Servicio de Analisis Clinicos

San Cristóbal de Segovia, Spain

Servicio de Analisis Clinicos

San Cristóbal de Segovia, Spain
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Herrera-Peco I.,Hospital Universitario La Paz | Herrera-Peco I.,University of San Pablo - CEU | Pastor J.,Servicio de Neurofisiologia Clinica | Pastor J.,University of San Pablo - CEU | And 3 more authors.
Revista de Neurologia | Year: 2011

Introduction. Temporal lobe epilepsy (TLE) is commonly associated with the process of synchronisation during the interictal stage, which show up as 'spikes' on neurophysiological recordings, and also with hypersynchronic activity during clinical seizures. Nevertheless, desynchronisation also seems to play an important role in the epileptogenic process, favouring the onset of seizures. Aims. The aim of this work is to show how the latest complex network analysis techniques applied to the recordings from the foramen ovale electrodes provide valuable new information about the dynamics of mesial activity in TLE. The study also seeks to show that desynchronisation of the mesial activity plays an important role in TLE. Patients and methods. A cluster technique was used to analyse the recordings of six patients with TLE during the interictal stage and two seizures during the ictal period. Results. Electrical activity on the ipsilateral side behaves in a less synchronic manner than that of the contralateral side. There is clearly a greater tendency in the mesial zone of the epileptic side to arrange itself in isolated groups of synchronic activity than on the contralateral side, which is organised in large groups of synchronised activity. Conclusions. Analysis of the neurophysiological recordings, especially from the foramen ovale electrodes, by cluster and network analysis provides novel information that is not accessible by classical spike analysis. The greater degree of desynchronisation on the ipsilateral side would favour the appearance and origin of the seizures on that side. © 2011 Revista de Neurología.


El Assar M.,Institute Investigacion Sanitaria Of Getafe Getafe | Santos-Ruiz M.,Servicio de Analisis Clinicos | Ruiz de Adana J.C.,Servicio de Cirugia General y del Aparato Digestivo | Pindado M.L.,Servicio de Anestesiologia y Reanimacion | And 3 more authors.
Journal of Physiology | Year: 2016

Insulin resistance (IR) is determinant for endothelial dysfunction in human obesity. Although we have previously reported the involvement of mitochondrial superoxide and inflammation, other mechanisms could compromise NO-mediated responses in IR. We evaluated the role of the endogenous NOS inhibitor asymmetric dimethylarginine (ADMA) and arginase with respect to IR-induced impairment of l-arginine/NO-mediated vasodilatation in human morbid obesity and in a non-obese rat model of IR. Bradykinin-induced vasodilatation was evaluated in microarteries derived from insulin-resistant morbidly obese (IR-MO) and non-insulin-resistant MO (NIR-MO) subjects. Defective endothelial vasodilatation in IR-MO was improved by l-arginine supplementation. Increased levels of ADMA were detected in serum and adipose tissue from IR-MO. Serum ADMA positively correlated with IR score and negatively with pD2 for bradykinin. Gene expression determination by RT-PCR revealed not only the decreased expression of ADMA degrading enzyme dimethylarginine dimethylaminohydrolase (DDAH)1/2 in IR-MO microarteries, but also increased expression of arginase-2. Arginase inhibition improved endothelial vasodilatation in IR-MO. Analysis of endothelial vasodilatation in a non-obese IR model (fructose-fed rat) confirmed an elevation of circulating and aortic ADMA concentrations, as well as reduced DDAH aortic content and increased aortic arginase activity in IR. Improvement of endothelial vasodilatation in IR rats by l-arginine supplementation and arginase inhibition provided functional corroboration. These results demonstrate that increased ADMA and up-regulated arginase contribute to endothelial dysfunction as determined by the presence of IR in human obesity, most probably by compromising arginine availability. The results provide novel insights regarding the mechanisms of endothelial dysfunction related to obesity and IR and establish potential therapeutic targets for intervention. Key points: The presence of insulin resistance (IR) is determinant for endothelial dysfunction associated with obesity. Although recent studies have implicated the involvement of mitochondrial superoxide and inflammation in the defective nitric oxide (NO)-mediated responses and subsequent endothelial dysfunction in IR, other mechanisms could compromise this pathway. In the present study, we assessed the role of asymmetric dimethylarginine (ADMA) and arginase with respect to IR-induced impairment of endothelium-dependent vasodilatation in human morbid obesity and in a non-obese rat model of IR. We show that both increased ADMA and up-regulated arginase are determinant factors in the alteration of the l-arginine/NO pathway associated with IR in both models and also that acute treatment of arteries with arginase inhibitor or with l-arginine significantly alleviate endothelial dysfunction. These results help to expand our knowledge regarding the mechanisms of endothelial dysfunction that are related to obesity and IR and establish potential therapeutic targets for intervention. Journal compilation © 2016 The Physiological Society.


PubMed | Institute Investigacion Sanitaria Of Getafe, Servicio de Cirugia General y del Aparato Digestivo., Hospital Universitario Ramon y Cajal, Servicio de Anestesiologia y Reanimacion. and Servicio de Analisis Clinicos.
Type: Journal Article | Journal: The Journal of physiology | Year: 2016

The presence of insulin resistance (IR) is determinant for endothelial dysfunction associated with obesity. Although recent studies have implicated the involvement of mitochondrial superoxide and inflammation in the defective nitric oxide (NO)-mediated responses and subsequent endothelial dysfunction in IR, other mechanisms could compromise this pathway. In the present study, we assessed the role of asymmetric dimethylarginine (ADMA) and arginase with respect to IR-induced impairment of endothelium-dependent vasodilatation in human morbid obesity and in a non-obese rat model of IR. We show that both increased ADMA and up-regulated arginase are determinant factors in the alteration of the l-arginine/NO pathway associated with IR in both models and also that acute treatment of arteries with arginase inhibitor or with l-arginine significantly alleviate endothelial dysfunction. These results help to expand our knowledge regarding the mechanisms of endothelial dysfunction that are related to obesity and IR and establish potential therapeutic targets for intervention.Insulin resistance (IR) is determinant for endothelial dysfunction in human obesity. Although we have previously reported the involvement of mitochondrial superoxide and inflammation, other mechanisms could compromise NO-mediated responses in IR. We evaluated the role of the endogenous NOS inhibitor asymmetric dimethylarginine (ADMA) and arginase with respect to IR-induced impairment of l-arginine/NO-mediated vasodilatation in human morbid obesity and in a non-obese rat model of IR. Bradykinin-induced vasodilatation was evaluated in microarteries derived from insulin-resistant morbidly obese (IR-MO) and non-insulin-resistant MO (NIR-MO) subjects. Defective endothelial vasodilatation in IR-MO was improved by l-arginine supplementation. Increased levels of ADMA were detected in serum and adipose tissue from IR-MO. Serum ADMA positively correlated with IR score and negatively with pD2 for bradykinin. Gene expression determination by RT-PCR revealed not only the decreased expression of ADMA degrading enzyme dimethylarginine dimethylaminohydrolase (DDAH)1/2 in IR-MO microarteries, but also increased expression of arginase-2. Arginase inhibition improved endothelial vasodilatation in IR-MO. Analysis of endothelial vasodilatation in a non-obese IR model (fructose-fed rat) confirmed an elevation of circulating and aortic ADMA concentrations, as well as reduced DDAH aortic content and increased aortic arginase activity in IR. Improvement of endothelial vasodilatation in IR rats by l-arginine supplementation and arginase inhibition provided functional corroboration. These results demonstrate that increased ADMA and up-regulated arginase contribute to endothelial dysfunction as determined by the presence of IR in human obesity, most probably by compromising arginine availability. The results provide novel insights regarding the mechanisms of endothelial dysfunction related to obesity and IR and establish potential therapeutic targets for intervention.


Puiguriguer Ferrando J.,Servicio de Urgencias | Barcelo Martin B.,Servicio de Analisis Clinicos | Castanyer Puig T.,Servicio de Analisis Clinicos | Castanyer Puig T.,Hospital Universitario Son Dureta
Emergencias | Year: 2010

The Rumack-Matthew nomogram is used to guide treatment with N-acetylcysteine (NAC) as an antidote for paracetamol intoxication, but this approach has limitations. The elimination half-life of paracetamol increases to over 4 hours in the presence of hepatic toxicity. The aim of this study is to describe the characteristics of a series of cases of paracetamol toxicity in which a nomogram could not be used; the patient's risk was assessed by means of the elimination half-life. Patients with paracetamol intoxication from a single or cumulative overdose who came to Hospital de Son Dureta de Palma de Mallorca and Hospital Clínic de Barcelona over a 5-year period (July 2005-July 2010) were included if the findings of at least 2 immunoassays for paracetamol concentration were available. Half-life was estimated by means of the ratio between 2 consecutive concentrations determined 2 hours apart. Of 11 patients with paracetamol intoxication for whom a Rumack-Matthew nomogram was not used, 3 had liver toxicity. Two of them were accidental intoxications caused by ingesting several doses of the drug. The third was an attempted suicide. In all cases, the elimination half-life exceeded 4 hours. We suggest that in cases of paracetamol intoxication the Rumack- Matthew nomogram be complemented by calculation of the elimination half-life, which will provide an indication of possible liver toxicity in these patients. This approach should be chosen at least when there are doubts about when paracetamol ingestion occurred or if intoxication is the result of several doses.


Rodriguez F.,University of Murcia | Nieto-Ceron S.,Servicio de Analisis Clinicos | Fenoy F.J.,University of Murcia | Lopez B.,University of Murcia | And 4 more authors.
American Journal of Physiology - Regulatory Integrative and Comparative Physiology | Year: 2010

Females. suffer a less severe ischemic acute renal failure than males, apparently because of higher nitric oxide (NO) bioavailability and/or lower levels of oxidative stress. Because the renal ischemic injury is associated with outer medullary (OM) endothelial dysfunction, the present study evaluated sex differences in OM changes of NO and peroxynitrite levels (by differential pulse voltammetry and amperometry, respectively) during 45 min of ischemia and 60 min of reperfusion in anesthetized Sprague-Dawley rats. Endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) protein expression and their phosphorylated forms [peNOS(Ser1177) and pnNOS(Ser1417)], 3-nitrotyrosine, reduced sulfhydryl groups (-SH), and glomerular filtration rate (GFR) were also determined. No sex differences were observed in monomeric eNOS and nNOS expression, NO, or 3-nitrotyrosine levels in nonischemic kidneys, but renal -SH content was higher in females. Ischemia increased dimeric/monomeric eNOS and nNOS ratio more in females, but the dimeric phosphorylated peNOS(Ser1177) and pnNOS(Ser1417) forms rose similarly in both sexes, indicating no sex differences in nitric oxide synthase activation. However, NO levels increased more in females than in males (6,406.0 ± 742.5 and 4,058.2 ± 272.35 nmol/l respectively, P < 0.05), together with a lower increase in peroxynitrite current (5.5 ± 0.7 vs. 12.7 ± 1.5 nA, P < 0.05) and 3-nitrotyrosine concentration, (28.7 ± 3.7 vs. 48.7 ± 3.7 nmol/mg protein, P < 0.05) in females than in males and a better preserved GFR after ischemia in females than in males (689.7 ± 135.0 and 221.4 ± 52.5 μl·min-1·g kidney wt -1, P < 0.01). Pretreatment with the antioxidants N-acetyl-L-cysteine or ebselen abolished sex differences in peroxynitrite, nitrotyrosine, and GFR, suggesting that a greater oxidative and nitrosative stress worsens renal damage in males. Copyright © 2010 the American Physiological Society.


Julia-Serda G.,Hospital Universitario Dr Negrin | Cabrera-Navarro P.,Hospital Universitario Dr Negrin | Acosta-Fernandez O.,Hospital Nuestra Senora Of La Candelaria | Martin-Perez P.,Hospital Universitario Dr Negrin | And 4 more authors.
International Journal of Tuberculosis and Lung Disease | Year: 2011

OBJECTIVE: To evaluate the prevalence of and risk factors for asthma and related conditions in the Canaries, Spain. METHODS: From a randomised sample of 9506 adults aged 20-44 years who answered a short questionnaire, a random sample corresponding to 20% of the original was taken. Subjects classified as symptomatic in the previous survey and who were not included in the random sample were also invited to participate. The subjects completed a respiratory questionnaire, and underwent spirom etry, bronchial hyperresponsiveness (BHR) test, skin tests and immunoglobulin E (IgE) measurements. RESULTS: The random sample included 593 subjects. The prevalence of skin sensitisation to mites was 30.3% (95%CI 26.7-34.2) and the prevalence of IgE to mites 30.5% (95%CI 26.2-35.2). A prevalence of 40.6% (95%CI 35.9-45.5) was found for atopy, 14.1% (95%CI 11.1-17.1) for BHR and 4.2% (95%CI, 2.5-5.9) for asthma. The risk factors most strongly associated with asthma were atopy (OR 4.89, 95%CI 3.07-7.78) and respiratory infection before the age of 5 years (OR 2.78, 95%CI 1.66-4.67). CONCLUSION: This study shows a high prevalence of sensitisation to mites, atopy, BHR and asthma in the Canaries, similar to that observed in English-speaking countries. We suggest that these findings could partially result from climatic conditions. © 2011 The Union.


Jesus Hermida F.,Servicio de Analisis Clinicos | Fernandez M.,Servicio de Analisis Clinicos | Laborda B.,Hospital Of Cabuenes | Perez A.,Servicio de Analisis Clinicos | And 2 more authors.
Clinical Laboratory | Year: 2012

Background: The aim of the present study was to evaluate some analytical performances of the ADVIA Centaur® analyzer for the quantitative measurement of 25-OH Vitamin D [25(OH)D] in serum. Methods: Serum concentrations of 25(OH)D were determined by a new automated chemiluminescence immunoassay method introduced by Siemens and adapted to an ADVIA Centaur® analyzer, and compared with HPLC and a commercial chemiluminescence immunoassay (Liaison® DiaSorin). Results: The assay displayed a low intra-day (CV < 7.0%) and a low inter-day imprecision (CV < 9.0%). The ADVIA Centaur® demonstrated a stronger Spearman's correlation (r = 0.921), better agreement (bias = -0.3 ng/mL), and better concordance correlation coefficient of Lin (pc = 0.88) and better kappa index (k = 0.92) with HPLC, than the Liaison® DiaSorin assay (r = 0.907, bias = 5.9 ng/mL, pc = 0.84, k = 0.60) with HPLC. On the other hand, a significant inverse relationship was observed between parathyroid hormone (PTH) and 25(OH)D concentrations. Conclusions: The ADVIA Centaur® method is reliable for routine 25(OH)D determination in clinical laboratories.


Hermida F.J.,Servicio de Analisis Clinicos | Lorenzo M.J.,Servicio de Analisis Clinicos | Perez A.,Servicio de Analisis Clinicos | Fernandez M.,Servicio de Analisis Clinicos | And 2 more authors.
Scandinavian Journal of Clinical and Laboratory Investigation | Year: 2014

Objective. The aim of this study was two-fold: Firstly, to compare the serum creatinine concentration measured by enzymatic method and uncompensated kinetic Jaffe method, and secondly, to compare the effects of certain interfering substances such as glucose, bilirubin, proteins, triglycerides and hemoglobin on creatinine measurement. Methods. The determination of serum creatinine concentrations by enzymatic method and uncompensated kinetic Jaffe method was performed on ADVIA® 2400 analyzer. The interfering substances were tested by adding solutions of interference to serum pool with low (62 μmol/L), medium (221 μmol/L) and elevated (486 μmol/L) creatinine concentration. Results. In the method comparison study, despite the fact that the correlation between both methods for determining serum creatinine is very good (r = 0.998, p < 0.001), the regression analysis revealed that the results are not transferable, as indicated by the slope and intercept, which are significantly different from 1 and 0, respectively. A positive bias of + 14.1% in the determination of serum creatinine by uncompensated kinetic Jaffe method was found, and when the creatinine value is lower than ∼ 180 μmol/L this difference or bis progressively increases. We found a significant positive interference due to proteins and glucose and a significant negative interference due to bilirubin by kinetic Jaffe method, and no interferences by enzymatic method were found. Conclusions. In conclusion, the enzymatic method is the best choice for determining serum creatinine with the ADVIA® 2400 analyzer. © 2014 Informa Healthcare.


PubMed | Servicio de Analisis Clinicos
Type: Comparative Study | Journal: Scandinavian journal of clinical and laboratory investigation | Year: 2014

The aim of this study was two-fold: Firstly, to compare the serum creatinine concentration measured by enzymatic method and uncompensated kinetic Jaffe method, and secondly, to compare the effects of certain interfering substances such as glucose, bilirubin, proteins, triglycerides and hemoglobin on creatinine measurement.The determination of serum creatinine concentrations by enzymatic method and uncompensated kinetic Jaffe method was performed on ADVIA 2400 analyzer. The interfering substances were tested by adding solutions of interference to serum pool with low (62 mol/L), medium (221 mol/L) and elevated (486 mol/L) creatinine concentration.In the method comparison study, despite the fact that the correlation between both methods for determining serum creatinine is very good (r = 0.998, p < 0.001), the regression analysis revealed that the results are not transferable, as indicated by the slope and intercept, which are significantly different from 1 and 0, respectively. A positive bias of + 14.1% in the determination of serum creatinine by uncompensated kinetic Jaffe method was found, and when the creatinine value is lower than 180 mol/L this difference or bis progressively increases. We found a significant positive interference due to proteins and glucose and a significant negative interference due to bilirubin by kinetic Jaffe method, and no interferences by enzymatic method were found.In conclusion, the enzymatic method is the best choice for determining serum creatinine with the ADVIA 2400 analyzer.


PubMed | Servicio de Analisis Clinicos
Type: Comparative Study | Journal: Clinical laboratory | Year: 2012

The aim of the present study was to evaluate some analytical performances of the ADVIA Centaur analyzer for the quantitative measurement of 25-OH Vitamin D [25(OH)D] in serum.Serum concentrations of 25(OH)D were determined by a new automated chemiluminescence immunoassay method introduced by Siemens and adapted to an ADVIA Centaur analyzer, and compared with HPLC and a commercial chemiluminescence immunoassay (Liaison DiaSorin).The assay displayed a low intra-day (CV < 7.0%) and a low inter-day imprecision (CV < 9.0%). The ADVIA Centaur demonstrated a stronger Spearmans correlation (r = 0.921), better agreement (bias = -0.3 ng/mL), and better concordance correlation coefficient of Lin (P(c) = 0.88) and better kappa index (k = 0.92) with HPLC, than the Liaison DiaSorin assay (r = 0.907, bias = 5.9 ng/mL, p(c) = 0.84, k = 0.60) with HPLC. On the other hand, a significant inverse relationship was observed between parathyroid hormone (PTH) and 25(OH)D concentrations.The ADVIA Centaur method is reliable for routine 25(OH)D determination in clinical laboratories

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