.08 days in the forearm group and 62.56 ± 29.33 days for the thorax group. No adverse effects or ulcer recurrences in the original ulcer site were reported during treatment, the short-term follow-up period or the long-term follow-up period in both groups. Conclusions: Healing time was independent of the amount of blood exposed to ELF-MF used in this trial. ELF-MFs are effective and safe and could be applied to non-healing DFUs in conjunction with other preventive interventions to reduce DFUs complications. © 2015 IMSS. Source

Villegas-Trejo A.,Servicios de Salud de Morelos | Che-Mendoza A.,Servicios de Salud de Morelos | Gonzalez-Fernandez M.,Servicios de Salud de Morelos | Guillermo-May G.,Autonomous University of Yucatan | And 5 more authors.
Salud Publica de Mexico | Year: 2011

Objective: To determine the relative importance of Aedes aegyti breeding sites for potential targeted dengue control interventions in Morelos. Material and Methods: Cross-sectional entomological surveys were conducted and collection of Ae. aegypti pupae was taken from all water-holding containers in Cuautla, Jojutla and Tlaquiltenango during dry (1 713 households) and rainy (1 677) seasons in 2008. Relative importance of different types of breeding sites was determined by the contribution (%) to total pupae production within each locality. Results: In Cuautla most pupae during the dry season were found in wash basins (48.5%), tanks and buckets/pots (15% each); during the rainy season, diverse small items (21.3%), buckets/pots (19.3%) and plant pots (12.9%) were more productive. In Jojutla and Tlaquiltenango, 97% of all pupae was found in plant pots during the dry season; during the rainy season diverse small items (26.3%), washing/cooking utensils (13.9%), buckets/pots (12.9%) and plant pots (12.7%) were significant. Conclusion: Prevention and control of the dengue vector should be based on this kind of evidence to target the most productive breeding-sites. Source

Villegas-Trejo A.,Servicios de Salud de Morelos | Manrique-Saide P.,University Autnoma Of Yucatn | Che-Mendoza A.,Servicios Estatales de Salud de Guerrero | Cruz-Canto W.,University Autnoma Of Yucatn | And 5 more authors.
Journal of the American Mosquito Control Association | Year: 2010

Aedes albopictus, Culex corniger, Cx. restuans, and Toxorhynchites theobaldi are reported for the first time for the Mexican State of Morelos. The updated list of species reported is also presented. © 2010 by The American Mosquito Control Association, Inc. Source

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