Madrid, Spain
Madrid, Spain

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Sastre J.,Servicio de Alergologia | Sastre J.,CIBER ISCIII
Allergy: European Journal of Allergy and Clinical Immunology | Year: 2010

Basophil activation as a diagnostic tool in hydrochlorothiazide-induced noncardiogenic pulmonary edema. © 2009 John Wiley & Sons A/S.


Ballmer-Weber B.K.,University of Zürich | Skamstrup Hansen K.,Copenhagen University | Sastre J.,Servicio de Alergologia | Andersson K.,Phadia AB | And 8 more authors.
Allergy: European Journal of Allergy and Clinical Immunology | Year: 2012

Background Carrot is a frequent cause of food allergy in Europe. The objective of this study was to evaluate a panel of carrot allergens for diagnosis of carrot allergy in Spain, Switzerland and Denmark. Methods Forty-nine carrot allergic patients, 71 pollen allergic but carrot-tolerant patients and 63 nonatopic controls were included. Serum IgE to carrot extract, recombinant carrot allergens (rDau c 1.0104; rDau c 1.0201; rDau c 4; the isoflavone reductase-like proteins rDau c IFR 1, rDau c IFR 2; the carrot cyclophilin rDau c Cyc) were analyzed by ImmunoCAP. Results The sensitivity of the carrot extract-based test was 82%. Use of the recombinant allergens increased the sensitivity to 90%. The Dau c 1 isoforms were major allergens for Swiss and Danish carrot allergic patients, the profilin rDau c 4 for the Spanish patients. The rDau c IFR 1 and rDau c IFR 2 were recognized by 6% and 20% of the carrot allergics, but did not contribute to a further increase of sensitivity. Among pollen allergic controls, 34% had IgE to carrot extract, 18% to each of rDau c 1.0104, rDau c 1.0201 and rDau c 4, 8% to rDau c IFR 1 and 7% to rDau c IFR 2. Sensitization to rDau c Cyc occurred in one carrot allergic patient and one nonatopic control. Conclusion Component-resolved in vitro analyses revealed a significant difference in IgE sensitization pattern between geographical regions and in the prevalence of sensitization to carrot components between carrot allergic and carrot-tolerant but pollen sensitized patients. © 2012 John Wiley & Sons A/S.

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