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Ozzano dell'Emilia, Italy

Fernandez-Flores A.,Service of Anatomic Pathology | A Manjon J.,Service of Dermatology
Acta Dermatovenerologica Croatica | Year: 2010

Plasma cells have been considered as an important morphological clue in the diagnosis of some sclerosing cutaneous diseases such as morphea, so much so that humoral immunology has been suggested to have a role in the pathogenesis of such diseases. Nevertheless, they are hardly ever described as a prominent feature in lipodermatosclerosis in which granulocytes have been claimed as the main pathogenic cell. We report a case of lipodermatosclerosis in a 77-year-old woman, in which plasma cells were abundant in the thickened fibrous septa of the hypodermis. They were highlighted on immunohistochemical study with CD79a, CD138 and EMA, and showed polyclonal immunoexpression of kappa and lambda immunoglobulin light chains.

Fernandez-Flores A.,Service of Anatomic Pathology
American Journal of Dermatopathology | Year: 2010

A retrospective study of all cases of cutaneous metastases received in the Department of Pathology during the last 13 years was performed. Seventy-eight cases from 69 patients were examined. The majority of the patients were between 60 and 80 years of age. The most frequent anatomical location for the metastases was the abdomen. Regarding the primary tumor, breast carcinoma was the most common. In females breast carcinoma was the most common, while lymphomas predominated in males. In 14 patients (17.95%), the origin of the primary tumor was unknown. In all these cases, there was no clinical suspicion of metastasis. Epidermotropism was found in only 9 out of 78 cases (11.54%). Contrary to this, vascular invasion was a common feature, being present in 49 out of 78 cases (62.82%). Necrosis was evident in 38 cases (48.72%), but it was either moderate or prominent only in 8 cases (10.26%). Inflammatory infiltrate was absent in 3 cases (3.85%), mild in 49 (62.82%), moderate in 20 (25.64%), and prominent (graded either 3 of 4 or 4 out of 4) in 5 cases (6.41%). The metastases involved exclusively the dermis in 26 cases (33.33%), hypodermis in 7 cases (8.97%), and dermis and hypodermis in 44 cases (56.41%). We concluded that vascular invasion is a common feature in cutaneous metastases, especially in nonhematologic neoplasms, and because sometimes is focal, it should be carefully searched. Copyright © 2010 by Lippincott Williams & Wilkins.

Fernandez-Flores A.,Service of Anatomic Pathology
Journal of Clinical Pathology | Year: 2011

In October 1859, 'The Queen of Hearts' was published by Wilkie Collins. This book is a collection of 10 short stories written by the protagonists (three retired brothers), in order to keep their niece (aka the queen of hearts) entertained, until the arrival of her fiancé-to-be. The second story of the book, titled 'Brother Griffith's story of the family secret,' narrates the death of a young girl who has a slow-growing deforming tumour on her neck. Even with the few clinical details provided by the literary description, the current report examines the possibility of Hodgkin lymphoma as a diagnosis, concluding that the option is not an unwise one.

Fernandez-Flores A.,Service of Cellular Pathology | Valerdiz S.,Service of Anatomic Pathology | Crespo L.G.,Service of General Surgery | Rodriguez R.,Service of General Surgery
Acta Dermatovenerologica Croatica | Year: 2011

Injected corticosteroids can sometimes lead to a granulomatous reaction. This is apparently also true for anabolic steroids, which are often used by bodybuilders, although we have not found any histologic report on such a phenomenon. We report a case of a granulomatous reaction in a 30-year-old male bodybuilder having undergone anabolic steroid injections. Foreign bodies typical of steroids were found, together with areas of calcification and ossification with lamellar bone. Multivacuolated macrophages were found in the fibrous tissue close to these areas of calcification and ossification.

Populo H.,University of Porto | Caldas R.,University of Porto | Lopes J.M.,University of Porto | Pardal J.,Service of Anatomic Pathology | And 2 more authors.
Expert Opinion on Therapeutic Targets | Year: 2015

Objective: We aimed to verify if there is evidence to consider dichloroacetate (DCA), which inhibits the pyruvate dehydrogenase kinase (PDK) and reverts the metabolic shift of cancer cells from glycolysis to oxidative phosphorylation, as a promising drug for therapy of cutaneous melanoma (CM) patients. Research design and methods: We assessed the expression profile of PDK 1, 2 and 3 in a series of melanoma samples, to verify if melanoma tumors express the DCA targets, if this expression correlates with the activation of important signaling cascades for melanomagenesis and also with the prognosis of melanoma patients. We also established the sensitivity of melanoma cell lines to DCA treatment, by assessing their metabolic alterations, proliferation and survival. Results: We observed that both PDK 1 and 2 isoforms are overexpressed in CM compared to nevi, this expression being associated with the expression of the mTOR pathway effectors and independent of the BRAF mutational status. Melanoma cell lines treated with DCA showed a shift in metabolism, that is, a decrease in glucose consumption and lactate production, downregulation of proliferation, an increase of apoptosis and a decrease in mTOR pathway activation. Conclusion: Our results suggest that PDK expression may play a role in melanoma development and that DCA can be useful for CM therapy, alone or in combination with mTOR inhibitors. © 2015 Informa UK, Ltd.

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