Evaluation of in situ cromogenic hibridation compared with immunohistochemistry in breast cancer patients with overexpresion of Her2-neu [Evaluación de la hibridación in situ cromogénica comparado con inmunohistoquímica en mujeres con cáncer de mama con sobreexpresión del gen Her2-neu]
Valencia-Cedillo R.,Hospital Of Oncologia |
De Anda-Gonzalez J.,Hospital Of Oncologia |
Rodriguez-Cuevas S.,Servicio de Oncologia Quirurgica |
Aviles A.,Servicio de Patologia |
And 2 more authors.
Gaceta Mexicana de Oncologia | Year: 2012
Introduction: The gene of human epidermal growth factor 2 (Her2-neu), has prognostic implications in women with breast cancer. In our current practice, we determine overexpression of Her2-neu by immunohistochemistry (IHC). However, indeterminate results require in situ hybridization (ISH). The fluorescence in situ hybridization (FISH) is the gold standard to verify the amplification of the Her2-neu gene, however, this technique is expensive and difficult to do, so, we use Cromogenic in situ hybridization (CISH) as an alternative method. There are concerns regarding this technique has as good performance as FISH Our main goal was to evaluate the correlation between IHC and CISH for those cases with indeterminate results Material and methods: Paraffin blocks were selected from 114 women with breast cancer at two institutions, Her2-neu determination by IHC and CISH was performed later. The CISH results were based on the count of signals per nucleus and then correlated the results of both methods. Results: Correlation between both methods results was that of 98% , confirming gene amplification in 96% of patients that were positive (3+) with IHC, no amplification of the gene was found in 97% of the negative cases (0, 1+) and 37% of indeterminate cases (2+). Conclusions: The CISH technique proved to be a highly sensitive and specific technique in determining the amplification of the Her2-neu gene, especially in cases with indeterminate immunohistochemistry results (2+).
Ortiz-Mendoza C.M.,Servicio de Oncologia Quirurgica
Cirugia y Cirujanos | Year: 2010
Background: Textilomas may mimic a malignant neoplasm and may occur in rare locations. Clinical case: A 73-year-old female presented a groin tumor of 3 years duration after saphenectomy. Physical exam of the right groin area demonstrated an 8-cm oval tumor below the inguinal ligament and above the femoral vessels. An ultrasound and a CT scan showed a mixed cyst. During surgical exploration of the groin, an 8-cm tumor fixed to the femoral vein was extirpated en bloc. Histopathological results reported a textiloma. The patient had an uneventful postoperative evolution. Conclusions: Textilomas may mimic a neoplasm, but their occurrence in the groin is exceptional.
Total pelvic exenteration in gynecologic cancer. Analysis of prognosis factors and surgical results in the Centro Medico Nacional 20 de Noviembre of ISSSTE [Exenteración pélvica total en cáncer ginecológico. Análisis de factores pronóstico y resultados quirúrgicos en el Centro Médico Nacional 20 de Noviembre del ISSSTE]
Tenorio-Torres J.A.,Servicio de Oncologia Quirurgica |
Ortega-Meza B.A.,Servicio de Oncologia Quirurgica |
Cortes-Cardenas S.A.,Servicio de Oncologia Quirurgica
Gaceta Mexicana de Oncologia | Year: 2010
Background: Persistence or recurrence of carcinoma of the uterine cervix in previously treated patients has a poor prognosis. Rescue surgery is possible if there is central activity and it consists of pelvic exenteration. Objective: To identify risk factors and poor prognosis associated factors that influence survival in patients with gynecologic cancer recurrence in which pelvic excenteration was performed. Methods: Files from patients in which pelvic excenteration was performed in our service between January 2001 to December 2009. Results: Pelvic excenteration was performed in 19 patients with a medium of 53 years old, 8 with persistency and 11 with recurrence. The main factor which influenced overall survival was renal function deterioration and there is an association between time of renal disfunction and survival. There was no difference in survival when persistency and recurrency were compared (p = 0.53). There was no difference in survival depending on initial stage. Medial survival was 21.15 months. Conclusion: Results of the analysis showed that survival is determined by the presence of renal failure and the time of its presentation.
Malignant paraganglioma (multiple, multicentric and metastatic) in a female patient with family history of paraganglioma [Paraganglioma maligno (múltiple, multicéntrico y metastásico) en una paciente con historia familiar de paraganglioma]
Cobos Gonzalez E.,Centro Medico ABC |
Aragon Lopez J.A.,Centro Medico ABC |
Soria Cespedes D.R.,Centro Medico ABC |
de la Rosa Abaroa M.A.,Centro Medico ABC |
And 3 more authors.
Cirugia Espanola | Year: 2015
Malignant paragangliomas are rare, but may occur especially in patients with familial forms of the disease. We present the case of a 23 year old woman diagnosed with bilateral carotid paraganglioma with distant and local metastases, associated to a family history of paraganglioma and we present a literature review. © 2014 AEC.