Time filter

Source Type

Bilbao, Spain

Badaoui B.,Autonomous University of Barcelona | Manunza A.,Autonomous University of Barcelona | Castello A.,Autonomous University of Barcelona | D'Andrea M.,University of Molise | And 13 more authors.
Journal of Dairy Science

Inferring the breed of origin of dairy products can be achieved through molecular analysis of genetic markers with a population-specific pattern of segregation. The goal of the current work was to generate such markers in goats by resequencing several pigmentation genes [melanocortin 1 receptor (. MC1R), v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (. KIT), tyrosinase (. TYR), and tyrosinase-related protein 2 (. TYRP2)]. This experiment revealed 10 single nucleotide polymorphisms (SNP), including 5 missense mutations and 1 nonsense mutation. These markers were genotyped in 560 goats from 18 breeds originally from Italy, the Iberian Peninsula, the Canary Islands, and North Africa. Although the majority of SNP segregated at moderate frequencies in all populations (including 2 additional markers that were used as a source of information), we identified a c.764G>A SNP in MC1R that displayed highly divergent allelic frequencies in the Palmera breed compared with the Majorera and Tinerfeña breeds from the Canary Islands. Thus, we optimized a pyrosequencing-based technique that allowed us to estimate, very accurately, the allele frequencies of this marker in complex DNA mixtures from different individuals. Once validated, we applied this method to generating breed-specific DNA profiles that made it possible to detect fraudulent cheeses in which Palmero cheese was manufactured with milk from Majorera goats. One limitation of this approach, however, is that it cannot be used to detect illegal manufacturing where Palmero dairy products are produced by mixing milk from Palmera and Majorera goats, because the c.764G>A SNP segregates in both breeds. © 2014 American Dairy Science Association. Source

Martinez A.M.,University of Cordoba, Spain | Martinez A.M.,Northeast National University | Periati V.L.,University of Cordoba, Spain | Bermejo J.V.D.,University of Cordoba, Spain | And 29 more authors.
Journal of Animal Science

Little is known about local Criollo pig genetic resources and relationships among the various populations. In this paper, genetic diversity and relationships among 17 Criollo pig populations from 11 American countries were assessed with 24 microsatellite markers. Heterozygosities, F-statistics, and genetic distances were estimated, and multivariate, genetic structure and admixture analyses were performed. The overall means for genetic variability parameters based on the 24 microsatellite markers were the following: mean number of alleles per locus of 6.25 ± 2.3; effective number of alleles per locus of 3.33 ± 1.56; allelic richness per locus of 4.61 ± 1.37; expected and observed heterozygosity of 0.62 ± 0.04 and 0.57 ± 0.02, respectively; within-population inbreeding coefficient of 0.089; and proportion of genetic variability accounted for by differences among breeds of 0.11 ± 0.01. Genetic differences were not significantly associated with the geographical location to which breeds were assigned or their country of origin. Still, the NeighborNet dendrogram depicted the clustering by geographic origin of several South American breeds (Criollo Boliviano, Criollo of northeastern Argentina wet, and Criollo of northeastern Argentina dry), but some unexpected results were also observed, such as the grouping of breeds from countries as distant as El Salvador, Mexico, Ecuador, and Cuba. The results of genetic structure and admixture analyses indicated that the most likely number of ancestral populations was 11, and most breeds clustered separately when this was the number of predefined populations, with the exception of some closely related breeds that shared the same cluster and others that were admixed. These results indicate that Criollo pigs represent important reservoirs of pig genetic diversity useful for local development as well as for the pig industry. © 2014 American Society of Animal Science. All rights reserved. Source

Badaoui B.,Autonomous University of Barcelona | Manunza A.,Autonomous University of Barcelona | D'Andrea M.,University of Molise | Pilla F.,University of Molise | And 11 more authors.
Italian Journal of Animal Science

Tyrosinase-related protein 1 (TYRP1) has been shown to play a fundamental role in pigmentation both in human and mouse. In this work, we aimed to characterize the variability of the caprine TYRP1 gene and investigate its segregation in a wide array of goat breeds. By partially sequencing the coding region of the TYRP1 gene in 18 individuals from eight different breeds, we were able to identify a synonymous nucleotide substitution at exon 3 (c.483C>T). An extensive survey of Iberian and Balearic (N=175), Italian (N=99), Swiss (N=54), Asian (N=14), Canarian (N=92) and North African (N=117) goats with different coat colours was carried out. We found that the C-allele has a different distribution in European vs African breeds, being almost fixed in the latter. Moreover, the C-allele showed an increased frequency in white coated breeds (Girgentana, Grigia Molisana, Blanca de Rasquera and Saanen) when compared with those displaying a dark pigmentation (Cilentana Nera, Azpi Gorri and Murciano-Granadina). This could be due to genetic drift, migration and other factors associated with the demographic history of breeds under analysis or to a genetic hitchhiking event (c.483C>T frequencies would be shaped by a neighbouring causal mutation differentially selected in white and black goats). More refined studies will be needed to distinguish between these two alternative explanations. © B. Badaoui et al., 2012. Source

Schulz U.,Camino del Aleman 5 | Tupac-Yupanqui I.,Complutense University of Madrid | Martinez A.,University of Cordoba, Spain | Mendez S.,Complutense University of Madrid | And 4 more authors.

The domestic camel (dromedary) is the most important livestock species in the Canary Islands and the most important autochthonous European camel population. After six centuries of a successful adaptation process to the particular environment of the Canary Islands, the abandonment of traditional agriculture has led this population to a major bottleneck. Along with a lack of foreign genetic interchanges, this could lead the population to the brink of extinction. Genetic analysis using 13 microsatellites showed the closest genetic proximity to the North African (Tindouf, Algeria) camel population and a certain degree of sub-division, with significant genetic differences among breeders. An important level of genetic differentiation among the different populations analyzed was found with a global F ST value of 0.116. © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. Source

Martinez A.,University of Cordoba, Spain | Ferrando A.,Autonomous University of Barcelona | Manunza A.,Autonomous University of Barcelona | Gomez M.,Servicio de Ganaderia | And 7 more authors.
Small Ruminant Research

Demographic processes leave specific genetic signatures that can be detected through the analysis of nuclear and mitochondrial markers. Often, these signatures are very complex to interpret due to the co-occurrence of processes that are very distinct in nature and magnitude. In this work, we have analysed the footprint of past bottlenecks in the gene pool of Azpi Gorri goats, a Basque breed in a critical status of conservation because of dramatic reductions in its census. A sample of Azpi Gorri goats was analysed by sequencing the mitochondrial control region (N=24) and genotyping 29 autosomal microsatellites (N=50). Contrary to our expectations, the analysis of nuclear and mitochondrial markers showed that the Azpi Gorri breed has retained a considerable level of genetic diversity. Even more, conflicting results were obtained when trying to detect bottleneck genetic signatures through the calculation of R 2 and Fu's F S statistics (mitochondrial data) and by performing Bottleneck and M_P_VAL analyses (microsatellite data). Cryptic bottleneck genetic signatures might be relatively frequent in domestic species because population declines are usually smooth and sustained, rather than drastic and instantaneous, and also because many concomitant factors, such as population subdivision, selection and migration, can alter their shape. © 2011 Elsevier B.V. Source

Discover hidden collaborations