Time filter

Source Type

Faure C.,Service dHistologie Embryologie Cytogenetique CECOS | Faure C.,University of Paris 13 | Dupont C.,Service dHistologie Embryologie Cytogenetique CECOS | Dupont C.,University of Paris 13 | And 7 more authors.
PLoS ONE | Year: 2014

Background: The impact of overweight among men of reproductive-age may affect fertility. Abdominal fat, more than body mass index, is an indicator of higher metabolic risk, which seems to be involved in decreasing sperm quality. This study aims to assess the relationship between abdominal fat and sperm DNA fragmentation and the effect of abdominal fat loss, among 6 men in subfertile couples. Methods: Sperm DNA fragmentation, abdominal fat and metabolic and hormonal profiles were measured in the 6 men before and after dietary advices. Seminal oxidative stress and antioxidant markers were determined. Results: After several months of a lifestyle program, all 6 men lost abdominal fat (patient 1: loss of 3 points of abdominal fat, patient 2: loss of 3 points, patient 3: loss of 2 points, patient 4: loss of 1 point, patient 5: loss of 4 points and patient 6: loss of 13 points). At the same time, their rate of sperm DNA fragmentation decreased: 9.5% vs 31%, 24% vs 43%, 18% vs 47%, 26.3% vs 66%, 25.4% vs 35% and 1.7% vs 25%. Also, an improvement in both metabolic (significant decrease in triglycerides and total cholesterol; p = 0.0139) and hormonal (significant increase in testosterone/oestradiol ratio; p = 0.0139) blood profiles was observed after following the lifestyle program. In seminal plasma, the amount of SOD2 has significantly increased (p = 0.0139) while in parallel carbonylated proteins have decreased. Furthermore, all spouses got pregnant. All pregnancies were brought to term. Conclusion: This study shows specifically that sperm DNA fragmentation among men in subfertile couples could be affected by abdominal fat, but improvement of lifestyle factor may correct this alteration. The effect of specific abdominal fat loss on sperm quality needs further investigation. The reduction of oxidative stress may be a contributing factor. © 2014 Faure et al.


Sermondade N.,Service dHistologie Embryologie Cytogenetique CECOS | Sermondade N.,French Institute of Health and Medical Research | Faure C.,Service dHistologie Embryologie Cytogenetique CECOS | Faure C.,French Institute of Health and Medical Research | And 28 more authors.
Human Reproduction Update | Year: 2013

Background: The global obesity epidemic has paralleled a decrease in semen quality. Yet, the association between obesity and sperm parameters remains controversial. The purpose of this report was to update the evidence on the association between BMI and sperm count through a systematic review with meta-analysis. Methods: A systematic review of available literature (with no language restriction) was performed to investigate the impact of BMI on sperm count. Relevant studies published until June 2012 were identified from a Pubmed and EMBASE search. We also included unpublished data (n =717 men) obtained from the Infertility Center of Bondy, France. Abstracts of relevant articles were examined and studies that could be included in this review were retrieved. Authors of relevant studies for the meta-analysis were contacted by email and asked to provide standardized data. Results: A total of 21 studies were included in the meta-analysis, resulting in a sample of 13 077 men from the general population and attending fertility clinics. Data were stratified according to the total sperm count as normozoospermia, oligozoospermia and azoospermia. Standardized weighted mean differences in sperm concentration did not differ significantly across BMI categories. There was a J-shaped relationship between BMI categories and risk of oligozoospermia or azoospermia. Compared with men of normal weight, the odds ratio (95% confidence interval) for oligozoospermia or azoospermia was 1.15 (0.93-1.43) for underweight, 1.11 (1.01-1.21) for overweight, 1.28 (1.06-1.55) for obese and 2.04 (1.59-2.62) for morbidly obese men. Conclusions: Overweight and obesity were associated with an increased prevalence of azoospermia or oligozoospermia. The main limitation of this report is that studied populations varied, with men recruited from both the general population and infertile couples. Whether weight normalization could improve sperm parameters should be evaluated further. © The Author 2012. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.


Chavatte-Palmer P.,French National Institute for Agricultural Research | Chavatte-Palmer P.,Paris Observatory | Levy R.,Service dHistologie embryologie cytogenetique CECOS | Levy R.,French Institute of Health and Medical Research | And 2 more authors.
Gynecologie Obstetrique Fertilite | Year: 2012

The concept of reproduction without uterus, or ectogenesis, has long been considered a myth. Nowadays, however, the in vitro conception (IVF) and embryo culture before implantation are largely used in humans with more than 50,000 IVF and ICSI procedures yearly in France, but also in ruminants where about 400,000 bovine embryos are produced in vitro worldwide each year. In order to proceed with gestation, a 3D structure enabling implantation is needed. Ex-vivo implantation of human embryos was obtained both in a perfused ex-vivo uterus and in a 3 dimensional culture of endometrial cells, but these experiments were stopped because of ethical concerns. The implantation of a mouse embryo in a similar 3D structure has been reported but did not lead to the production of a live pup. Another interest for an artificial uterus or placenta would be to use it for ex-vivo maturation of very premature fetuses. Extra-corporal membrane oxygenation (ECMO) was developed for many years but its use remains disappointing in preterm infants when compared to the important progress made with more classical clinical care. In any case, goat fetuses have been maintained alive up to 9 days in an artificial amniotic pouch, being oxygenated via ECMO. © 2011 Elsevier Masson SAS. All rights reserved.


Kashir J.,John Radcliffe Hospital | Sermondade N.,Service dHistologie Embryologie Cytogenetique CECOS | Sermondade N.,French Institute of Health and Medical Research | Sifer C.,Service dHistologie Embryologie Cytogenetique CECOS | And 7 more authors.
Human Reproduction | Year: 2012

Study Question Does motile sperm organelle morphology examination (MSOME) affect levels and localization patterns of the oocyte activation factor phospholipase C zeta (PLCζ) in globozoospermic sperm with and without an acrosomal bud? Summary Answer MSOME identified round-headed globozoospermic sperm with increased levels of PLCζ relative to sperm from the same sample that did not undergo MSOME, and identified novel patterns of PLCζ localization in sperm exhibiting an acrosomal bud. What is Known Already Absence or reduction in the level of PLCζ in the sperm head, abnormal localization patterning, or defective functional ability as a result of PLCζ gene mutation, have been linked to certain types of human male factor infertility in which oocyte activation is deficient. It has been determined that a subpopulation of sperm (1) from a patient exhibiting 100 globozoospermia presented with an acrosome bud upon MSOME. A cycle of intracytoplasmic morphologically selected sperm injection, carried out with sperm exhibiting an acrosomal bud led to pregnancy and birth of a healthy baby boy, without the use of assisted oocyte activation (AOA). Study Design, Size, Duration Immunofluorescent analysis of PLCζ in globozoospermic sperm from three patients, before and after MSOME. Participants/Materials, Setting , Methods Quantitative immunofluorescence was used to investigate PLCζ levels and localization patterns in individual sperm (n 1 patient) identified by MSOME and isolated by micromanipulation, and presenting with and without the acrosomal bud. A secondary aim was to investigate levels and localization patterns of PLCζ in sperm before and after MSOME from two other globozoospermic men. Main Results and The Role of ChanceNon-globozoospermic control sperm exhibited characteristic localization patterns of PLCζ immunofluorescence. Completely round-headed globozoospermic sperm from patients 1-3 were either devoid of PLCζ immunofluorescence, or exhibited an abnormal, punctate, pattern of PLCζ localization. PLCζ immunofluorescence in sperm exhibiting an acrosomal bud was observed in the midpiece with varying fluorescent intensity and was detected in 28.5 of such sperm. The majority of sperm with an acrosomal bud (43.0) exhibited punctate patterns of PLCζ localization within the sperm head. A further 28.5 of sperm exhibited PLCζ in both the head and the midpiece. Total levels of PLCζ, and the proportions of sperm exhibiting PLCζ immunoreactivity, showed significant variance (P ≤ 0.05) amongst control [45.8 arbitrary units (a.u.) and 95.7, respectively], non-MSOME-selected (25.9 a.u. and 46.1, respectively) and MSOME-selected globozoospermic sperm (33.4 a.u. and 65.0, respectively). Total levels of PLCζ immunofluorescence, and proportions of sperm exhibiting PLCζ immunoreactivity, in control sperm was significantly higher (P≤ 0.05) compared with non-MSOME-selected sperm, but not significantly different from MSOME-selected sperm. Limitations, Reasons for Caution The low numbers of sperm analysed may not be ideal for conclusive statistical analysis. Evaluation of the effects of MSOME on morphologically normal sperm would confirm Conclusions . Wider Implications of The Findings The present findings provide hope for the future treatment of globozoospermia without the need for AOA, and provide further evidence for the clinical application of PLCζ as a therapeutic and prognostic tool. Study Funding/Competing Interest (S)The research described herein was funded by the Nuffield Department of Obstetrics and Gynaecology, University of Oxford. The authors report no conflict of interest. © 2012 The Author.


Sermondade N.,Service dHistologie Embryologie Cytogenetique CECOS | Sermondade N.,University of Paris 13 | Massin N.,University Paris Est Creteil | Boitrelle F.,Hospital of Poissy | And 6 more authors.
Reproductive BioMedicine Online | Year: 2012

Recent studies have underlined the impact of obesity on sperm parameters, but very few data are available on the effect of weight loss on male fertility. This article reports the case series of three male patients who underwent rapid and major weight loss following bariatric surgery and the consequences of this surgery on semen parameters and fertility. A severe worsening of semen parameters was observed during the months after bariatric surgery, including extreme oligoasthenoteratozoospermia, but azoospermia was not observed. This effect may hypothetically be the result of two opposite mechanisms: (i) the suppression of the deleterious effects of obesity; and (ii) the negative impact of both nutritional deficiencies and the release of toxic substances. Information about potential reproductive consequences of bariatric surgery should be given to patients and sperm cryopreservation before surgery proposed. However, for one case, the alterations of spermatogenesis were reversible 2 years after the surgical procedure. Finally, intracytoplasmic sperm injection with fresh spermatozoa after male bariatric surgery can be successful, as demonstrated here, where clinical pregnancies were obtained for two out of the three couples. Recent studies have underlined the impact of obesity on sperm parameters, but very few data are available on the effect of weight loss on male fertility. We here report the case series of three male patients who underwent rapid and major weight loss following obesity surgery and the consequences of this on semen parameters and fertility. A severe worsening of semen parameters was observed during the months after surgery, but an absence of spermatozoa was not observed. This effect may hypothetically be the result of two opposite mechanisms, firstly the suppression of the deleterious effects of obesity and secondly the negative impact of both nutritional deficiencies and the release of toxic substances. Information about the potential reproductive consequences of obesity surgery should be given to patients and sperm cryopreservation before surgery proposed. However, for one case, the alterations of spermatogenesis were reversible 2 years after the surgical procedure. Finally, IVF with fresh spermatozoa after male bariatric surgery can be successful, as demonstrated in our case series, where clinical pregnancies were obtained for two of the three couples. © 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.


Chavatte-Palmer P.,CNRS Developmental Biology Laboratory | Tarrade A.,CNRS Developmental Biology Laboratory | Tarrade A.,Paris Observatory | Levy R.,Service dHistologie embryologie cytogenetique CECOS | Levy R.,University of Paris 13
Gynecologie Obstetrique Fertilite | Year: 2012

Many epidemiological studies indicate that environmental conditions during embryonic and fetal development can have an impact on health at adulthood. Animal studies clearly demonstrate that maternal, and even paternal undernutrition or nutritional excess durably modify some epigenetic marks in their offspring, affecting gene expression and physiological adaptations to the environment. It is crucial to better define the effects of early environment on adult phenotype and epigenetic marks in humans and to develop, with the help of animal models, new preventive strategies and treatments. © 2012 Elsevier Masson SAS. All rights reserved.


Sermondade N.,Service dHistologie Embryologie Cytogenetique CECOS | Sermondade N.,University of Paris 13 | Hafhouf E.,Service dHistologie Embryologie Cytogenetique CECOS | Dupont C.,Service dHistologie Embryologie Cytogenetique CECOS | And 8 more authors.
Human Reproduction | Year: 2011

We here report a successful pregnancy and healthy childbirth obtained in a case of total globozoospermia after intracytoplasmic morphologically selected sperm injection (IMSI) without assisted oocyte activation (AOA). Two semen analyses showed 100 globozoospermia on classic spermocytogram. Motile sperm organelle morphology examination (MSOME) analysis at ×10 000 magnification confirmed the round-headed aspect for 100 of sperm cells, but 1 of the spermatozoa seemed to present a small bud of acrosome. This particular aspect was confirmed by transmission electron microscopy and anti-CD46 staining analysis. Results from sperm DNA fragmentation and fluorescence in situ hybridization analyses were normal. The karyotype was 46XY, and no mutations or deletions in SPATA16 and DPY19L2 genes were detected. Considering these results, a single IMSI cycle was performed, and spermatozoa were selected for the absence of vacuoles and the presence of a small bud of acrosome. A comparable fertilization rate with or without calcium-ionophore AOA was observed. Two fresh top-quality embryos obtained without AOA were transferred at Day 2 after IMSI, leading to pregnancy and birth of a healthy baby boy. This successful outcome suggests that MSOME may be useful in cases of globozoospermia in order to carefully evaluate sperm morphology and to maximize the benefit of ICSI/IMSI. © 2011 The Author.


Sermondade N.,Service dHistologie embryologie cytogenetique CECOS | Sermondade N.,University of Paris 13 | Sifer C.,Service dHistologie embryologie cytogenetique CECOS
Gynecologie Obstetrique Fertilite | Year: 2011

Because it could constitute an additional tool for Assisted Reproductive Technologies decisions, Motile Sperm Organelle Morphology Examination (MSOME) has received an increased attention, especially for its applications for diagnosis and prognosis. On the methodological side, most of teams use a similar definition of a normal spermatozoon observed with MSOME. On the contrary, the classification of the abnormal sperms is less consensual because at least six classifications were published. No satisfying standard is published. Furthermore, the nature of vacuoles observed with MSOME is widely discussed. Initially considered as nuclear, some authors rather suggested an acrosomic origin, or a mixed origin, while the others give arguments for a nuclear origin. Moreover, very few data concerning thresholds are available and the MSOME indications are still controversial. So, the MSOME is a tool of which evaluation is in progress and its clinical interests are not demonstrated at the moment. © 2011 Elsevier Masson SAS. All rights reserved.


Whitfield M.,French Institute of Health and Medical Research | Pollet-Villard X.,Service dHistologie Embryologie Cytogenetique CECOS | Pollet-Villard X.,University of Paris 13 | Levy R.,Service dHistologie Embryologie Cytogenetique CECOS | And 3 more authors.
Asian Journal of Andrology | Year: 2015

Cholesterol is a key molecule in the mammalian physiology of especial particular importance for the reproductive system as it is the common precursor for steroid hormone synthesis. Cholesterol is also a recognized modulator of sperm functions, not only at the level of gametogenesis. Cholesterol homeostasis regulation is crucial for posttesticular sperm maturation, and imbalanced cholesterol levels may particularly affect these posttesticular events. Metabolic lipid disorders (dyslipidemia) affect male fertility but are most of the time studied from the angle of endocrine/testicular consequences. This review will focus on the deleterious effects of a particular dyslipidemia, i.e., hypercholesterolemia, on posttesticular maturation of mammalian spermatozoa.


Durand M.,Service dHistologie embryologie cytogenetique CECOS | Sifer C.,Service dHistologie embryologie cytogenetique CECOS
Gynecologie Obstetrique Fertilite | Year: 2013

The occurrence of complete fertilization failure following conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) is a rare event. Each of six physiological stages of the fertilisation can be failing. The cause of a fertilisation failure is either the spermatozoa, or the oocyte. The aim of this study was to determine if this event is predictable, and to define the later strategy. On all sperm tests available, it seems that only, tests of zona pellucida binding and acrosomique reaction are relatively predictive of a complete fertilization failure after IVF. No test allows at the moment to predict an oocyte contribution to unsuccessful fertilization. The strategy retained after a complete fertilization failure following the first attempt of IVF is to propose an ICSI; either to do an IVF if this event arises in the rank n+1, or when the number of inseminated oocytes was low. Concerning the ICSI, the low number of micro-injected oocyte is often in question; so the strategy retained following a first fertilization failure after ICSI is to perform a new ICSI attempt after optimization of the ovarian stimulation. When the cause is spermatic, it is indicated trying a modified ICSI with the use of pentoxifiline, or an intracytoplasmic morphologically selected sperm injection (IMSI), sometimes with assisted oocyte activation. In last solution, we can propose the ovum donation.

Loading Service dHistologie Embryologie Cytogenetique CECOS collaborators
Loading Service dHistologie Embryologie Cytogenetique CECOS collaborators