Servicio Nacional de Sanidad

Santa Cruz Tecamac, Mexico

Servicio Nacional de Sanidad

Santa Cruz Tecamac, Mexico
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Quezada-Salinas A.,Servicio Nacional de Sanidad | Moreno-Velazquez M.,Servicio Nacional de Sanidad | Cruz-Jaramillo J.L.,Servicio Nacional de Sanidad | Garcia-Avila C.J.,Servicio Nacional de Sanidad | Lopez-Buenfil J.A.,Servicio Nacional de Sanidad
Plant Disease | Year: 2017

Astrophytum ornatum (DC.) Britton & Rose, family Cactaceae, is native to Mexico and is categorized as a threatened species. It has a spherical stem at the beginning of its development, but during growth it becomes columnar. Wild specimens can reach up to 1 m high and 30 cm in diameter, although in cultivation they rarely exceed 30 cm in height. During September 2013, in Texcoco State, Mexico, dry rot symptoms were observed in 8-year-old A. ornatum plants grown in pots and in green spaces. Incidence was ∼30%. Symptoms began as small dots, olive in color, in the younger tissues of the aerial part of the plant; subsequently, they developed semicircular dry spots 3 to 5 cm in diameter, light brown, delimited by a light green margin. In some cases, lesions increased in size and coalesced, and after 240 days, 100% of the plant area was invaded. Eventually, affected tissues collapsed and the plants died. Inner tissue pieces were cut from the healthy-ill transition area, placed in potato dextrose agar (PDA), and incubated for 48 h at 22°C. Mycelium was grown out of infected plant tissue and transferred to PDA dishes. The developed colonies were purified by monoconidial cultures on PDA to determine their cultural and morphological characteristics (†). The isolates were identified as Fusarium oxysporum due to the production of sickle shaped macroconidia 30.4 μm long × 3.28 μm wide with three septa, foot-shaped basal cell and a short apical cell, in branched conidiophores or on the surface of sporodochia; microconidia developed in simple lateral phialides, oval to ellipsoid shaped, straight to curved, and without septa. The colonies were purple (†;†). Molecular analysis was performed on two isolates to validate morphological characterization. DNA was extracted from monoconidial cultures and partial ITS1-4 region EF1-α subunit fragments were amplified by PCR with universal primer sequences. BLASTn analysis performed to sequences showed 98 to 99% similarity with the corresponding F. oxysporum sequences deposited in the nr-nucleotide database (†), as well as with the Fusarium-ID database (†); the sequences were deposited as KX398010 (ITS1-4) and KY379852 (EF1-α), respectively. For pathogenicity tests, 4-year-old A. ornatum plants were inoculated with the KX398010 isolate using three methods: i) spraying spores, ii) wound + spraying spores, and iii) subepidermal injection of spores. For control plants, sterile distilled water was used. Wounding was done by causing lesions with a sterile needle. For each method of inoculation, five plants were used, and the pathogenicity test was repeated two times. The inoculation in the aerial part (ribs) of the plant was carried out because the symptoms begin in this zone. For spraying spores, the first symptoms of dry rot were observed 15 days after inoculation as olive colored spots, which developed semicircular dry light brown spots; in the spores’ injection method, the tissues collapsed and died, causing perforations; lastly, by wound plus spraying spores method, they developed circular light brown spots from the damage done. The symptoms were similar to those observed in the first plants with dry rot, which originated this research. F. oxysporum was recovered from infected tissues by reisolation. To our knowledge, this is the first report of F. oxysporum in A. ornatum plants in Mexico. This work has significance in nurseries as well as for the conservation of this cactus, which is a threatened species. © 2017, American Phytopathological Society. All rights reserved.

Cossio-Bayugar R.,Veterinaria Del Instituto Nacional Of Investigaciones Forestales | Miranda-Miranda E.,Veterinaria Del Instituto Nacional Of Investigaciones Forestales | Arreguin-Perez C.A.,Veterinaria Del Instituto Nacional Of Investigaciones Forestales | Lozano L.,Servicio Nacional de Sanidad | And 4 more authors.
Genome Announcements | Year: 2017

Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 contigs, an N50 of 179,476 bp, and 41.93% G+C content. © 2017 Cossío-Bayúgar et al.

Lopez R.N.,Servicio Nacional de Sanidad | Salinas L.V.,U.S. Department of Agriculture | Chavez S.A.,Autonomous University of Chiapas | Gonzalez I.L.,Servicio Nacional de Sanidad | And 2 more authors.
Revista Mexicana De Ciencias Pecuarias | Year: 2015

The present study aims to improve the surveillance system of vesicular diseases in Mexico, using the strategic planning system, by identifying endemic areas in which the New Jersey serotype viral lineages are stable and by analyzing the information generated during 32 yr of monitoring. We show the necessary elements for building the Matrix of Results Indicators (MRI) that could be used on the vesicular diseases surveillance program in Mexico and in other affected countries. The results that allowed the characterization of the epidemiological areas where the virus of vesicular stomatitis (VS) remains in a secular way in Mexico are presented. Elements for disease prevention are also provided in order to improve the live animal international trade.

Garcia-Avila C.D.J.,Servicio Nacional de Sanidad | Trujillo-Arriaga F.J.,Servicio Nacional de Sanidad | Lopez-Buenfil J.A.,Servicio Nacional de Sanidad | Gonzalez-Gomez R.,Servicio Nacional de Sanidad | And 5 more authors.
Florida Entomologist | Year: 2016

The 2 cryptic species of Euwallacea nr. Fornicatus (Coleoptera: Curculionidae: Scolytinae) have more than 300 known hosts, of which avocado is the most important. Fusarium dieback, the fungal disease associated with these beetles, has killed some host plant species in Israel and the USA. Kuroshio shot hole borer, one of the 2 cryptic species, is reported for the first time in Baja California, Mexico, and represents a new threat to avocado and other potential hosts in urban and natural areas.

PubMed | National Autonomous University of Mexico, Servicio Nacional de Sanidad and Autonomous University of the State of Morelos
Type: | Journal: SpringerPlus | Year: 2014

Endosulfan is an organochloride and persistent pesticide that has caused concern because of its impact in the environment and its toxicity to and bioaccumulation in living organisms. In this study, we isolated an endosulfan-degrading fungus from the activated sludge from an industrial wastewater treatment plant. Through repetitive enrichment and successive subculture in media containing endosulfan as the sole carbon source, a fungus designated CHE 23 was isolated. Based on a phylogenetic analysis, strain CHE 23 was assigned to the genus Penicillium sp. In a mineral salt medium with 50mg/l endosulfan as the sole source carbon, CHE 23 removed the added endosulfan in a period of six days. To verify the decrease in endosulfan toxicity due to the activity of the fungus, we performed genotoxicity tests trough the single cell gel electrophoresis assay or comet assay, with Eisenia fetida as the bioindicator species. This organism was exposed to the supernatants of the culture of the fungus and endosulfan. Our results indicated that the genotoxicity of endosulfan was completely reduced due the activity of this fungus. These results suggest that the Penicillium sp. CHE 23 strain can be used to degrade endosulfan residues and/or for water and soil bioremediation processes without causing toxicity problems, which are probably due to the generation of no-toxic metabolites during biodegradation.

Miranda-Miranda E.,National Research Center Disciplinaria en Parasitologia Veterinaria | Cossio-Bayugar R.,National Research Center Disciplinaria en Parasitologia Veterinaria | Martinez-Ibanez F.,Servicio Nacional de Sanidad | Bautista-Garfias C.R.,National Research Center Disciplinaria en Parasitologia Veterinaria
Medical and Veterinary Entomology | Year: 2011

Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Ixodida: Ixodidae) were infested by small Megaselia scalaris (Loew, 1866) (Diptera: Phoridae) flies. Larvae of this species exhibited opportunistic parasitism predominantly on engorged female ticks, causing severe damage to their cuticle through which the flies were able to reach R. microplus internal organs, on which they fed until developing into pupae in the tick's remains. The flies were kept by continuous propagation on fresh ticks over six generations during which the same parasitoid behaviour was observed. Here we report on an ixodid tick laboratory culture used for rearing M. scalaris. Medical and Veterinary Entomology © 2010 The Royal Entomological Society. No claim to original US government works.

PubMed | National Research Center Disciplinaria En Parasitologia Veterinaria Del Instituto Nacional Of Investigaciones Forestales Agricolas cuarias, National Autonomous University of Mexico and Servicio Nacional de Sanidad
Type: Journal Article | Journal: Genome announcements | Year: 2015

Paenibacillus larvae strain MEX14 is a facultative anaerobic endospore-forming bacterium that infects Apis mellifera larvae. Strain MEX14 was isolated from domestic bee larvae collected in a backyard in Mexico City. The estimated genome size was determined to be 4.18 Mb, and it harbors 4,806 protein coding genes (CDSs).

Saucedo-Carabez J.R.,Colegio de Mexico | Teliz-Ortiz D.,Colegio de Mexico | Ochoa-Ascencio S.,Universidad Michoacana de San Nicolás de Hidalgo | Ochoa-Martinez D.,Colegio de Mexico | And 2 more authors.
European Journal of Plant Pathology | Year: 2014

The total yield of cv. Hass and Mendez avocado trees was evaluated in Grove 1 in Uruapan county: four trees were sunblotch-infected, four asymptomatic (molecularly positive), and four healthy. A further four healthy and four symptomatic trees were evaluated in Grove 2 in Tingambato county. There was a significant 75 % reduction in the total Hass fruit weight from symptomatic trees in Grove 1 (213 kg vs 751 kg in healthy trees) and a 52 % reduction (124 kg vs 255 kg) in Grove 2 (Tukey, p = 0.05). This reduction was more severe in Mendez: 83 % in Grove 1 (183 kg vs 1,053 kg) and 76 % in Grove 2 (82 kg vs 340 kg). Yield of asymptomatic trees was reduced to 58 % in Mendez and 30 % in Hass. The average weight of 100 fruits was reduced by 13-28 % in Hass and 20-27 % in Mendez in Groves 2 and 1 respectively. The equatorial diameter of 100 symptomatic fruits was 8 % lower in Hass in both groves, and 20-28 % lower in Mendez. The polar diameter decreased by 8-10 % in Hass and 10-11 % in Mendez. The incidence of symptomatic fruits was 46-62 % in Hass and 20-28 % in Mendez. Yield of ASBVd symptomatic Hass and Mendez trees was significantly reduced and fruit morphology negatively affected. © 2013 KNPV.

Bertolini V.,Colegio de Mexico | Cruz-Blasi J.,Servicio Nacional de Sanidad | Damon A.,Colegio de Mexico | Mora J.V.,Colegio de Mexico
Acta Botanica Brasilica | Year: 2014

Orchids establish symbiosis with Rhizoctonia mycorrhizal fungi, forming the characteristic pelotons within the cells of the root cortex. Under natural conditions, terrestrial and epiphytic orchids have different levels of dependence upon the fungal symbiont, although various authors have mentioned that once orchid plants reach maturity the interaction becomes weaker and intermittent. Recent evidence shows that in some epiphytic orchid species mycorrhization is constant and systematic. In three species of wild orchids from southeast Mexico, we show that mycorrhization is systematically present in roots of different ages, in the wet and dry seasons. We demonstrate that the volume of the root that is colonized depends upon the quantity of rainfall and the diameter of the root, and that rainfall also determines the presence of fresh, undigested pelotons. In very thin roots, mycorrhizal colonization occupies a considerable proportion of the cortex, whereas in thicker roots the proportion of the volume of the root cortex colonized is lower. © 2014, Sociedade Botanica do Brasil. All rights reserved.

PubMed | Servicio Nacional de Sanidad
Type: Journal Article | Journal: American journal of veterinary research | Year: 2011

To characterize the temporal and spatial distribution and reproductive ratio of vesicular stomatitis (VS) outbreaks reported in Mexico in 2008.Bovine herds in Mexico in which VS outbreaks were officially reported and confirmed from January 1 through December 31, 2008.The Poisson model of the space-time scan statistic was used to identify periods and geographical locations at highest risk for VS in Mexico in 2008. The herd reproductive ratio (R(h)) of the epidemic was computed by use of the doubling-time method.1 significant space-time cluster of VS was detected in the state of Michoacan from September 4 through December 10, 2008. The temporal extent of the VS outbreaks and the value and pattern of decrease of the R(h) were different in the endemic zone of Tabasco and Chiapas, compared with findings in the region included in the space-time cluster.The large number of VS outbreaks reported in Mexico in 2008 was associated with the spread of the disease from the endemic zone in southern Mexico to areas sporadically affected by the disease. Results suggested that implementation of a surveillance system in the endemic zone of Mexico aimed at early detection of changes in the value of R(h) and space-time clustering of the disease could help predict occurrence of future VS outbreaks originating from this endemic zone. This information will help prevent VS spread into regions of Mexico and neighboring countries that are only sporadically affected by the disease.

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