Entity

Time filter

Source Type


Dewalque L.,Service de Toxicologie Clinique | Charlier C.,Service de Toxicologie Clinique | Charlier C.,University of Liege
Revue Medicale de Liege | Year: 2012

Endocrine disruptors are chemicals substances interfering with the hormonal system. These pollutants, present in environment, can lead to diseases in human being. In this article, we take an interest to some endocrine disrupting substances linked to decrease in sperm quality and testicular dysgenesis syndrome, two pathologies involve in masculine fertility decline. The role of environment in complex diseases as male hypofertility is questioned.


Dubois N.,Service de Toxicologie Clinique | Paccou A.P.,Waters NV SA | De Backer B.G.,Service de Toxicologie Clinique | Charlier C.J.,Service de Toxicologie Clinique | Charlier C.J.,University of Liege
Journal of Analytical Toxicology | Year: 2012

In Belgium, driving under the influence (DUI) of cannabis is prohibited and has severe legal consequences for the driver if the blood plasma concentration of Δ9-tetrahydrocannabinol (THC) exceeds 1μmg/L. A method to quantify low concentrations of THC and its hydroxylated (THC-OH) and carboxylated (THC-COOH) metabolites in plasma was developed for DUI but also for other applications. Ultrahigh-performance liquid chromatography coupled to mass spectrometry seems to be a very convenient method to combine fast chromatographic separation and good sensitivity. The method was validated according to total error approach. Chromatographic separation was achieved in a 3-min total run time. The limits of quantitation were lower or equal to 1μmg/L for all compounds. The linearity of the method was acceptable in the validated range of concentrations (from 0.5 to 50μmg/L for THC, from 0.9 to 50 μg/L for THC-OH and from 1.1 to 100μmg/L for THC-COOH). The biases were lower than 13%, and the relative standard deviations for repeatability and intermediate precision did not exceed 15%. Lower and upper β-expectation tolerance limits did not exceed the acceptance limits of 20% for concentrations higher than 2μmg/L for THC and THC-OH and higher than 4μmg/L for THC-COOH. The acceptance limits were 30% for THC and THC-OH concentrations lower than 2μmg/L and for THC-COOH concentrations lower than 4μmg/L. © The Author [2012]. Published by Oxford University Press. All rights reserved.


Dubois N.,Service de Toxicologie Clinique | Hallet C.,Service dAnesthesie et de Reanimation | Luppens D.,Medecin Specialiste en Formation | Ansseau M.,Service de Psychiatrie et de Psychologie Medicale | Charlier C.,Service de Toxicologie Clinique
Revue Medicale de Liege | Year: 2013

Rapid Opiate Detoxification under Anesthesia (RODA) involves the use of opiate antagonists combined with anesthesia and pharmacotherapy to reduce withdrawal symptoms. The aim of our study was to measure the plasma concentrations of heroin metabolites and methadone during anesthesia and patient stay at the hospital in order to assess the amount of active substances at each protocol step. Plasma concentrations of antagonists were also quantified and compared to the recommended target values. Blood samples were drawn in 10 patients undergoing RODA at different times of the procedure (during anesthesia,in post-anesthesia care unit and in psychiatry unit). The plasma concentrations of heroin metabolites,methadone and antagonists were measured using a previously described method. Heroin active metabolites were no longer detected in the patient blood when he/she left the hospital; by contrast,methadone was still present at significant concentrations 3 days after the beginning of the detoxification procedure. Naltrexone analysis allowed us to adjust doses to insure opiate receptor blockade during acute withdrawal,which is a critical period.


Biological monitoring, also called biomonitoring, is a process to prevent and assess health risk for individuals exposed to chemical products present in environment or through workplace exposure. Biomonitoring is most often a monitoring of exposure or of effect. The exposure monitoring is currently the most widespread in toxicology. It involves the determination in biological fluids of different biomarkers, most of which are biomarkers of internal dose. These biological indicators are typically measured in blood and urine, but other biological samples can be analyzed. They are used to assess the penetration of environmental pollutants into the body. Assay results are interpreted in relation to reference values which are adapted either to occupationally exposed populations, or to general population. This interpretation and the choice of appropriate biomarker of exposure are not always obvious. Biomonitoring has some limitations despite its many advantages. It is complementary to another health prevention approach: the monitoring of ambient air. To illustrate in practice the biomonitoring of exposure, several examples of toxics and their associated biomarkers are reviewed: benzene, toluene, styrene, polycyclic aromatic hydrocarbons, chloroform, 2-hexanone and hydrogen cyanide.


Dubois N.,Service de Toxicologie Clinique | Debrus B.,University of Liege | Hubert Ph.,University of Liege | Charlier C.,Service de Toxicologie Clinique | Charlier C.,University of Liege
Acta Clinica Belgica | Year: 2010

Simultaneous determination of cocaine, opiates and amphetamines in serum by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) allowed to replace favourably gas chromatography coupled to mass spectrometry (GC-MS) used until now in our laboratory. It had to answer to accreditation demand according to Belgian Accreditation (Belac). Twenty-one deutereted internal standards were added to 500μL of serum. Sample pre-treatment consisted of solid-phase extraction using Oasis MCX cartridges 1mL, 30mg (Waters, Zellik, Belgium). Chromatographic separation was done on an Acquity HSS T3 column (2.1 x 100mm, 1.8μm, Waters). Mobile phase consisted of pH 3 ammonium formate buffer and of methanol adjusted to pH 3 with formic acid. Compounds were next analysed by tandem mass spectrometry operated in the multiple reaction monitoring (MRM) mode. The method was validated using total error approach. Twenty-seven drugs were separated in 19 minutes. The linearity of the method was acceptable in the validated range of concentrations. The bias and the relative standard deviations for repeatability and intermediate precision were acceptable. Lower and upper β-expectation tolerance limits did not exceed the acceptance limits of 20% for concentrations upper than 20μg/L and 50% for concentrations lower than 20μg/L. The limits of quantitation were lower than 7μg/L for all compounds.

Discover hidden collaborations